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Literature summary extracted from

  • Teixeira, M.; Carvalho, I.; Brodelius, M.
    omega-3 Fatty acid desaturase genes isolated from purslane (Portulaca oleracea L.): Expression in different tissues and response to cold and wound stress (2010), J. Agric. Food Chem., 58, 1870-1877.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.35 gene fad7, DNA and amino acid sequence determination and analysis Portulaca oleracea
1.14.19.35 gene fad8, DNA and amino acid sequence determination and analysis Portulaca oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.35 chloroplast membrane
-
 Portulaca oleracea 31969
-
1.14.19.35 additional information ubiquitous expression, tissue-specific levels Portulaca oleracea
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.35 a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Portulaca oleracea
-
 an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.35 Portulaca oleracea A8DTI8 gene fad7
-
1.14.19.35 Portulaca oleracea C6ZGG8 gene FAD8
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.35 leaf
-
 Portulaca oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.35 a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Portulaca oleracea an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.35 More three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence Portulaca oleracea

Synonyms

EC Number Synonyms Comment Organism
1.14.19.35 FAD7
-
 Portulaca oleracea
1.14.19.35 FAD8
-
 Portulaca oleracea
1.14.19.35 omega-3 fatty acid desaturase
-
 Portulaca oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.35 Ferredoxin
-
 Portulaca oleracea

Expression

EC Number Organism Comment Expression
1.14.19.35 Portulaca oleracea FAD7 is up-regulated by wounding but not by low temperature. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature up
1.14.19.35 Portulaca oleracea FAD8 is up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature up

General Information

EC Number General Information Comment Organism
1.14.19.35 evolution three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence Portulaca oleracea