EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.2 | expressed in Escherichia coli Top10 cells | Blastobotrys adeninivorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.2 | 1,10-phenanthroline | 66% residual activity at 1 mM | Blastobotrys adeninivorans | |
3.5.4.2 | Cd2+ | 28% residual activity at 1 mM | Blastobotrys adeninivorans | |
3.5.4.2 | Cu2+ | 64% residual activity at 1 mM | Blastobotrys adeninivorans | |
3.5.4.2 | Fe2+ | 7% residual activity at 1 mM | Blastobotrys adeninivorans | |
3.5.4.2 | Mn2+ | 32% residual activity at 1 mM | Blastobotrys adeninivorans | |
3.5.4.2 | additional information | not inhibited by EDTA | Blastobotrys adeninivorans | |
3.5.4.2 | Ni2+ | 48% residual activity at 1 mM | Blastobotrys adeninivorans | |
3.5.4.2 | Zn2+ | 56% residual activity at 1 mM | Blastobotrys adeninivorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.2 | 0.47 | - |
2,6-diaminopurine | at pH 7.4 and 40°C | Blastobotrys adeninivorans | |
3.5.4.2 | 0.66 | - |
adenine | at pH 7.4 and 40°C | Blastobotrys adeninivorans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.4.2 | soluble | - |
Blastobotrys adeninivorans | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.4.2 | additional information | Mg2+, Mo2+, and Ca2+ have either no effect or only a weak effect on enzyme activity | Blastobotrys adeninivorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.2 | 40000 | - |
gel filtration | Blastobotrys adeninivorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.2 | adenine + H2O | Blastobotrys adeninivorans | 100% activity | hypoxanthine + NH3 | - |
? | |
3.5.4.2 | adenine + H2O | Blastobotrys adeninivorans LS3 | 100% activity | hypoxanthine + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.2 | Blastobotrys adeninivorans | L8BT48 | - |
- |
3.5.4.2 | Blastobotrys adeninivorans LS3 | L8BT48 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.2 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration | Blastobotrys adeninivorans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.4.2 | 1.88 | - |
after 5.26fold purification, at pH 7.4 and 40°C | Blastobotrys adeninivorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.2 | 2,6-diaminopurine + H2O | 73% activity compared to adenine | Blastobotrys adeninivorans | ? | - |
? | |
3.5.4.2 | 2,6-diaminopurine + H2O | 73% activity compared to adenine | Blastobotrys adeninivorans LS3 | ? | - |
? | |
3.5.4.2 | 2-amino-6-bromopurine + H2O | 109% activity compared to adenine | Blastobotrys adeninivorans | ? | - |
? | |
3.5.4.2 | 2-amino-6-bromopurine + H2O | 109% activity compared to adenine | Blastobotrys adeninivorans LS3 | ? | - |
? | |
3.5.4.2 | 6-chloropurine + H2O | 119% activity compared to adenine | Blastobotrys adeninivorans | ? | - |
? | |
3.5.4.2 | 6-chloropurine + H2O | 119% activity compared to adenine | Blastobotrys adeninivorans LS3 | ? | - |
? | |
3.5.4.2 | adenine + H2O | 100% activity | Blastobotrys adeninivorans | hypoxanthine + NH3 | - |
? | |
3.5.4.2 | adenine + H2O | 100% activity | Blastobotrys adeninivorans LS3 | hypoxanthine + NH3 | - |
? | |
3.5.4.2 | additional information | no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP | Blastobotrys adeninivorans | ? | - |
? | |
3.5.4.2 | additional information | no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP | Blastobotrys adeninivorans LS3 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.2 | monomer | 1 * 40000, SDS-PAGE | Blastobotrys adeninivorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.2 | aadA | - |
Blastobotrys adeninivorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.2 | 40 | - |
- |
Blastobotrys adeninivorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.4.2 | 30 | 50 | the enzyme loses 35% of its catalytic function in 1 h at 40°C, whereas at 30°C, the activity of the enzyme remains stable. At 50°C, the activity drops within 10 min to zero | Blastobotrys adeninivorans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.2 | 12.3 | - |
2,6-diaminopurine | at pH 7.4 and 40°C | Blastobotrys adeninivorans | |
3.5.4.2 | 20.8 | - |
adenine | at pH 7.4 and 40°C | Blastobotrys adeninivorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.2 | 6.5 | 7 | - |
Blastobotrys adeninivorans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.4.2 | 5.5 | 7.5 | the enzyme has maximal stability at 30°C for 24 h in HEPES buffer at pHs between 5.5 and 7.5 | Blastobotrys adeninivorans |