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Literature summary extracted from

  • Michel, C.; Van Echten-Deckert, G.; Rother, J.; Sandhoff, K.; Wang, E.; Merrill Jr., A.
    Characterization of ceramide synthesis. A dihydroceramide desaturase introduces the 4,5-trans-double bond of sphingosine at the level of dihydroceramide (1997), J. Biol. Chem., 272, 22432-22437.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.19.17 ascorbic acid inhibitory at high concentrations Rattus norvegicus
1.14.19.17 Cu2+ 25% inhibition at 1 mM Rattus norvegicus
1.14.19.17 cyanide
-
 Rattus norvegicus
1.14.19.17 dithiothreitol 81% inhibition at 1 mM Rattus norvegicus
1.14.19.17 additional information not inhibited by EDTA and Na2SO4 Rattus norvegicus
1.14.19.17 N-acetylcysteine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.17 0.12
-
 NADH with N-octanoyl-D-erythro-C18-sphinganine as cosubstrate, at pH 7.4 and 37°C Rattus norvegicus
1.14.19.17 0.34
-
 N-octanoyl-D-erythro-C18-sphinganine at pH 7.4 and 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.17 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.17 additional information not affected by Ca2+, Mg2+, Mn2+, Fe2+, K+, and Na+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.17 Rattus norvegicus Q564G3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.17 hepatocyte
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
1.14.19.17 -4°C, phosphate buffer (100 mM NaH2PO4/Na2HPO4, pH 7.4), 7 days, approximately 50% of the activity is lost Rattus norvegicus
1.14.19.17 -80°C, phosphate buffer (100 mM NaH2PO4/Na2HPO4, pH 7.4), 1 year, approximately 50% of the activity is lost Rattus norvegicus
1.14.19.17 37°C, phosphate buffer (100 mM NaH2PO4/Na2HPO4, pH 7.4), 3 h, approximately 50% of the activity is lost Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.17 dihydrosphingomyelin + NADH + O2 + H+ 20% activity compared to N-octanoyl-D-erythro-C18-sphinganine Rattus norvegicus ?
-
 ?
1.14.19.17 additional information no activity detected with dihydroglucosylceramide Rattus norvegicus ?
-
 ?
1.14.19.17 N-octanoyl-D-erythro-C18-sphinganine + NADH + O2 + H+
-
 Rattus norvegicus N-octanoyl-D-erythro-C18-sphingosine + NAD+ + H2O
-
 ?
1.14.19.17 N-octanoyl-D-erythro-C18-sphinganine + NADPH + O2 + H+
-
 Rattus norvegicus N-octanoyl-D-erythro-C18-sphingosine + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.17 dihydroceramide desaturase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.17 43
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.19.17 27 47 the half-maximal enzymatic activities are at 27°C and 47°C. Above 60°C, no activity is detected Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.19.17 6.5 9
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.17 cytochrome b5
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.19.17 0.031
-
 at pH 7.4 and 37°C Rattus norvegicus Cu2+
1.14.19.17 0.1
-
 at pH 7.4 and 37°C Rattus norvegicus cyanide