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Literature summary extracted from

  • Vivona, S.; Cipriano, D.J.; OLeary, S.; Li, Y.H.; Fenn, T.D.; Brunger, A.T.
    Disassembly of all SNARE complexes by N-ethylmaleimide-sensitive factor (NSF) is initiated by a conserved 1:1 interaction between alpha-soluble NSF attachment protein (SNAP) and SNARE complex (2013), J. Biol. Chem., 288, 24984-24991.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.4.6 SNARE complex SNARE-stimulated ATP hydrolysis, all four SNARE complexes similarly stimulate the steady-state rate of NSF-catalyzed ATP hydrolysis activity, suggesting that the stimulation of ATPase activity is independent of SNARE primary sequence variation and N-terminal domain architecture Cricetulus griseus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.4.6 recombinant expression of His6-tagged enzyme NSF in Escherichia coli strain BL21(DE3), coexpression of alpha-SNAP, VAMP2(1-96), syntaxin1A(1-265)-C145S/S249C/K253C, syntaxin1A(191-265)-C145S/S249C/K253C, and SNAP25A Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.4.6 additional information
-
additional information Michaelis-Menten kinetics Cricetulus griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.4.6 Mg2+ required Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.4.6 ATP + H2O Cricetulus griseus
-
ADP + phosphate
-
?
3.6.4.6 additional information Cricetulus griseus modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.4.6 Cricetulus griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.4.6 recombinant His6-tagged enzyme NSF from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.4.6 ATP + H2O
-
Cricetulus griseus ADP + phosphate
-
?
3.6.4.6 additional information modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism Cricetulus griseus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.6.4.6 N-ethylmaleimide-sensitive factor
-
Cricetulus griseus
3.6.4.6 NSF
-
Cricetulus griseus
3.6.4.6 NSF ATPase
-
Cricetulus griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.4.6 37
-
assay at Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.4.6 8
-
assay at Cricetulus griseus

General Information

EC Number General Information Comment Organism
3.6.4.6 metabolism vesicle trafficking in eukaryotic cells is facilitated by SNARE-mediated membrane fusion. The ATPase N-ethylmaleimide-sensitive factor, NSF, and the adaptor protein soluble NSF attachment protein, alpha-SNAP, disassemble all SNARE complexes formed throughout different pathways Cricetulus griseus
3.6.4.6 physiological function vesicle trafficking in eukaryotic cells is facilitated by SNARE-mediated membrane fusion. The ATPase N-ethylmaleimide-sensitive factor, NSF, and the adaptor protein soluble NSF attachment protein, alpha-SNAP, disassemble all SNARE complexes formed throughout different pathways. SNARE-stimulated ATP hydrolysis. Modeling of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding. Subsequent additional alpha-SNAP binding events may occur as part of a processive disassembly mechanism Cricetulus griseus