BRENDA - Enzyme Database

Listeria monocytogenes aguA1, but not aguA2, encodes a functional agmatine deiminase: biochemical characterization of its catalytic properties and roles in acid tolerance

Cheng, C.; Chen, J.; Fang, C.; Xia, Y.; Shan, Y.; Liu, Y.; Wen, G.; Song, H.; Fang, W.; J. Biol. Chem. 288, 26606-26615 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.3.12
expressed in Escherichia coli Rosetta (DE3) cells
Listeria monocytogenes
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.12
C157G
the mutant of inactive isoform AguA2 shows wild type enzyme activity
Listeria monocytogenes
3.5.3.12
C356A
inactive
Listeria monocytogenes
3.5.3.12
D218A
inactive
Listeria monocytogenes
3.5.3.12
D218E
inactive
Listeria monocytogenes
3.5.3.12
D94A
inactive
Listeria monocytogenes
3.5.3.12
D94E
inactive
Listeria monocytogenes
3.5.3.12
E155A
inactive
Listeria monocytogenes
3.5.3.12
E155D
inactive
Listeria monocytogenes
3.5.3.12
G157C
inactive
Listeria monocytogenes
3.5.3.12
H216A
inactive
Listeria monocytogenes
3.5.3.12
H216R
inactive
Listeria monocytogenes
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
Co2+
-
Listeria monocytogenes
3.5.3.12
Cu2+
-
Listeria monocytogenes
3.5.3.12
additional information
no inhibitory effect on isoform AguA1 activity with Fe3+, Mg2+, and Ni2+
Listeria monocytogenes
3.5.3.12
Zn2+
-
Listeria monocytogenes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.65
-
agmatine
at 25°C and pH 7.5
Listeria monocytogenes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
46800
-
x * 46800, calculated from amino acid sequence
Listeria monocytogenes
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Listeria monocytogenes
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Listeria monocytogenes 10403S
-
N-carbamoylputrescine + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.3.12
Listeria monocytogenes
-
-
-
3.5.3.12
Listeria monocytogenes 10403S
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.3.12
nickel-chelated agarose gel column chromatography
Listeria monocytogenes
Storage Stability
EC Number
Storage Stability
Organism
3.5.3.12
25°C, purified enzyme, 6 h, 80% loss of activity
Listeria monocytogenes
3.5.3.12
4°C, purified enzyme, at least 1 month, 15% loss of activity
Listeria monocytogenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
734228
Listeria monocytogenes
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
734228
Listeria monocytogenes 10403S
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
additional information
L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity
734228
Listeria monocytogenes
?
-
-
-
-
3.5.3.12
additional information
L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity
734228
Listeria monocytogenes 10403S
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.5.3.12
?
x * 46800, calculated from amino acid sequence
Listeria monocytogenes
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.12
25
-
-
Listeria monocytogenes
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.3.12
5
35
about 65% activity at 5°C, about 90% activity at 15°C, 100% activity at 25°C, less than 40% activity at 35°C
Listeria monocytogenes
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.12
34.28
-
agmatine
at 25°C and pH 7.5
Listeria monocytogenes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
8.5
-
-
Listeria monocytogenes
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.12
3.5
10.5
more than 85% activity between pH 3.5 and 10.5
Listeria monocytogenes
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.5.3.12
0.034
-
at 25°C and pH 7.5
Listeria monocytogenes
Cu2+
3.5.3.12
0.25
-
at 25°C and pH 7.5
Listeria monocytogenes
Zn2+
3.5.3.12
2.89
-
at 25°C and pH 7.5
Listeria monocytogenes
Co2+
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
expressed in Escherichia coli Rosetta (DE3) cells
Listeria monocytogenes
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.3.12
C157G
the mutant of inactive isoform AguA2 shows wild type enzyme activity
Listeria monocytogenes
3.5.3.12
C356A
inactive
Listeria monocytogenes
3.5.3.12
D218A
inactive
Listeria monocytogenes
3.5.3.12
D218E
inactive
Listeria monocytogenes
3.5.3.12
D94A
inactive
Listeria monocytogenes
3.5.3.12
D94E
inactive
Listeria monocytogenes
3.5.3.12
E155A
inactive
Listeria monocytogenes
3.5.3.12
E155D
inactive
Listeria monocytogenes
3.5.3.12
G157C
inactive
Listeria monocytogenes
3.5.3.12
H216A
inactive
Listeria monocytogenes
3.5.3.12
H216R
inactive
Listeria monocytogenes
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.5.3.12
0.034
-
at 25°C and pH 7.5
Listeria monocytogenes
Cu2+
3.5.3.12
0.25
-
at 25°C and pH 7.5
Listeria monocytogenes
Zn2+
3.5.3.12
2.89
-
at 25°C and pH 7.5
Listeria monocytogenes
Co2+
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.3.12
Co2+
-
Listeria monocytogenes
3.5.3.12
Cu2+
-
Listeria monocytogenes
3.5.3.12
additional information
no inhibitory effect on isoform AguA1 activity with Fe3+, Mg2+, and Ni2+
Listeria monocytogenes
3.5.3.12
Zn2+
-
Listeria monocytogenes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.12
0.65
-
agmatine
at 25°C and pH 7.5
Listeria monocytogenes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.3.12
46800
-
x * 46800, calculated from amino acid sequence
Listeria monocytogenes
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.3.12
agmatine + H2O
Listeria monocytogenes
-
N-carbamoylputrescine + NH3
-
-
?
3.5.3.12
agmatine + H2O
Listeria monocytogenes 10403S
-
N-carbamoylputrescine + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.12
nickel-chelated agarose gel column chromatography
Listeria monocytogenes
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.5.3.12
25°C, purified enzyme, 6 h, 80% loss of activity
Listeria monocytogenes
3.5.3.12
4°C, purified enzyme, at least 1 month, 15% loss of activity
Listeria monocytogenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.3.12
agmatine + H2O
-
734228
Listeria monocytogenes
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
agmatine + H2O
-
734228
Listeria monocytogenes 10403S
N-carbamoylputrescine + NH3
-
-
-
?
3.5.3.12
additional information
L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity
734228
Listeria monocytogenes
?
-
-
-
-
3.5.3.12
additional information
L-arginine does not act as the substrate for isoform AguA1. The recombinant isoform AguA2 shows no deiminase activity
734228
Listeria monocytogenes 10403S
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.3.12
?
x * 46800, calculated from amino acid sequence
Listeria monocytogenes
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.3.12
25
-
-
Listeria monocytogenes
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.5.3.12
5
35
about 65% activity at 5°C, about 90% activity at 15°C, 100% activity at 25°C, less than 40% activity at 35°C
Listeria monocytogenes
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.12
34.28
-
agmatine
at 25°C and pH 7.5
Listeria monocytogenes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.3.12
8.5
-
-
Listeria monocytogenes
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.3.12
3.5
10.5
more than 85% activity between pH 3.5 and 10.5
Listeria monocytogenes
Expression
EC Number
Organism
Commentary
Expression
3.5.3.12
Listeria monocytogenes
transcription is significantly induced at pH 5.0
up
General Information
EC Number
General Information
Commentary
Organism
3.5.3.12
malfunction
deletion of aguA1 significantly impairs Listeria monocytogenes survival both in gastric fluid at pH 2.5 and in mouse stomach
Listeria monocytogenes
3.5.3.12
physiological function
the enzyme mediates acid tolerance in Listeria monocytogenes
Listeria monocytogenes
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.3.12
malfunction
deletion of aguA1 significantly impairs Listeria monocytogenes survival both in gastric fluid at pH 2.5 and in mouse stomach
Listeria monocytogenes
3.5.3.12
physiological function
the enzyme mediates acid tolerance in Listeria monocytogenes
Listeria monocytogenes
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.5.3.12
Listeria monocytogenes
transcription is significantly induced at pH 5.0
up
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.3.12
53
-
agmatine
at 25°C and pH 7.5
Listeria monocytogenes
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.5.3.12
53
-
agmatine
at 25°C and pH 7.5
Listeria monocytogenes