EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.3 | ssDNA | activates the ATPase activity of the enzyme | Plasmodium falciparum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.6.2.3 | expression of recombinant His-tagged enzyme PfRuvB1 in Escherichia coli strain BL21(DE3) and BL21 codon plus in inclusion bodies, a heat shock protocol helps to obtain the PfRuvB1 expression in the soluble fraction | Plasmodium falciparum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.6.2.3 | additional information | the enzyme PfRuvB1 localizes mainly to the nucleus | Plasmodium falciparum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.3 | Mg2+ | required | Plasmodium falciparum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.6.2.3 | 59000 | - |
x * 59000, about, sequence calculation | Plasmodium falciparum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.3 | ATP + H2O | Plasmodium falciparum | - |
ADP + phosphate | - |
? | |
5.6.2.3 | additional information | Plasmodium falciparum | enzyme RuvB1 is an active DNA helicase and translocates in the 5'-3' direction | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.2.3 | Plasmodium falciparum | J7HDV0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.2.3 | recombinant His-tagged enzyme PfRuvB1 from Escherichia coli strain BL21(DE3) and BL21 codon plus soluble fraction by nickel affinity chromatography to homogeneity | Plasmodium falciparum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.6.2.3 | additional information | the enzyme PfRuvB1 is constitutively expressed during all the stages of intraerythrocytic cycle of Plasmodium. falciparum | Plasmodium falciparum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.3 | ATP + H2O | - |
Plasmodium falciparum | ADP + phosphate | - |
? | |
5.6.2.3 | ATP + H2O | PfRuvB1 is an ATPase and this activity increases significantly in the presence of ssDNA, PfRuvB1 also contains DNA helicase activity and translocates preferentially in 5' to 3' direction, substrates are M13 mp19 ssDNA and oligonucleotides | Plasmodium falciparum | ADP + phosphate | - |
? | |
5.6.2.3 | additional information | enzyme RuvB1 is an active DNA helicase and translocates in the 5'-3' direction | Plasmodium falciparum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.6.2.3 | ? | x * 59000, about, sequence calculation | Plasmodium falciparum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.2.3 | RuvB1 | - |
Plasmodium falciparum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.2.3 | 37 | - |
assay at | Plasmodium falciparum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.2.3 | 8 | - |
assay at | Plasmodium falciparum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.6.2.3 | evolution | the RuvB family of protein contains two similar kinds of proteins i.e. RuvB1 and RuvB2 from yeast to human. Plasmodium falciparum contains three RuvB proteins (PfRuvB1, PfRuvB2 and PfRuvB3) instead of two. These proteins belong to the AAA+ class of proteins and are critical components of several multiprotein complexes involved in diverse cellular activities. PfRuvB1 shows considerable homology with human as well as yeast RuvB1 and contains Walker motif A and Walker motif B. | Plasmodium falciparum |