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Literature summary extracted from

  • Yamagami, R.; Yamashita, K.; Nishimasu, H.; Tomikawa, C.; Ochi, A.; Iwashita, C.; Hirata, A.; Ishitani, R.; Nureki, O.; Hori, H.
    The tRNA recognition mechanism of folate/FAD-dependent tRNA methyltransferase (TrmFO) (2012), J. Biol. Chem., 287, 42480-42494.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.74 0.0011
-
 tRNAPhe containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0012
-
 tRNAPhe with deletions of anticodon arm containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0013
-
 tRNAPhe with deletions of aminoacyl stem containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0013
-
 tRNAPhe mutant A58U containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0015
-
 tRNAPhe with deletions of D-arm containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0019
-
 tRNAPhe with deletions of aminoacyl stem and D-arm containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0021
-
 tRNAPhe mutant G57A containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0022
-
 tRNAPhe mutant A58G containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0026
-
 tRNAPhe nucleotides 44-76 containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus
2.1.1.74 0.0045
-
 tRNAPhe nucleotides 18-76 containing uridine at position 54 pH not specified in the publication, 60°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.74 Thermus thermophilus Q5SID2
-
-
2.1.1.74 Thermus thermophilus DSM 579 Q5SID2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.74 additional information the minimum substrate requirement exists in the T-arm structure. The positive determinants for TrmFO are the U54U55C56 sequence and G53-C61 base pair. The affinity of TrmFO for tRNA in the initial binding process is weak. The methylated tRNA is released before the structural change process. Residue A38 prevents incorrect methylation of U32 in the anticodon loop. The m1A58 modification clearly accelerates the TrmFO reaction, suggesting a synergistic effect of the 5U54,m1A58, and s2U54 modifications on 5s2U54 formation. No substrates: tRNAPhe mutants U55C, C56U, U59C, U60C, U60G, G53C/C61G, G53A/C61U, G53A/C61A, G53A, G53U, C61A, C61U Thermus thermophilus ?
-
 ?
2.1.1.74 additional information the minimum substrate requirement exists in the T-arm structure. The positive determinants for TrmFO are the U54U55C56 sequence and G53-C61 base pair. The affinity of TrmFO for tRNA in the initial binding process is weak. The methylated tRNA is released before the structural change process. Residue A38 prevents incorrect methylation of U32 in the anticodon loop. The m1A58 modification clearly accelerates the TrmFO reaction, suggesting a synergistic effect of the 5U54,m1A58, and s2U54 modifications on 5s2U54 formation. No substrates: tRNAPhe mutants U55C, C56U, U59C, U60C, U60G, G53C/C61G, G53A/C61U, G53A/C61A, G53A, G53U, C61A, C61U Thermus thermophilus DSM 579 ?
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe containing uridine at position 54
-
 Thermus thermophilus DSM 579 S-adenosyl-L-homocysteine + tRNAPhe containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe mutant A58G containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe mutant A58G containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe mutant A58G containing uridine at position 54
-
 Thermus thermophilus DSM 579 S-adenosyl-L-homocysteine + tRNAPhe mutant A58G containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe mutant A58U containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe mutant A58U containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe mutant A58U containing uridine at position 54
-
 Thermus thermophilus DSM 579 S-adenosyl-L-homocysteine + tRNAPhe mutant A58U containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe mutant G57A containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe mutant G57A containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe mutant G57A containing uridine at position 54
-
 Thermus thermophilus DSM 579 S-adenosyl-L-homocysteine + tRNAPhe mutant G57A containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe nucleotides 18-76 containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe nucleotides 18-76 containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe nucleotides 44-76 containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe nucleotides 44-76 containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe with deletions of aminoacyl stem and D-arm containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe with deletions of aminoacyl stem and D-arm containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe with deletions of aminoacyl stem containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe with deletions of aminoacyl stem containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe with deletions of anticodon arm containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe with deletions of anticodon armand D-arm containing ribothymidine at position 54
-
 ?
2.1.1.74 S-adenosyl-L-methionine + tRNAPhe with deletions of D-arm containing uridine at position 54
-
 Thermus thermophilus S-adenosyl-L-homocysteine + tRNAPhe with deletions of D-arm containing ribothymidine at position 54
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.74 TRMFO
-
 Thermus thermophilus