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Literature summary extracted from

  • Bello, M.; Barrera-Perez, V.; Morin, D.; Epstein, L.
    The Neurospora crassa mutant NcDELTAEgt-1 identifies an ergothioneine biosynthetic gene and demonstrates that ergothioneine enhances conidial survival and protects against peroxide toxicity during conidial germination (2012), Fungal Genet. Biol., 49, 160-172.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.10 additional information the NcDELTAEgt-1 enzyme knockout strain does not produce ergothioneine. The mutant strain is not pleiotropically affected in rate of hyphal elongation in Vogel's medium either with or without ammonium nitrate and in the rate of germination of macroconidia on Vogel's medium, the mutant is also not differently affected from the wild-type by menadione and cupric sulfate, but germination of NcDELTAEgt-1 conidia is significantly more sensitive to tert-butyl hydroperoxide than the wild-type, phenotype, overview Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.10 hercynine + L-cysteine + O2 Neurospora crassa
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.21.3.10 hercynine + L-cysteine + O2 Neurospora crassa ATCC 24698
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.10 Neurospora crassa Q7RX33 gene egt1 or NCU04343
-
1.21.3.10 Neurospora crassa ATCC 24698 Q7RX33 gene egt1 or NCU04343
-
1.21.3.10 no activity in Bremia lactucae
-
-
-
1.21.3.10 no activity in Candida albicans
-
-
-
1.21.3.10 no activity in Pichia ssp.
-
-
-
1.21.3.10 no activity in Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.21.3.10 conidium
-
 Neurospora crassa
-
1.21.3.10 mycelium
-
 Neurospora crassa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.10 hercynine + L-cysteine + O2
-
 Neurospora crassa S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.21.3.10 hercynine + L-cysteine + O2
-
 Neurospora crassa ATCC 24698 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.3.10 Egt1
-
 Neurospora crassa

General Information

EC Number General Information Comment Organism
1.21.3.10 evolution distribution of NcEgt-1 homologues in fungi, overview. The enzyme homologues are present in all of the true fungal groups in which ergothioneine is identified: Mucoromycotina (i.e., Mucorales), Agaricomycotina (i.e., Agaricales, Boletales, and Cantharellales), Pucciniomycotina (i.e., Sporidiobolales), and the Pezizomycotina (i.e., Eurotiales, Glomerellales, Hypocreales, and Sordariales) Neurospora crassa
1.21.3.10 malfunction no ergothioneine is formed in the egt1-knockout mutant strain of Neurospora crassa, the mutant conidia show higher sensitivity to tert-butyl hydroperoxide compared to the wild-type strain, phenotype, overview Neurospora crassa
1.21.3.10 metabolism the enzyme catalyzes a step in the ergothioneine, EGT, biosynthetic pathway Neurospora crassa
1.21.3.10 physiological function the enzyme is essentially involved in ergothioneine biosynthesis, which enhances conidial survival and protects against peroxide toxicity during conidial germination, overview. Ergothioneine does not have discernible pleiotropic affects and does not appear to protect against either Cu2+ toxicity or superoxide in vivo Neurospora crassa