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Literature summary extracted from

  • Suen, W.C.; Haigler, B.E.; Spain, J.C.
    2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase (1996), J. Bacteriol., 178, 4926-4934.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.24 expressed in Escherichia coli JM109(DE3) cells Burkholderia sp.
1.14.12.24 expression in Escherichia coli Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + O2 Burkholderia sp.
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.24 Burkholderia sp.
-
-
-
1.14.12.24 Burkholderia sp. Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + O2
-
 Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-nitrobenzyl alcohol + NAD+ + ?
-
 ?
1.14.12.24 3-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 3-nitrobenzyl alcohol + NAD+ + ?
-
 ?
1.14.12.24 4-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 4-nitrobenzyl alcohol + NAD+ + ?
-
 ?
1.14.12.24 acenaphthene + NADH + H+ + O2
-
 Burkholderia sp. 1-acenaphthenol + NAD+ + ?
-
 ?
1.14.12.24 indan + NADH + H+ + O2
-
 Burkholderia sp. 1-indanol + 1-indanone + indene + NAD+
-
 ?
1.14.12.24 indan + NADH + H+ + O2
-
 Burkholderia sp. 1-indanol + ? + NAD+ no formation of 1,2-dihydroxyindan ?
1.14.12.24 indene + NADH + H+ + O2
-
 Burkholderia sp. 1-indenol + 1,2-dihydroxyindan + NAD+
-
 ?
1.14.12.24 indole + NADH + H+ + O2
-
 Burkholderia sp. indigo + NAD+ + ?
-
 ?
1.14.12.24 indoline + NADH + H+ + O2
-
 Burkholderia sp. indigo + ? + NAD+
-
 ?
1.14.12.24 indoline + NADH + H+ + O2
-
 Burkholderia sp. indole + NAD+ + ?
-
 ?
1.14.12.24 additional information no activity with anisole, carbazole, and nitrobenzene Burkholderia sp. ?
-
 ?
1.14.12.24 naphthalene + NADH + H+ + O2
-
 Burkholderia sp. (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
-
 ?
1.14.12.24 naphthalene + O2
-
 Burkholderia sp. cis-1,2-dihydroxy-1,2-dihydronaphthalene product is formed in 92% enantiomeric excess ?
1.14.12.24 phenetole + NADH + H+ + O2
-
 Burkholderia sp. phenol + NAD+ + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.24 DNT dioxygenase
-
 Burkholderia sp.
1.14.12.24 dntA
-
 Burkholderia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.24 NADH
-
 Burkholderia sp.

General Information

EC Number General Information Comment Organism
1.14.12.24 physiological function an Escherichia coli clone that expresses DNT dioxygenase converts dinitrotoluene to 4-methyl-5-nitrocatechol and naphthalene to (+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene. The enzyme system exhibits similar substrate specificities as naphthalene dioxygenase, both can oxidize such compounds as indole, indan, indene, phenetole, and acenaphthene Burkholderia sp.