show Characterization of a novel resistance-related deoxycytidine deaminase from Brassica oleracea var. capitata
Shibu, M.A.; Yang, H.H.; Lo, C.T.; Lin, H.S.; Liu, S.Y.; Peng, K.C.; J. Agric. Food Chem. 62, 1796-1801 (2014)
Data extracted from this reference:
Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.5
expressed in Escherichia coli
Brassica oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.0913
-
2'-deoxycytidine
at pH 7.5 and 27°C
Brassica oleracea
3.5.4.5
1.475
-
cytosine beta-D-arabinofuranoside
at pH 7.5 and 27°C
Brassica oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
20800
-
x * 20800, SDS-PAGE
Brassica oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
Brassica oleracea
-
2'-deoxyuridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Brassica oleracea
-
uridine + NH3
-
-
?
3.5.4.5
cytosine beta-D-arabinofuranoside + H2O
Brassica oleracea
-
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.5
Brassica oleracea
-
variant Brassica oleracea capitata
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.5
nickel chelating affinity column chromatography
Brassica oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
734018
Brassica oleracea
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
734018
Brassica oleracea
uridine + NH3
-
-
-
?
3.5.4.5
cytosine beta-D-arabinofuranoside + H2O
-
734018
Brassica oleracea
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.4.5
?
x * 20800, SDS-PAGE
Brassica oleracea
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.5
27
-
-
Brassica oleracea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
7.5
-
-
Brassica oleracea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
expressed in Escherichia coli
Brassica oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.4.5
0.0913
-
2'-deoxycytidine
at pH 7.5 and 27°C
Brassica oleracea
3.5.4.5
1.475
-
cytosine beta-D-arabinofuranoside
at pH 7.5 and 27°C
Brassica oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.4.5
20800
-
x * 20800, SDS-PAGE
Brassica oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
Brassica oleracea
-
2'-deoxyuridine + NH3
-
-
?
3.5.4.5
cytidine + H2O
Brassica oleracea
-
uridine + NH3
-
-
?
3.5.4.5
cytosine beta-D-arabinofuranoside + H2O
Brassica oleracea
-
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.5
nickel chelating affinity column chromatography
Brassica oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.5
2'-deoxycytidine + H2O
-
734018
Brassica oleracea
2'-deoxyuridine + NH3
-
-
-
?
3.5.4.5
cytidine + H2O
-
734018
Brassica oleracea
uridine + NH3
-
-
-
?
3.5.4.5
cytosine beta-D-arabinofuranoside + H2O
-
734018
Brassica oleracea
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.4.5
?
x * 20800, SDS-PAGE
Brassica oleracea
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.5.4.5
27
-
-
Brassica oleracea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.5
7.5
-
-
Brassica oleracea
General Information
EC Number
General Information
Commentary
Organism
3.5.4.5
physiological function
the enzyme plays an important role in the Trichoderma harzianum ETS 323 mediated resistance mechanism in young leaves of Brassice oleracea variant capitata during Rhizoctonia solani infection. The enzyme potentially neutralizes cytotoxic products of host lipoxygenase activity, thereby restricting the hypersensitivity-related programmed cell death induced in plants during the initial stages of infection
Brassica oleracea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.5.4.5
physiological function
the enzyme plays an important role in the Trichoderma harzianum ETS 323 mediated resistance mechanism in young leaves of Brassice oleracea variant capitata during Rhizoctonia solani infection. The enzyme potentially neutralizes cytotoxic products of host lipoxygenase activity, thereby restricting the hypersensitivity-related programmed cell death induced in plants during the initial stages of infection
Brassica oleracea