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Literature summary extracted from
- Diversity of DELTA12 fatty acid desaturases in santalaceae and their role in production of seed oil acetylenic fatty acids (2013), J. Biol. Chem., 288, 32405-32413.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.6 | expressed in Saccharomyces cerevisiae | Exocarpos cupressiformis |
| 1.14.19.6 | expressed in Saccharomyces cerevisiae | Santalum acuminatum |
| 1.14.19.B9 | gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid | Santalum acuminatum |
| 1.14.19.B9 | gene FADX-1A, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid | Exocarpos cupressiformis |
| 1.14.19.33 | gene FADX, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid | Santalum acuminatum |
| 1.14.19.33 | gene FADX-1A, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid | Exocarpos cupressiformis |
| 1.14.19.33 | gene FADX-1B, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid | Exocarpos cupressiformis |
| 1.14.19.33 | gene FADX-2, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, recombinant expression in Saccharomyces cerevisiae strains ole1 and INVSc1 in microsomes. Santalaceae FADX expressed in yeast has no detectable DELTA12 desaturase activity either acting on native fatty acids or when supplied with exogenous substrates but instead shows cis-DELTA13 desaturation activity on stearolic acid | Exocarpos cupressiformis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.B9 | microsome | - |
Exocarpos cupressiformis | - |
- |
| 1.14.19.B9 | microsome | - |
Santalum acuminatum | - |
- |
| 1.14.19.33 | microsome | - |
Exocarpos cupressiformis | - |
- |
| 1.14.19.33 | microsome | - |
Santalum acuminatum | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.6 | oleic acid + reduced acceptor + O2 | Exocarpos cupressiformis | best substrate with 23% conversion | linoleic acid + acceptor + H2O | - |
? |  |
| 1.14.19.6 | oleic acid + reduced acceptor + O2 | Santalum acuminatum | best substrate with 63.3% conversion | linoleic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | palmitoleic acid + reduced acceptor + O2 | Santalum acuminatum | 26.7% conversion | (9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | palmitoleic acid + reduced acceptor + O2 | Exocarpos cupressiformis | 4.1% conversion | (9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | stearolic acid + reduced acceptor + O2 | Exocarpos cupressiformis | 4.8% conversion | trans-12-octadecen-9-ynoic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | stearolic acid + reduced acceptor + O2 | Santalum acuminatum | 35% conversion | trans-12-octadecen-9-ynoic acid + acceptor + H2O | - |
? | |
| 1.14.19.B9 | additional information | Exocarpos cupressiformis | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | ? | - |
? | |
| 1.14.19.B9 | additional information | Santalum acuminatum | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | ? | - |
? | |
| 1.14.19.33 | additional information | Exocarpos cupressiformis | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | ? | - |
? | |
| 1.14.19.33 | additional information | Santalum acuminatum | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.6 | Exocarpos cupressiformis | - |
- |
- |
| 1.14.19.6 | Santalum acuminatum | - |
sweet quandong | - |
| 1.14.19.B9 | Exocarpos cupressiformis | U5LP87 | FADX-1A | - |
| 1.14.19.B9 | Santalum acuminatum | U5LRN8 | - |
- |
| 1.14.19.33 | Exocarpos cupressiformis | U5LN76 | FADX-1B | - |
| 1.14.19.33 | Exocarpos cupressiformis | U5LP51 | FADX-2 | - |
| 1.14.19.33 | Exocarpos cupressiformis | U5LP87 | FADX-1A | - |
| 1.14.19.33 | Santalum acuminatum | U5LRN8 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.B9 | seed | developing seeds | Exocarpos cupressiformis | - |
| 1.14.19.B9 | seed | developing seeds | Santalum acuminatum | - |
| 1.14.19.33 | seed | developing seeds | Exocarpos cupressiformis | - |
| 1.14.19.33 | seed | developing seeds | Santalum acuminatum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.6 | oleic acid + reduced acceptor + O2 | best substrate with 23% conversion | Exocarpos cupressiformis | linoleic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | oleic acid + reduced acceptor + O2 | best substrate with 63.3% conversion | Santalum acuminatum | linoleic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | palmitoleic acid + reduced acceptor + O2 | 26.7% conversion | Santalum acuminatum | (9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | palmitoleic acid + reduced acceptor + O2 | 4.1% conversion | Exocarpos cupressiformis | (9Z,12Z)-hexadeca-9,12-dienoic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | stearolic acid + reduced acceptor + O2 | 4.8% conversion | Exocarpos cupressiformis | trans-12-octadecen-9-ynoic acid + acceptor + H2O | - |
? | |
| 1.14.19.6 | stearolic acid + reduced acceptor + O2 | 35% conversion | Santalum acuminatum | trans-12-octadecen-9-ynoic acid + acceptor + H2O | - |
? | |
| 1.14.19.B9 | additional information | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | Exocarpos cupressiformis | ? | - |
? | |
| 1.14.19.B9 | additional information | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | Santalum acuminatum | ? | - |
? | |
| 1.14.19.B9 | octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Santalum acuminatum | (13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.B9 | octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | 18.7% conversion | Exocarpos cupressiformis | (13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.33 | additional information | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | Exocarpos cupressiformis | ? | - |
? | |
| 1.14.19.33 | additional information | the FADX group enzymes have no detectable DELTA12 FAD activity but instead catalyze cis-DELTA13 desaturation of stearolic acid | Santalum acuminatum | ? | - |
? | |
| 1.14.19.33 | octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Santalum acuminatum | (13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.33 | octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | 0.7% conversion | Exocarpos cupressiformis | (13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.33 | octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | 0.8% conversion | Exocarpos cupressiformis | (13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.33 | octadec-9-ynoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ | 18.7% conversion | Exocarpos cupressiformis | (13Z)-octadecen-13-en-9-ynoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.6 | DELTA12 FAD | - |
Exocarpos cupressiformis |
| 1.14.19.6 | DELTA12 FAD | - |
Santalum acuminatum |
| 1.14.19.6 | DELTA12 fatty acid desaturase | - |
Exocarpos cupressiformis |
| 1.14.19.6 | DELTA12 fatty acid desaturase | - |
Santalum acuminatum |
| 1.14.19.6 | FAD2 | - |
Exocarpos cupressiformis |
| 1.14.19.6 | FAD2 | - |
Santalum acuminatum |
| 1.14.19.B9 | DELTA13 desaturase | - |
Exocarpos cupressiformis |
| 1.14.19.B9 | DELTA13 desaturase | - |
Santalum acuminatum |
| 1.14.19.B9 | DELTA13 FAD | - |
Exocarpos cupressiformis |
| 1.14.19.B9 | DELTA13 FAD | - |
Santalum acuminatum |
| 1.14.19.B9 | FADX | - |
Santalum acuminatum |
| 1.14.19.B9 | FADX-1A | - |
Exocarpos cupressiformis |
| 1.14.19.33 | FADX | - |
Santalum acuminatum |
| 1.14.19.33 | FADX-1A | - |
Exocarpos cupressiformis |
| 1.14.19.33 | FADX-1B | - |
Exocarpos cupressiformis |
| 1.14.19.33 | FADX-2 | - |
Exocarpos cupressiformis |
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