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Literature summary extracted from

  • Jankowska, D.A.; Faulwasser, K.; Trautwein-Schult, A.; Cordes, A.; Hoferichter, P.; Klein, C.; Bode, R.; Baronian, K.; Kunze, G.
    Arxula adeninivorans recombinant adenine deaminase and its application in the production of food with low purine content (2013), J. Appl. Microbiol., 115, 1134-1146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.2 expressed in Escherichia coli Top10 cells Blastobotrys adeninivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.2 1,10-phenanthroline 66% residual activity at 1 mM Blastobotrys adeninivorans
3.5.4.2 Cd2+ 28% residual activity at 1 mM Blastobotrys adeninivorans
3.5.4.2 Cu2+ 64% residual activity at 1 mM Blastobotrys adeninivorans
3.5.4.2 Fe2+ 7% residual activity at 1 mM Blastobotrys adeninivorans
3.5.4.2 Mn2+ 32% residual activity at 1 mM Blastobotrys adeninivorans
3.5.4.2 additional information not inhibited by EDTA Blastobotrys adeninivorans
3.5.4.2 Ni2+ 48% residual activity at 1 mM Blastobotrys adeninivorans
3.5.4.2 Zn2+ 56% residual activity at 1 mM Blastobotrys adeninivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.2 0.47
-
 2,6-diaminopurine at pH 7.4 and 40°C Blastobotrys adeninivorans
3.5.4.2 0.66
-
 adenine at pH 7.4 and 40°C Blastobotrys adeninivorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.2 soluble
-
 Blastobotrys adeninivorans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.2 additional information Mg2+, Mo2+, and Ca2+ have either no effect or only a weak effect on enzyme activity Blastobotrys adeninivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.2 40000
-
 gel filtration Blastobotrys adeninivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.2 adenine + H2O Blastobotrys adeninivorans 100% activity hypoxanthine + NH3
-
 ?
3.5.4.2 adenine + H2O Blastobotrys adeninivorans LS3 100% activity hypoxanthine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.2 Blastobotrys adeninivorans L8BT48
-
-
3.5.4.2 Blastobotrys adeninivorans LS3 L8BT48
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.2 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Blastobotrys adeninivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.2 1.88
-
 after 5.26fold purification, at pH 7.4 and 40°C Blastobotrys adeninivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.2 2,6-diaminopurine + H2O 73% activity compared to adenine Blastobotrys adeninivorans ?
-
 ?
3.5.4.2 2,6-diaminopurine + H2O 73% activity compared to adenine Blastobotrys adeninivorans LS3 ?
-
 ?
3.5.4.2 2-amino-6-bromopurine + H2O 109% activity compared to adenine Blastobotrys adeninivorans ?
-
 ?
3.5.4.2 2-amino-6-bromopurine + H2O 109% activity compared to adenine Blastobotrys adeninivorans LS3 ?
-
 ?
3.5.4.2 6-chloropurine + H2O 119% activity compared to adenine Blastobotrys adeninivorans ?
-
 ?
3.5.4.2 6-chloropurine + H2O 119% activity compared to adenine Blastobotrys adeninivorans LS3 ?
-
 ?
3.5.4.2 adenine + H2O 100% activity Blastobotrys adeninivorans hypoxanthine + NH3
-
 ?
3.5.4.2 adenine + H2O 100% activity Blastobotrys adeninivorans LS3 hypoxanthine + NH3
-
 ?
3.5.4.2 additional information no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP Blastobotrys adeninivorans ?
-
 ?
3.5.4.2 additional information no activity with guanine, adenosine, inosine, guanosine, xanthosine, 2-chloroadenine, 2-amino-7-deazapurine, FAD, and AMP Blastobotrys adeninivorans LS3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.2 monomer 1 * 40000, SDS-PAGE Blastobotrys adeninivorans

Synonyms

EC Number Synonyms Comment Organism
3.5.4.2 aadA
-
 Blastobotrys adeninivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.2 40
-
-
 Blastobotrys adeninivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.4.2 30 50 the enzyme loses 35% of its catalytic function in 1 h at 40°C, whereas at 30°C, the activity of the enzyme remains stable. At 50°C, the activity drops within 10 min to zero Blastobotrys adeninivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.4.2 12.3
-
 2,6-diaminopurine at pH 7.4 and 40°C Blastobotrys adeninivorans
3.5.4.2 20.8
-
 adenine at pH 7.4 and 40°C Blastobotrys adeninivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.2 6.5 7
-
 Blastobotrys adeninivorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.4.2 5.5 7.5 the enzyme has maximal stability at 30°C for 24 h in HEPES buffer at pHs between 5.5 and 7.5 Blastobotrys adeninivorans