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Literature summary extracted from

  • Young, H.E.; Donohue, M.P.; Smirnova, T.I.; Smirnov, A.I.; Zhou, P.
    The UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis utilizes Mn2+ cluster for catalysis (2013), J. Biol. Chem., 288, 26987-27001.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.54 additional information enzyme is active without addition of detergent Haemophilus influenzae
3.6.1.54 Triton X-100 stimulation in hydrolysis as the detergent concentration approaches the critical micelle concentration of 0.19 mM Haemophilus influenzae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.54
-
 Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.54 D117A 2fold decrease in activity compared to wild-type Haemophilus influenzae
3.6.1.54 D42A 80000fold decrease in activity compared to wild-type Haemophilus influenzae
3.6.1.54 D9A 200000fold decrease in activity compared to wild-type Haemophilus influenzae
3.6.1.54 H115A 12000fold decrease in activity compared to wild-type Haemophilus influenzae
3.6.1.54 H11A 20000fold decrease in activity compared to wild-type Haemophilus influenzae
3.6.1.54 H196A 5000fold decrease in activity compared to wild-type Haemophilus influenzae
3.6.1.54 R81A 7000fold decrease in activity compared to wild-type Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.54 0.0079
-
 UDP-2,3-diacylglucosamine pH 8.0, 30°C Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.54 Co2+ 10-50fold stimulation compared to conditions without metal additive Haemophilus influenzae
3.6.1.54 Fe3+ 10-50fold stimulation compared to conditions without metal additive Haemophilus influenzae
3.6.1.54 Mn2+ 600fold stimulation in presence of Mn2+, optimum concentraion 2.5 mM. Presence of a di-Mn2+ cluster Haemophilus influenzae
3.6.1.54 Ni2+ 10-50fold stimulation compared to conditions without metal additive Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.54 Haemophilus influenzae P44046
-
-
3.6.1.54 Haemophilus influenzae DSM 11121 P44046
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.54 7.8
-
 pH 8.0, 30°C Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.54 UDP-2,3-diacylglucosamine + H2O
-
 Haemophilus influenzae 2,3-diacylglucosamine 1-phosphate + UMP
-
 ?
3.6.1.54 UDP-2,3-diacylglucosamine + H2O
-
 Haemophilus influenzae DSM 11121 2,3-diacylglucosamine 1-phosphate + UMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.54 LpxH
-
 Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.54 8
-
 assay at. Enzyme is most active at slightly alkaline pH values and exhibits a sharp decrease in its catalytic activity at low pH Haemophilus influenzae