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Literature summary extracted from

  • Parales, R.E.; Emig, M.D.; Lynch, N.A.; Gibson, D.T.
    Substrate specificities of hybrid naphthalene and 2,4-dinitrotoluene dioxygenase enzyme systems (1998), J. Bacteriol., 180, 2337-2344.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.24 expressed in Escherichia coli JM109(DE3) cells Burkholderia sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.24 additional information engineering of hybrid dioxygenase enzymes coexpressing genes from naphthalene and 2,4-dinitrotoluene dioxygenases in Escherichia coli. In the active hybrids, replacement of small subunits affects the rate of product formation but has no effect on the substrate range, regiospecificity, or enantiomeric purity of oxidation products with the substrates tested. The small subunit of the oxygenase is essential for activity but does not play a major role in determining the specificity of these enzymes. Introduction of the small subunit of 2-nitrotolene synthase from Pseudomonas sp. strain JS42 leads to 30% reduction in product formation from naphthalene and dinitrotoluene Burkholderia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.24 50000
-
 x * 50000, SDS-PAGE Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2 Burkholderia sp.
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2 Burkholderia sp. DNT
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.24 Burkholderia sp.
-
-
-
1.14.12.24 Burkholderia sp. Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-
1.14.12.24 Burkholderia sp. DNT
-
-
-
1.14.12.24 Burkholderia sp. DNT Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.12.24 0.38
-
 with naphthalene as substrate, pH and temperature not specified in the publication Burkholderia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2,4-dinitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-nitrobenzyl alcohol + NAD+ + ?
-
 ?
1.14.12.24 2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 2-nitrobenzyl alcohol + NAD+ + ?
-
 ?
1.14.12.24 naphthalene + NADH + H+ + O2
-
 Burkholderia sp. (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
 ?
1.14.12.24 naphthalene + NADH + H+ + O2
-
 Burkholderia sp. DNT (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
-
 ?
1.14.12.24 naphthalene + NADH + H+ + O2
-
 Burkholderia sp. (1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
-
 ?
1.14.12.24 naphthalene + NADH + H+ + O2
-
 Burkholderia sp. DNT (1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.24 ? x * 50000, SDS-PAGE Burkholderia sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.12.24 DNTDO
-
 Burkholderia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.24 NADH
-
 Burkholderia sp.