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Literature summary extracted from

  • Lakshmi Bhargavi, P.; Prakasham, R.
    A fibrinolytic, alkaline and thermostable metalloprotease from the newly isolated Serratia sp RSPB11 (2013), Int. J. Biol. Macromol., 61, 479-486.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 EDTA strong inhibition with 2.6% residual activity at 10 mM, enzyme activity can be regained by the addition of Co2+, Cu2+, Fe2+, Mg2+, and Zn2+ ions after chelation of ions with EDTA Serratia marcescens
3.4.24.40 Fe2+ 68.66% residual activity at 5 mM Serratia marcescens
3.4.24.40 additional information not inhibited by iodoacetic acid and phenylmethylsulfonyl fluoride Serratia marcescens
3.4.24.40 Phenanthroline strong inhibition with 4.65% residual activity at 10 mM Serratia marcescens
3.4.24.40 Sn2+ 59.07% residual activity at 5 mM Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.40 Co2+ 120.1% activity at 5 mM Serratia marcescens
3.4.24.40 additional information not influenced by Ba2+, Ca2+, Cu2+, K+, Mg2+, Na+, and Ni2+ Serratia marcescens
3.4.24.40 Zn2+ 114.02% activity at 5 mM Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.40 50000
-
 x * 50000, SDS-PAGE Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.40 Fibrin + H2O Serratia marcescens
-
 ?
-
 ?
3.4.24.40 Fibrin + H2O Serratia marcescens RSPB11
-
 ?
-
 ?
3.4.24.40 Fibrinogen + H2O Serratia marcescens the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h ?
-
 ?
3.4.24.40 Fibrinogen + H2O Serratia marcescens RSPB11 the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h ?
-
 ?
3.4.24.40 additional information Serratia marcescens the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator ?
-
 ?
3.4.24.40 additional information Serratia marcescens RSPB11 the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Serratia marcescens
-
-
-
3.4.24.40 Serratia marcescens RSPB11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G-75 gel filtration Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.40 additional information
-
 the crude enzyme shows specific activity of 814.7 units/mg, the 15.2fold purified enzyme shows specific activity of 12365.9 units/mg. One unit of proteolytic enzyme activity is defined as the amount of enzyme that liberates 0.001 mg tyrosine per ml per minute from casein, at pH 7.5 and 37°C Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 casein + H2O Km of 1.261 mg/ml for casein Serratia marcescens ?
-
 ?
3.4.24.40 casein + H2O Km of 1.261 mg/ml for casein Serratia marcescens RSPB11 ?
-
 ?
3.4.24.40 Fibrin + H2O
-
 Serratia marcescens ?
-
 ?
3.4.24.40 Fibrin + H2O
-
 Serratia marcescens RSPB11 ?
-
 ?
3.4.24.40 Fibrinogen + H2O the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h Serratia marcescens ?
-
 ?
3.4.24.40 Fibrinogen + H2O the enzyme hydrolyzes the Aalpha chain in 1 min, the Bbeta chain in 5 min, and the gamma chain in more than 1 h Serratia marcescens RSPB11 ?
-
 ?
3.4.24.40 additional information the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator Serratia marcescens ?
-
 ?
3.4.24.40 additional information the enzyme does not degrade urokinase, streptokinase, and tissue plasminogen activator Serratia marcescens RSPB11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.40 ? x * 50000, SDS-PAGE Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.40 Serratia marcescens metalloproteinase
-
 Serratia marcescens
3.4.24.40 SlpB
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.40 37
-
-
 Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.40 30 50 more than 50% activity between 30 and 50°C Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 4 70 after 1 h of incubation, the enzyme shows 100% activity between 4 and 37°C, 95% activity at 40°C, 85% activity at 50°C, 65% activity at 60°C, and 50% activity at 70°C Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 9
-
-
 Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.40 7 11 more than 50% activity between pH 7.0 and 11.0 Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.40 6 10 the enzyme shows more than 50% activity at pH 6.0-10.0 after 1 h incubation time, while residual activities ranging from 43.20-8.30% are observed at higher pH (11.0-12.0) and lower pH (4.0-5.0) values Serratia marcescens