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Literature summary extracted from

  • Watanabe, S.; Morimoto, D.; Fukumori, F.; Shinomiya, H.; Nishiwaki, H.; Kawano-Kawada, M.; Sasai, Y.; Tozawa, Y.; Watanabe, Y.
    Identification and characterization of D-hydroxyproline dehydrogenase and DELTA1-pyrroline-4-hydroxy-2-carboxylate deaminase involved in novel L-hydroxyproline metabolism of bacteria: metabolic convergent evolution (2012), J. Biol. Chem., 287, 32674-32688.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.22 expressed in Escherichia coli DH5alpha cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.22 L-2-oxo-3-deoxyarabinonate competitive inhibition Pseudomonas putida
3.5.4.22 pyruvate competitive inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.22 0.693
-
 1-pyrroline-4-hydroxy-2-carboxylate at pH 8.0 and 30°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.22 35000
-
 8 * 35000, His6-tagged protein, SDS-PAGE Pseudomonas putida
3.5.4.22 35342
-
 8 * 35342, calculated from amino acid sequence Pseudomonas putida
3.5.4.22 275000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.22 1-pyrroline-4-hydroxy-2-carboxylate + H2O Pseudomonas putida
-
 2,5-dioxopentanoate + NH3
-
 r
3.5.4.22 1-pyrroline-4-hydroxy-2-carboxylate + H2O Pseudomonas putida KT 2442
-
 2,5-dioxopentanoate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.22 Pseudomonas putida
-
-
-
3.5.4.22 Pseudomonas putida KT 2442
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.22 Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.22 1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida 2,5-dioxopentanoate + NH3
-
 r
3.5.4.22 1-pyrroline-4-hydroxy-2-carboxylate + H2O
-
 Pseudomonas putida KT 2442 2,5-dioxopentanoate + NH3
-
 r

Subunits

EC Number Subunits Comment Organism
3.5.4.22 homooctamer 8 * 35000, His6-tagged protein, SDS-PAGE Pseudomonas putida
3.5.4.22 homooctamer 8 * 35342, calculated from amino acid sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.5.4.22 DELTA1-pyrroline-4-hydroxy-2-carboxylate deaminase
-
 Pseudomonas putida
3.5.4.22 LhpC
-
 Pseudomonas putida
3.5.4.22 Pyr4H2C deaminase
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.22 1.19
-
 1-pyrroline-4-hydroxy-2-carboxylate at pH 8.0 and 30°C Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.4.22 0.492
-
 pyruvate at pH 8.0 and 30°C Pseudomonas putida
3.5.4.22 0.592
-
 L-2-oxo-3-deoxyarabinonate at pH 8.0 and 30°C Pseudomonas putida

General Information

EC Number General Information Comment Organism
3.5.4.22 malfunction disruption of the enzyme genes leads to loss of growth on L-hydroxyproline (as well as D-hydroxyproline) but not L- and D-proline Pseudomonas putida