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Literature summary extracted from

  • Kristiansen, A.; Grgic, M.; Altermark, B.; Leiros, I.
    Properties and distribution of a metallo-beta-lactamase (ALI-1) from the fish pathogen Aliivibrio salmonicida LFI1238 (2015), J. Antimicrob. Chemother., 70, 766-772.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 chromosome-encoded metallo-beta-lactamase, genotyping in Aliivibrio isolates, recombinant enzyme overexpression in Escherichia coli Aliivibrio salmonicida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 2-mercaptoethanol
-
 Aliivibrio salmonicida
3.5.2.6 DTT
-
 Aliivibrio salmonicida
3.5.2.6 TCEP complete inhibition Aliivibrio salmonicida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.006
-
 nitrocefin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.017
-
 ertapenem pH 7.2, 37°C, recombinant enzyme, in presence of NaCl Aliivibrio salmonicida
3.5.2.6 0.037
-
 ceftazidime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.068
-
 cefuroxime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.073
-
 ertapenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.079
-
 cefoxitin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.142
-
 meropenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.177
-
 cefepime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.933
-
 Imipenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.6 periplasm
-
 Aliivibrio salmonicida
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 NaCl 40% activity increase at 0.5 M Aliivibrio salmonicida
3.5.2.6 Zn2+ a zinc enzyme Aliivibrio salmonicida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.6 additional information Aliivibrio salmonicida the enzyme hydrolyses all beta-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Aliivibrio salmonicida B6EPH0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 recombinant enzyme from Escherichia coli Aliivibrio salmonicida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 cefepime + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 ertapenem + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 imipenem + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 meropenem + H2O
-
 Aliivibrio salmonicida ?
-
 ?
3.5.2.6 additional information the enzyme hydrolyses all beta-lactam antibiotics tested, except for the monobactam aztreonam and the penicillin piperacillin Aliivibrio salmonicida ?
-
 ?
3.5.2.6 additional information no activity with piperacillin and aztreonam Aliivibrio salmonicida ?
-
 ?
3.5.2.6 nitrocefin + H2O nitrocefin shows autohydrolysis at pH 7.5-9.0 in Tris buffer, while not in HEPES buffer with the same pH Aliivibrio salmonicida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 ALI-1
-
 Aliivibrio salmonicida
3.5.2.6 MBL
-
 Aliivibrio salmonicida
3.5.2.6 metallo-beta-lactamase
-
 Aliivibrio salmonicida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 35
-
-
 Aliivibrio salmonicida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.6 additional information
-
 temperature dependency at different NaCl concentrations, overview Aliivibrio salmonicida
3.5.2.6
-
 50 activity range, profile, overview Aliivibrio salmonicida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.09
-
 ceftazidime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.27
-
 cefoxitin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 0.58
-
 cefepime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 4
-
 ertapenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 4.3
-
 ertapenem pH 7.2, 37°C, recombinant enzyme, in presence of NaCl Aliivibrio salmonicida
3.5.2.6 6.3
-
 nitrocefin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 8
-
 Imipenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 11
-
 cefuroxime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 13
-
 meropenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 8 8.3
-
 Aliivibrio salmonicida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.6 additional information
-
 broad pH range Aliivibrio salmonicida

Expression

EC Number Organism Comment Expression
3.5.2.6 Aliivibrio salmonicida the marine enzyme is highly salt tolerant and cold active additional information

General Information

EC Number General Information Comment Organism
3.5.2.6 evolution metallo-beta-lactamases (MBLs) belong to the protein family of beta-lactamases, a group of enzymes that deactivate beta-lactam antibiotics by cleaving the amide bond in the beta-lactam ring, the enzyme belongs to the molecular class B, subclass B1. The Ambler class B beta-lactamase shares 39% and 29% amino acid identity with IMP-1 and VIM-2, from a Pseudomonas aeruginosa hospital isolate, respectively. Active-site residues that are coordinating zinc ions are conserved among all three subclasses Aliivibrio salmonicida
3.5.2.6 additional information the marine enzyme is highly salt tolerant and cold active Aliivibrio salmonicida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 2.4
-
 ceftazidime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 3.3
-
 cefepime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 3.4
-
 cefoxitin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 8.5
-
 Imipenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 54
-
 ertapenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 91.6
-
 meropenem pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 160
-
 cefuroxime pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida
3.5.2.6 250
-
 ertapenem pH 7.2, 37°C, recombinant enzyme, in presence of NaCl Aliivibrio salmonicida
3.5.2.6 1000
-
 nitrocefin pH 7.2, 37°C, recombinant enzyme Aliivibrio salmonicida