EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.23 | ADP | - |
Camelus dromedarius | |
3.5.1.23 | Cu2+ | - |
Camelus dromedarius | |
3.5.1.23 | cysteine | - |
Camelus dromedarius | |
3.5.1.23 | GSH | - |
Camelus dromedarius | |
3.5.1.23 | GSSG | - |
Camelus dromedarius | |
3.5.1.23 | N-acetylcysteine | - |
Camelus dromedarius | |
3.5.1.23 | phosphatidic acid | complete inhibition | Camelus dromedarius | |
3.5.1.23 | phosphatidylcholine | complete inhibition | Camelus dromedarius | |
3.5.1.23 | phosphatidylserine | complete inhibition | Camelus dromedarius | |
3.5.1.23 | TMP | - |
Camelus dromedarius | |
3.5.1.23 | UMP | - |
Camelus dromedarius | |
3.5.1.23 | Zn2+ | - |
Camelus dromedarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.23 | additional information | - |
additional information | Michaelis-Menten kinetics | Camelus dromedarius | |
3.5.1.23 | 0.0498 | - |
C12-NBD-Cer | pH 7.5, 37°C | Camelus dromedarius |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.23 | membrane | membrane-bound | Camelus dromedarius | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.23 | Ca2+ | activates | Camelus dromedarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 80000 | - |
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
3.5.1.23 | 100000 | - |
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.23 | Camelus dromedarius | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.5.1.23 | glycoprotein | deeglycosylation by glycosidase F treatment | Camelus dromedarius |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.23 | native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography | Camelus dromedarius |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.23 | brain | - |
Camelus dromedarius | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 230.6 | - |
purified enzyme, pH 7.5, 37°C | Camelus dromedarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.23 | C12-NBD-Cer + H2O | the enzyme catalyzes also the reverse reaction of ceramide synthesis | Camelus dromedarius | NBD-dodecanoic acid + D-erythro-sphingosine | - |
r | |
3.5.1.23 | N-hexanoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | hexanoate + D-erythro-sphingosine | - |
? | |
3.5.1.23 | N-lauroyl-D-erythro-sphingosine + H2O | the enzyme catalyzes also the reverse reaction of ceramide synthesis | Camelus dromedarius | laurate + D-erythro-sphingosine | - |
r | |
3.5.1.23 | N-lignoceroyl-D-erythro-sphingosine + H2O | low activity | Camelus dromedarius | lignocerate + D-erythro-sphingosine | - |
? | |
3.5.1.23 | N-myristoyl-D-erythro-sphingosine + H2O | best substrate | Camelus dromedarius | myristate + D-erythro-sphingosine | - |
? | |
3.5.1.23 | N-palmitoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | palmitate + D-erythro-sphingosine | - |
? | |
3.5.1.23 | N-stearoyl-D-erythro-sphingosine + H2O | - |
Camelus dromedarius | stearate + D-erythro-sphingosine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.23 | ? | x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE | Camelus dromedarius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.23 | CBCDase | - |
Camelus dromedarius |
3.5.1.23 | nCDase | - |
Camelus dromedarius |
3.5.1.23 | neutral ceramidase | - |
Camelus dromedarius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 37 | - |
assay at | Camelus dromedarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 7 | - |
- |
Camelus dromedarius |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.23 | 4 | 10 | activity range, high activity at pH 6.0-8.0, profile overview | Camelus dromedarius |