BRENDA - Enzyme Database show

Neuroprotective effects of nicotinamide N-methyltransferase and its metabolite 1-methylnicotinamide

Milani, Z.H.; Ramsden, D.B.; Parsons, R.B.; J. Biochem. Mol. Toxicol. 27, 451-456 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.1
recombinant ectopic expression of NNMT in enzyme-deficient SH-SY5Y human neuroblastoma cells increasing adenosine triphosphate synthesis and complex I activity
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
brain
the enzyme is robustly expressed in neurones in all regions of the brain
Homo sapiens
-
2.1.1.1
additional information
no enzyme activity in SH-SY5Y human neuroblastoma cells
Homo sapiens
-
2.1.1.1
neuron
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734104
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
recombinant ectopic expression of NNMT in enzyme-deficient SH-SY5Y human neuroblastoma cells increasing adenosine triphosphate synthesis and complex I activity
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
brain
the enzyme is robustly expressed in neurones in all regions of the brain
Homo sapiens
-
2.1.1.1
additional information
no enzyme activity in SH-SY5Y human neuroblastoma cells
Homo sapiens
-
2.1.1.1
neuron
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
734104
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.1.1.1
additional information
recombinant ectopic expression of NNMT in enzyme-deficient SH-SY5Y human neuroblastoma cells increased adenosine triphosphate synthesis and complex I activity, effects of which are replicated by the addition of 1-methylnicotinamide. The enzyme expression protects against the toxicity of mitochondrial toxins and abolishes the toxic effects of KCN, 2,4-dinitrophenol, and 6-hydroxydopamine, and reduced that of rotenone, while 1-methylnicotinamide significantly reduces the toxicity of rotenone, but has no effect on the toxicity of KCN, 2,4-dinitrophenol, and 6-hydroxydopamine. The enzyme is cytoprotective against toxins that inhibit various aspects of mitochondrial function, which are not mediated solely via increased 1-methylnicotinamide production, but in combination with other unidentified mechanisms
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.1
additional information
recombinant ectopic expression of NNMT in enzyme-deficient SH-SY5Y human neuroblastoma cells increased adenosine triphosphate synthesis and complex I activity, effects of which are replicated by the addition of 1-methylnicotinamide. The enzyme expression protects against the toxicity of mitochondrial toxins and abolishes the toxic effects of KCN, 2,4-dinitrophenol, and 6-hydroxydopamine, and reduced that of rotenone, while 1-methylnicotinamide significantly reduces the toxicity of rotenone, but has no effect on the toxicity of KCN, 2,4-dinitrophenol, and 6-hydroxydopamine. The enzyme is cytoprotective against toxins that inhibit various aspects of mitochondrial function, which are not mediated solely via increased 1-methylnicotinamide production, but in combination with other unidentified mechanisms
Homo sapiens