BRENDA - Enzyme Database

Purification and biochemical characterization of membrane-bound neutral ceramidase from camel brain (Camelus dromedarius)

Chathoth, S.; Thayyullathil, F.; Galadari, A.; Patel, M.; Galadari, S.; Int. J. Biochem. Mol. Biol. 4, 54-66 (2013)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.23
ADP
-
Camelus dromedarius
3.5.1.23
Cu2+
-
Camelus dromedarius
3.5.1.23
cysteine
-
Camelus dromedarius
3.5.1.23
GSH
-
Camelus dromedarius
3.5.1.23
GSSG
-
Camelus dromedarius
3.5.1.23
N-acetylcysteine
-
Camelus dromedarius
3.5.1.23
phosphatidic acid
complete inhibition
Camelus dromedarius
3.5.1.23
phosphatidylcholine
complete inhibition
Camelus dromedarius
3.5.1.23
phosphatidylserine
complete inhibition
Camelus dromedarius
3.5.1.23
TMP
-
Camelus dromedarius
3.5.1.23
UMP
-
Camelus dromedarius
3.5.1.23
Zn2+
-
Camelus dromedarius
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.23
additional information
-
additional information
Michaelis-Menten kinetics
Camelus dromedarius
3.5.1.23
0.0498
-
C12-NBD-Cer
pH 7.5, 37C
Camelus dromedarius
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.23
membrane
membrane-bound
Camelus dromedarius
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.23
Ca2+
activates
Camelus dromedarius
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.23
80000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
Camelus dromedarius
3.5.1.23
100000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
Camelus dromedarius
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.23
Camelus dromedarius
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.5.1.23
glycoprotein
deeglycosylation by glycosidase F treatment
Camelus dromedarius
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.23
native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography
Camelus dromedarius
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.23
brain
-
Camelus dromedarius
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.1.23
230.6
-
purified enzyme, pH 7.5, 37C
Camelus dromedarius
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.23
C12-NBD-Cer + H2O
the enzyme catalyzes also the reverse reaction of ceramide synthesis
733984
Camelus dromedarius
NBD-dodecanoic acid + D-erythro-sphingosine
-
-
-
r
3.5.1.23
N-hexanoyl-D-erythro-sphingosine + H2O
-
733984
Camelus dromedarius
hexanoate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-lauroyl-D-erythro-sphingosine + H2O
the enzyme catalyzes also the reverse reaction of ceramide synthesis
733984
Camelus dromedarius
laurate + D-erythro-sphingosine
-
-
-
r
3.5.1.23
N-lignoceroyl-D-erythro-sphingosine + H2O
low activity
733984
Camelus dromedarius
lignocerate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-myristoyl-D-erythro-sphingosine + H2O
best substrate
733984
Camelus dromedarius
myristate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-palmitoyl-D-erythro-sphingosine + H2O
-
733984
Camelus dromedarius
palmitate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-stearoyl-D-erythro-sphingosine + H2O
-
733984
Camelus dromedarius
stearate + D-erythro-sphingosine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.5.1.23
?
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
Camelus dromedarius
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.23
37
-
assay at
Camelus dromedarius
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.23
7
-
-
Camelus dromedarius
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.23
4
10
activity range, high activity at pH 6.0-8.0, profile overview
Camelus dromedarius
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.23
ADP
-
Camelus dromedarius
3.5.1.23
Cu2+
-
Camelus dromedarius
3.5.1.23
cysteine
-
Camelus dromedarius
3.5.1.23
GSH
-
Camelus dromedarius
3.5.1.23
GSSG
-
Camelus dromedarius
3.5.1.23
N-acetylcysteine
-
Camelus dromedarius
3.5.1.23
phosphatidic acid
complete inhibition
Camelus dromedarius
3.5.1.23
phosphatidylcholine
complete inhibition
Camelus dromedarius
3.5.1.23
phosphatidylserine
complete inhibition
Camelus dromedarius
3.5.1.23
TMP
-
Camelus dromedarius
3.5.1.23
UMP
-
Camelus dromedarius
3.5.1.23
Zn2+
-
Camelus dromedarius
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.23
additional information
-
additional information
Michaelis-Menten kinetics
Camelus dromedarius
3.5.1.23
0.0498
-
C12-NBD-Cer
pH 7.5, 37C
Camelus dromedarius
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.23
membrane
membrane-bound
Camelus dromedarius
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.23
Ca2+
activates
Camelus dromedarius
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.5.1.23
80000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
Camelus dromedarius
3.5.1.23
100000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
Camelus dromedarius
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.5.1.23
glycoprotein
deeglycosylation by glycosidase F treatment
Camelus dromedarius
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.23
native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography
Camelus dromedarius
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.23
brain
-
Camelus dromedarius
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.5.1.23
230.6
-
purified enzyme, pH 7.5, 37C
Camelus dromedarius
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.23
C12-NBD-Cer + H2O
the enzyme catalyzes also the reverse reaction of ceramide synthesis
733984
Camelus dromedarius
NBD-dodecanoic acid + D-erythro-sphingosine
-
-
-
r
3.5.1.23
N-hexanoyl-D-erythro-sphingosine + H2O
-
733984
Camelus dromedarius
hexanoate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-lauroyl-D-erythro-sphingosine + H2O
the enzyme catalyzes also the reverse reaction of ceramide synthesis
733984
Camelus dromedarius
laurate + D-erythro-sphingosine
-
-
-
r
3.5.1.23
N-lignoceroyl-D-erythro-sphingosine + H2O
low activity
733984
Camelus dromedarius
lignocerate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-myristoyl-D-erythro-sphingosine + H2O
best substrate
733984
Camelus dromedarius
myristate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-palmitoyl-D-erythro-sphingosine + H2O
-
733984
Camelus dromedarius
palmitate + D-erythro-sphingosine
-
-
-
?
3.5.1.23
N-stearoyl-D-erythro-sphingosine + H2O
-
733984
Camelus dromedarius
stearate + D-erythro-sphingosine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.5.1.23
?
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
Camelus dromedarius
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.5.1.23
37
-
assay at
Camelus dromedarius
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.23
7
-
-
Camelus dromedarius
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.23
4
10
activity range, high activity at pH 6.0-8.0, profile overview
Camelus dromedarius