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Literature summary extracted from

  • Chathoth, S.; Thayyullathil, F.; Galadari, A.; Patel, M.; Galadari, S.
    Purification and biochemical characterization of membrane-bound neutral ceramidase from camel brain (Camelus dromedarius) (2013), Int. J. Biochem. Mol. Biol., 4, 54-66.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 ADP
-
Camelus dromedarius
3.5.1.23 Cu2+
-
Camelus dromedarius
3.5.1.23 cysteine
-
Camelus dromedarius
3.5.1.23 GSH
-
Camelus dromedarius
3.5.1.23 GSSG
-
Camelus dromedarius
3.5.1.23 N-acetylcysteine
-
Camelus dromedarius
3.5.1.23 phosphatidic acid complete inhibition Camelus dromedarius
3.5.1.23 phosphatidylcholine complete inhibition Camelus dromedarius
3.5.1.23 phosphatidylserine complete inhibition Camelus dromedarius
3.5.1.23 TMP
-
Camelus dromedarius
3.5.1.23 UMP
-
Camelus dromedarius
3.5.1.23 Zn2+
-
Camelus dromedarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 additional information
-
additional information Michaelis-Menten kinetics Camelus dromedarius
3.5.1.23 0.0498
-
C12-NBD-Cer pH 7.5, 37°C Camelus dromedarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 membrane membrane-bound Camelus dromedarius 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 Ca2+ activates Camelus dromedarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.23 80000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE Camelus dromedarius
3.5.1.23 100000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE Camelus dromedarius

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Camelus dromedarius
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.23 glycoprotein deeglycosylation by glycosidase F treatment Camelus dromedarius

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23 native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography Camelus dromedarius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
Camelus dromedarius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.23 230.6
-
purified enzyme, pH 7.5, 37°C Camelus dromedarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 C12-NBD-Cer + H2O the enzyme catalyzes also the reverse reaction of ceramide synthesis Camelus dromedarius NBD-dodecanoic acid + D-erythro-sphingosine
-
r
3.5.1.23 N-hexanoyl-D-erythro-sphingosine + H2O
-
Camelus dromedarius hexanoate + D-erythro-sphingosine
-
?
3.5.1.23 N-lauroyl-D-erythro-sphingosine + H2O the enzyme catalyzes also the reverse reaction of ceramide synthesis Camelus dromedarius laurate + D-erythro-sphingosine
-
r
3.5.1.23 N-lignoceroyl-D-erythro-sphingosine + H2O low activity Camelus dromedarius lignocerate + D-erythro-sphingosine
-
?
3.5.1.23 N-myristoyl-D-erythro-sphingosine + H2O best substrate Camelus dromedarius myristate + D-erythro-sphingosine
-
?
3.5.1.23 N-palmitoyl-D-erythro-sphingosine + H2O
-
Camelus dromedarius palmitate + D-erythro-sphingosine
-
?
3.5.1.23 N-stearoyl-D-erythro-sphingosine + H2O
-
Camelus dromedarius stearate + D-erythro-sphingosine
-
?

Subunits

EC Number Subunits Comment Organism
3.5.1.23 ? x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE Camelus dromedarius

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 CBCDase
-
Camelus dromedarius
3.5.1.23 nCDase
-
Camelus dromedarius
3.5.1.23 neutral ceramidase
-
Camelus dromedarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.23 37
-
assay at Camelus dromedarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 7
-
-
Camelus dromedarius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 4 10 activity range, high activity at pH 6.0-8.0, profile overview Camelus dromedarius