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Literature summary extracted from

  • Smith, C.A.; Toth, M.; Frase, H.; Byrnes, L.J.; Vakulenko, S.B.
    Aminoglycoside 2-phosphotransferase IIIa (APH(2)-IIIa) prefers GTP over ATP: structural templates for nucleotide recognition in the bacterial aminoglycoside-2 kinases (2012), J. Biol. Chem., 287, 12893-12903.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.190
-
 Enterococcus gallinarum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.190 to 2.2 A resolution. Access to the ATP-binding template is blocked by a bulky tyrosine residue. Substitution of this tyrosine by a smaller amino acid opens access to the ATP template Enterococcus gallinarum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.190 Y92A residue Y92 blocks access to the ATP-binding template. Mutant shows 8fold decrease in km value for ATP Enterococcus gallinarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.190 0.04
-
 GTP wild-type, pH not specified in the publication, temperature not specified in the publication Enterococcus gallinarum
2.7.1.190 0.094
-
 GTP mutant Y92A, pH not specified in the publication, temperature not specified in the publication Enterococcus gallinarum
2.7.1.190 0.22
-
 ATP mutant Y92A, pH not specified in the publication, temperature not specified in the publication Enterococcus gallinarum
2.7.1.190 1.6
-
 ATP wild-type, pH not specified in the publication, temperature not specified in the publication Enterococcus gallinarum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.190 Enterococcus gallinarum P96762
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.190 ATP + kanamycin
-
 Enterococcus gallinarum ADP + kanamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin
-
 Enterococcus gallinarum GDP + kanamycin 2''-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.190 gentamicin resistance protein
-
 Enterococcus gallinarum