EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.63 | gene ecm, quantitative enzyme expression analysis, transcriptional profile of EMC pathway genes during the transition from growth on succinate to growth on ethylamine, overview. Recombinant expression in Escherichia coli | Methylorubrum extorquens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.63 | up | growth on ethylamide induces the enzyme, the ethylmalonyl-CoA mutase activity increases to a level sufficient for the observed growth rate at 9 h, which correlates with an upregulation of RNA transcripts for ecm and a decrease in the levels of ethylmalonyl-CoA | Methylorubrum extorquens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.63 | (2R)-ethylmalonyl-CoA | Methylorubrum extorquens | - |
(2S)-methylsuccinyl-CoA | - |
r | |
5.4.99.63 | (2R)-ethylmalonyl-CoA | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | - |
(2S)-methylsuccinyl-CoA | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.63 | Methylorubrum extorquens | C7C6S9 | gene ecm | - |
5.4.99.63 | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | C7C6S9 | gene ecm | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.63 | recombinant enzyme from Escherichia coli | Methylorubrum extorquens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.4.99.63 | culture condition:ethylamine-grown cell | wild-type Methylobacterium extorquens cells grown at steady state on a limiting concentration of succinate, are then switched to ethylamine as growth substrate, a condition where the cell must make a sudden switch from utilizing the tricarboxylic acid cycle to using the ethylmalonyl-CoA pathway for assimilation | Methylorubrum extorquens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.63 | (2R)-ethylmalonyl-CoA | - |
Methylorubrum extorquens | (2S)-methylsuccinyl-CoA | - |
r | |
5.4.99.63 | (2R)-ethylmalonyl-CoA | a reversible enzyme | Methylorubrum extorquens | (2S)-methylsuccinyl-CoA | - |
r | |
5.4.99.63 | (2R)-ethylmalonyl-CoA | - |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | (2S)-methylsuccinyl-CoA | - |
r | |
5.4.99.63 | (2R)-ethylmalonyl-CoA | a reversible enzyme | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | (2S)-methylsuccinyl-CoA | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.63 | Ecm | - |
Methylorubrum extorquens |
5.4.99.63 | ethylmalonyl coenzyme A mutase | - |
Methylorubrum extorquens |
5.4.99.63 | ethylmalonyl-CoA mutase | - |
Methylorubrum extorquens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.63 | adenosylcobalamin | dependent on | Methylorubrum extorquens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.63 | malfunction | overexpression of gene ecm removes control in the ethylmalonyl-CoA pathway | Methylorubrum extorquens |
5.4.99.63 | metabolism | the metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl-CoA pathway (EMC pathway), metabolic pathway for two carbon metabolism, overview. During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilatory flux to produce glyoxylate, malate, and succinate, metabolite profiles. Assimilatory carbon enters the ethylmalonyl-CoA pathway directly as acetyl-CoA, bypassing pathways for formaldehyde oxidation/assimilation and the regulatory mechanisms controlling them | Methylorubrum extorquens |
5.4.99.63 | physiological function | the ethylmalonyl-CoA pathway, ethylmalonyl-CoA mutase is a metabolic control point to restrict the production of glyoxylate | Methylorubrum extorquens |