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Literature summary extracted from

  • Makena, A.; Brem, J.; Pfeffer, I.; Geffen, R.E.; Wilkins, S.E.; Tarhonskaya, H.; Flashman, E.; Phee, L.M.; Wareham, D.W.; Schofield, C.J.
    Biochemical characterization of New Delhi metallo-beta-lactamase variants reveals differences in protein stability (2015), J. Antimicrob. Chemother., 70, 463-469.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 gene blaNDM-1, genotyping, recombinant N-terminally His6-tagged enzyme expression in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae
3.5.2.6 gene blaNDM-10, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae
3.5.2.6 gene blaNDM-12, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-2, genotyping, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Acinetobacter baumannii
3.5.2.6 gene blaNDM-3, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-4, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-5, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-6, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-7, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-8, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli
3.5.2.6 gene blaNDM-9, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 A233V naturally occuring genetic variant Escherichia coli
3.5.2.6 D130G/M154L naturally occuring genetic variation Escherichia coli
3.5.2.6 D130N/M154L naturally occuring genetic variation Escherichia coli
3.5.2.6 D95N naturally occuring genetic variation Escherichia coli
3.5.2.6 E152K naturally occuring genetic variation Klebsiella pneumoniae
3.5.2.6 G69S/A74T/G200R naturally occuring genetic variation Klebsiella pneumoniae
3.5.2.6 M154L naturally occuring genetic variation Escherichia coli
3.5.2.6 M154L/G222D naturally occuring genetic variation Escherichia coli
3.5.2.6 P28A naturally occuring genetic variation, the P28A substitution occurs in the predicted N-terminal periplasmic signal peptide Acinetobacter baumannii
3.5.2.6 V88L/M154L naturally occuring genetic variation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 nitrocefin substrate inhibition; substrate inhibition; substrate inhibition; substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.004
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.005
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.005
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.006
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.008
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.01
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.011
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.011
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.013
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.015
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.016
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.019
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.02
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.023
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.027
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.029
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.043
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.045
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.049
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.05
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.054
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.056
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.057
-
 meropenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.058
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.062
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.063
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.064
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.078
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.079
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.082
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.083
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.086
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.099
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.1
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.107
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.11
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.115
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.119
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.119
-
 doripenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 0.119
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.125
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.126
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.133
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.144
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.148
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.151
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.156
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.207
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.229
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.258
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.305
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.455
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.6 periplasm
-
 Klebsiella pneumoniae
-
-
3.5.2.6 periplasm
-
 Acinetobacter baumannii
-
-
3.5.2.6 periplasm
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+ a zinc metalloenzyme Klebsiella pneumoniae
3.5.2.6 Zn2+ a zinc metalloenzyme Acinetobacter baumannii
3.5.2.6 Zn2+ a zinc metalloenzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Acinetobacter baumannii F2YZ26 gene blaNDM-2
-
3.5.2.6 Escherichia coli A0A024FRL9 gene blaNDM-12
-
3.5.2.6 Escherichia coli A0A0F6N6D4 gene blaNDM-5
-
3.5.2.6 Escherichia coli H6WET3 gene blaNDM-6
-
3.5.2.6 Escherichia coli H6WZS9 gene blaNDM-4
-
3.5.2.6 Escherichia coli I3VKD5 gene blaNDM-3
-
3.5.2.6 Escherichia coli J7I0S9 gene blaNDM-7
-
3.5.2.6 Escherichia coli M1VE66 gene blaNDM-8
-
3.5.2.6 Klebsiella pneumoniae C7C422 gene blaNDM-1
-
3.5.2.6 Klebsiella pneumoniae S5ZIP8 gene blaNDM-10
-
3.5.2.6 Klebsiella pneumoniae T2A6Y2 gene blaNDM-9
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the N-terminal His6-tag using recombinant human Rhinovirus 3C protease, and further purification of the untagged protein by affinity chromatography, followed by ultrafiltration Klebsiella pneumoniae
3.5.2.6 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the N-terminal His6-tag using recombinant human Rhinovirus 3C protease, and further purification of the untagged protein by affinity chromatography, followed by ultrafiltration Acinetobacter baumannii
3.5.2.6 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the N-terminal His6-tag using recombinant human Rhinovirus 3C protease, and further purification of the untagged protein by affinity chromatography, followed by ultrafiltration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Klebsiella pneumoniae (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Acinetobacter baumannii (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ampicillin + H2O
-
 Escherichia coli (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cefalotin + H2O
-
 Klebsiella pneumoniae (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefalotin + H2O
-
 Escherichia coli (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O low activity Klebsiella pneumoniae (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O low activity Escherichia coli (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O low activity Klebsiella pneumoniae (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 ceftazidime + H2O low activity Escherichia coli (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 doripenem + H2O
-
 Klebsiella pneumoniae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-([(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 doripenem + H2O
-
 Escherichia coli (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-([(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Klebsiella pneumoniae (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([2-[(iminomethyl)amino]ethyl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Escherichia coli (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([2-[(iminomethyl)amino]ethyl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Klebsiella pneumoniae (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Escherichia coli (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Klebsiella pneumoniae (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Escherichia coli (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 MBL
-
 Klebsiella pneumoniae
3.5.2.6 MBL
-
 Acinetobacter baumannii
3.5.2.6 MBL
-
 Escherichia coli
3.5.2.6 NDM
-
 Klebsiella pneumoniae
3.5.2.6 NDM
-
 Acinetobacter baumannii
3.5.2.6 NDM
-
 Escherichia coli
3.5.2.6 NDM-1
-
 Klebsiella pneumoniae
3.5.2.6 NDM-10
-
 Klebsiella pneumoniae
3.5.2.6 NDM-12
-
 Escherichia coli
3.5.2.6 NDM-2
-
 Acinetobacter baumannii
3.5.2.6 NDM-3
-
 Escherichia coli
3.5.2.6 NDM-4
-
 Escherichia coli
3.5.2.6 NDM-5
-
 Escherichia coli
3.5.2.6 NDM-6
-
 Escherichia coli
3.5.2.6 NDM-7
-
 Escherichia coli
3.5.2.6 NDM-8
-
 Escherichia coli
3.5.2.6 NDM-9
-
 Klebsiella pneumoniae
3.5.2.6 New Delhi MBL
-
 Klebsiella pneumoniae
3.5.2.6 New Delhi MBL
-
 Acinetobacter baumannii
3.5.2.6 New Delhi MBL
-
 Escherichia coli
3.5.2.6 New Delhi metallo-beta-lactamase
-
 Klebsiella pneumoniae
3.5.2.6 New Delhi metallo-beta-lactamase
-
 Acinetobacter baumannii
3.5.2.6 New Delhi metallo-beta-lactamase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 25
-
 assay at Klebsiella pneumoniae
3.5.2.6 25
-
 assay at Acinetobacter baumannii
3.5.2.6 25
-
 assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.6 55.1
-
 melting temperature of the purified NDM-1 variant Escherichia coli
3.5.2.6 59.5
-
 melting temperature of the purified NDM-1 variant Klebsiella pneumoniae
3.5.2.6 61.4
-
 melting temperature of the purified NDM-1 variant Escherichia coli
3.5.2.6 63.4
-
 melting temperature of the purified NDM-1 variant Escherichia coli
3.5.2.6 64.6
-
 melting temperature of the purified NDM-1 variant Escherichia coli
3.5.2.6 72.1
-
 melting temperature of the purified NDM-1 variant Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 2 8 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 3
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 5
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 6
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 9
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 10
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 14
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 15
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 15
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 16
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 18
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 18
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 23
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 24
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 25
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 29
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 30
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 33
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 38
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 44
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 45
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 49
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 53
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 54
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 63
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 65
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 75
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 75
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 127
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 142
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 170
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 194
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 226
-
 doripenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 238
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 252
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 267
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 273
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 301
-
 meropenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 332
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 348
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 355
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 362
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 408
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 413
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 447
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 477
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 583
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 600
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 724
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 743
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 757
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 900
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1032
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1375
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 7.2
-
 assay at Klebsiella pneumoniae
3.5.2.6 7.2
-
 assay at Acinetobacter baumannii
3.5.2.6 7.2
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.079
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.102
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.139
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 0.146
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli

General Information

EC Number General Information Comment Organism
3.5.2.6 additional information different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution. Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview Klebsiella pneumoniae
3.5.2.6 additional information different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution. Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview Acinetobacter baumannii
3.5.2.6 additional information different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution. Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview Escherichia coli
3.5.2.6 additional information different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution.Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview Escherichia coli
3.5.2.6 physiological function New Delhi MBL (NDM)-1 is a clinically significant metallo beta-lactamase Klebsiella pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 130
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 140
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 150
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 170
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 180
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 180
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 200
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 210
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 220
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 230
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 230
-
 cefoxitin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 260
-
 ceftazidime pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1200
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1500
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1600
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1700
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1900
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 1900
-
 doripenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 2000
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2200
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2300
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2300
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2600
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2600
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2600
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 2800
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2900
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 2900
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 3000
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 3100
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4100
-
 ampicillin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 4100
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4100
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4100
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4200
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4300
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4500
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4600
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 4900
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 5200
-
 meropenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 5400
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 5600
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 5800
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 5900
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 6000
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 6100
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 6100
-
 cefalotin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 6300
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 7400
-
 meropenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 7500
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 7600
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Klebsiella pneumoniae
3.5.2.6 8600
-
 doripenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 9200
-
 Imipenem pH 7.2, 25°C, recombinant enzyme Escherichia coli
3.5.2.6 10900
-
 nitrocefin pH 7.2, 25°C, recombinant enzyme Escherichia coli