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Literature summary extracted from

  • Miranda, M.; Galli, L.M.; Enriquez, M.; Szabo, L.A.; Gao, X.; Hannoush, R.N.; Burrus, L.W.
    Identification of the WNT1 residues required for palmitoylation by Porcupine (2014), FEBS Lett., 588, 4815-4824.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.250 additional information substrate glycosylation promotes, but is not absolutely required for Wnt1 palmitoylation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.250 membrane bound Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Homo sapiens
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.250 Homo sapiens Q9H237
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.250 HEK-293T cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine chick WNT1 protein, the enzyme is an O-acyl transferase for WNT1 protein, WNT1 residues 214-234 are sufficient for PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but not Cys, is tolerated in palmitoylation and biological assays. Transiently transfection of HEK-293T cells with WNT1. WNT1 mutant S224T is accepted as substrate, while mutants S224A and S224C are inactive Homo sapiens CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 additional information generation of a fusion protein that is palmitoylated in a PORCN-dependent manner, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.250 PORCN
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.250 evolution the enzyme is a member of the membrane-bound O-acyl transferase (MBOAT) superfamily Homo sapiens
2.3.1.250 physiological function the enzyme is enzyme responsible for palmitoylating Wnt proteins, importance of enzyme catalyzed palmitoylation for WNT1 activity and Wnt signaling Homo sapiens