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Literature summary extracted from

  • Song, M.; Kim, A.C.; Gorzalski, A.J.; MacLean, M.; Young, S.; Ginzel, M.D.; Blomquist, G.J.; Tittiger, C.
    Functional characterization of myrcene hydroxylases from two geographically distinct Ips pini populations (2013), Insect Biochem. Mol. Biol., 43, 336-343.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.31 expressed in Sf9 cells Ips pini

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.31 61000
-
 x * 61000, calculated from amino acid sequence Ips pini

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.31 myrcene + NADPH + H+ + O2 Ips pini 87% yield of (-)-ipsdienol, 13% yield of (+)-ipsdienol ipsdienol + NADP+ + H2O
-
 ?
1.14.14.31 myrcene + NADPH + H+ + O2 Ips pini the enzyme strongly prefers myrcene over other substrates ipsdienol + NADP+ + H2O 80% yield of (-)-ipsdienol, 20% yield of (+)-ipsdienol ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.31 Ips pini M1JEK6
-
-
1.14.14.31 Ips pini Q00G65
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.31 midgut
-
 Ips pini
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.31 (+)-alpha-pinene + NADPH + H+ + O2
-
 Ips pini verbenol + NADP+ + H2O
-
 ?
1.14.14.31 (-)-alpha-pinene + NADPH + H+ + O2
-
 Ips pini cis-verbenol + NADP+ + H2O
-
 ?
1.14.14.31 (-)-alpha-pinene + NADPH + H+ + O2
-
 Ips pini myrtenol + NADP+ + H2O
-
 ?
1.14.14.31 3-carene + NADPH + H+ + O2
-
 Ips pini ?
-
 ?
1.14.14.31 additional information no activity with alpha-phellandrene, (-)-beta-pinene, gamma-terpinene, or terpinolene Ips pini ?
-
 ?
1.14.14.31 myrcene + NADPH + H+ + O2 87% yield of (-)-ipsdienol, 13% yield of (+)-ipsdienol Ips pini ipsdienol + NADP+ + H2O
-
 ?
1.14.14.31 myrcene + NADPH + H+ + O2 the enzyme strongly prefers myrcene over other substrates Ips pini ipsdienol + NADP+ + H2O 80% yield of (-)-ipsdienol, 20% yield of (+)-ipsdienol ?
1.14.14.31 R-(+)-limonene + NADPH + H+ + O2
-
 Ips pini ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.31 ? x * 61000, calculated from amino acid sequence Ips pini

Synonyms

EC Number Synonyms Comment Organism
1.14.14.31 CYP9T2
-
 Ips pini
1.14.14.31 CYP9T3
-
 Ips pini
1.14.14.31 myrcene hydroxylase
-
 Ips pini

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.31 NADPH
-
 Ips pini

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.31 Ips pini calculated from amino acid sequence
-
 9

Expression

EC Number Organism Comment Expression
1.14.14.31 Ips pini CYP9T2 mRNA levels are not induced in adults exposed to myrcene-saturated atmosphere additional information