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Literature summary extracted from

  • Xavier, K.B.; Bassler, B.L.
    Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli (2005), J. Bacteriol., 187, 238-248.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.189 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.189 ATP + autoinducer-2 Escherichia coli i.e. AI-2 ADP + phosphorylated autoinducer-2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.189 Escherichia coli
-
 MG1655, gene lsrK
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.189 ATP + autoinducer-2 i.e. AI-2 Escherichia coli ADP + phosphorylated autoinducer-2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.189 AI-2 kinase
-
 Escherichia coli
2.7.1.189 lsrK
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.189 ATP
-
 Escherichia coli

Expression

EC Number Organism Comment Expression
2.7.1.189 Escherichia coli glycerol-3-phosphate inhibits lsr operon transcription activation by cAMP-catabolite activator protein, CAP. Dihydroxyacetone phosphate represses lsr transcription by a cAMP-CAP-independent mechanism involving LsrR, the lsr operon repressor, dihydroxyacetone phosphate causes repression by an LsrR-dependent and catabolite repression-independent route down
2.7.1.189 Escherichia coli cAMP-catabolite activator protein (CAP)-dependent activation of the lsr transcription. A cAMP-CAP-independent mechanism of lsr activation involves LsrR, the lsr operon repressor up

General Information

EC Number General Information Comment Organism
2.7.1.189 malfunction unlike the wild-type, the lsrK mutant does not activate transcription of the lsr operon in response to AI-2. The lsrK mutant fails to activate lsr expression because it cannot produce phospho-AI-2, which is the lsr operon inducer. Specifically, in an lsrK mutant, any AI-2 internalized by a secondary transporter does not get phosphorylated, and cannot be sequestered. In an lsrR/lsrK double mutant extracellular AI-2 accumulates to wild-type levels Escherichia coli
2.7.1.189 physiological function the enzyme is repsonsible for the regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli, overview. Enzyme LsrK and the Lsr transporter are required for AI-2 internalization and processing in Escherichia coli Escherichia coli