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Literature summary extracted from

  • Vepachedu, V.R.; Ferry, J.G.
    Role of the fused corrinoid/methyl transfer protein CmtA during CO-dependent growth of Methanosarcina acetivorans (2012), J. Bacteriol., 194, 4161-4168.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.86 expressed in Escherichia coli Rosetta (DE3) cells Methanosarcina acetivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.86 0.135
-
 5-Methyl-5,6,7,8-tetrahydromethanopterin at 37°C in 50 mM MOPS-KOH (pH 7.0) Methanosarcina acetivorans
2.1.1.86 0.277
-
 coenzyme M at 37°C in 50 mM MOPS-KOH (pH 7.0) Methanosarcina acetivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.86 cytoplasm CmtA Methanosarcina acetivorans 5737
-
2.1.1.86 membrane MtrA-H Methanosarcina acetivorans 16020
-
2.1.1.86 soluble CmtA Methanosarcina acetivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.86 69000
-
 x * 69000, SDS-PAGE Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M Methanosarcina acetivorans
-
 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M Methanosarcina acetivorans
-
 5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.86 Methanosarcina acetivorans Q8THX4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.86 5,6,7,8-tetrahydromethanopterin + methylcob(III)alamin
-
 Methanosarcina acetivorans 5-methyl-5,6,7,8-tetrahydromethanopterin + cob(III)alamin
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
-
 Methanosarcina acetivorans 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
-
 ?
2.1.1.86 5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
-
 Methanosarcina acetivorans 5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.86 ? x * 69000, SDS-PAGE Methanosarcina acetivorans

Synonyms

EC Number Synonyms Comment Organism
2.1.1.86 5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase
-
 Methanosarcina acetivorans
2.1.1.86 5-methyl-5,6,7,8-tetrahydrosarcinapterin:coenzyme M methyltransferase
-
 Methanosarcina acetivorans
2.1.1.86 CH3-THMPT:HS-CoM methyltransferase
-
 Methanosarcina acetivorans
2.1.1.86 CH3-THMPT:HSCoM methyltransferase
-
 Methanosarcina acetivorans
2.1.1.86 CmtA
-
 Methanosarcina acetivorans
2.1.1.86 cytoplasmic methyltransferase A
-
 Methanosarcina acetivorans
2.1.1.86 MA4384
-
 Methanosarcina acetivorans
2.1.1.86 methyl-tetrahydromethanopterin:CoM methyltransferase
-
 Methanosarcina acetivorans
2.1.1.86 MtrA-H
-
 Methanosarcina acetivorans

General Information

EC Number General Information Comment Organism
2.1.1.86 physiological function CmtA is a soluble 5-methyl-5,6,7,8-tetrahydrosarcinapterin:coenzyme M methyltransferase postulated to supplement the membrane-bound 5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase during CO-dependent growth of Methanosarcina acetivorans Methanosarcina acetivorans