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Literature summary extracted from

  • Sasaki, M.; Takegawa, K.; Kimura, Y.
    Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus (2014), FEBS Lett., 588, 3395-3402.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.17 EDTA 5 mM, 13.2% residual activity Myxococcus xanthus
3.6.1.17 glycerol phosphate 20 mM, 46.1% residual activity Myxococcus xanthus
3.6.1.17 iodoacetic acid 10 mM, 58.8% residual activity Myxococcus xanthus
3.6.1.17 N-ethylmaleimide 1 mM, 73.8% residual activity Myxococcus xanthus
3.6.1.17 trisodium phosphate 20 mM, 24.8% residual activity Myxococcus xanthus
3.6.1.41 EDTA 5 mM, 13.2% residual activity; 5 mM, 2.8% residual activity Myxococcus xanthus
3.6.1.41 glycerol phosphate 20 mM, 33.4% residual activity; 20 mM, 46.1% residual activity Myxococcus xanthus
3.6.1.41 iodoacetic acid 10 mM, 58.8% residual activity Myxococcus xanthus
3.6.1.41 N-ethylmaleimide 1 mM, 62.6% residual activity; 1 mM, 73.8% residual activity Myxococcus xanthus
3.6.1.41 sodium orthovanadate 1 mM, 6.7% residual activity Myxococcus xanthus
3.6.1.41 trisodium phosphate 20 mM, 24.8% residual activity; 20 mM, 6.2% residual activity Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.17 0.14
-
P1,P4-bis(5'-adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.17 0.19
-
P1,P5-bis(5'-adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.14
-
P1,P4-bis(adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.19
-
P1,P5-bis(adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.49
-
P1,P5-bis(adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Co2+ Myxococcus xanthus
3.6.1.41 0.86
-
P1,P4-bis(adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Co2+ Myxococcus xanthus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.17 Co2+ about 50% of the activity with Mn2+. In the presence of Mn2+, PrpA hydrolyzes P1,P3-bis(5'-adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+, PrpA hydrolyzes P1,P3-bis(5'-adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 Myxococcus xanthus
3.6.1.17 Mn2+ best activator. In the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P3-bis(5'-adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 Myxococcus xanthus
3.6.1.41 Co2+ about 50% of the activity with Mn2+. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 Myxococcus xanthus
3.6.1.41 Co2+ required for ApnA hydrolysis Myxococcus xanthus
3.6.1.41 Mn2+ best activator. In the presence of Mn2+, PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes P1,P5-bis(adenosyl)tetraphosphate into two molecules of ADP, reaction of EC 3.6.1.17 Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.17 Myxococcus xanthus Q1DC62
-
-
3.6.1.17 Myxococcus xanthus DK 1622 Q1DC62
-
-
3.6.1.41 Myxococcus xanthus Q1CWE7
-
-
3.6.1.41 Myxococcus xanthus Q1DC62
-
-
3.6.1.41 Myxococcus xanthus DK 1622 Q1CWE7
-
-
3.6.1.41 Myxococcus xanthus DK 1622 Q1DC62
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.17 additional information PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP Myxococcus xanthus ?
-
?
3.6.1.17 additional information PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP Myxococcus xanthus DK 1622 ?
-
?
3.6.1.17 P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
Myxococcus xanthus AMP + ATP in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes Ap4A into two molecules of ADP, reaction of EC 3.6.1.41 ?
3.6.1.17 P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
Myxococcus xanthus DK 1622 AMP + ATP in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, whereas in the presence of Co2+ PrpA hydrolyzes Ap4A into two molecules of ADP, reaction of EC 3.6.1.41 ?
3.6.1.17 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
-
Myxococcus xanthus AMP + adenosine 5'-tetraphosphate
-
?
3.6.1.17 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
-
Myxococcus xanthus DK 1622 AMP + adenosine 5'-tetraphosphate
-
?
3.6.1.41 additional information enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP Myxococcus xanthus ?
-
?
3.6.1.41 additional information PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP Myxococcus xanthus ?
-
?
3.6.1.41 additional information enzyme exhibits high phosphatase activity towards 4-nitrophenyl phospahte, and hydrolase activity towards ApnA and ATP Myxococcus xanthus DK 1622 ?
-
?
3.6.1.41 additional information PrpA exhibits a phosphatase activity towards p-nitrophenyl phosphate, tyrosine phosphopeptide and tyrosine-phosphorylated protein and a weak hydrolase activity towards ATP Myxococcus xanthus DK 1622 ?
-
?
3.6.1.41 P1,P4-bis(adenosyl)tetraphosphate + H2O
-
Myxococcus xanthus 2 ADP
-
?
3.6.1.41 P1,P4-bis(adenosyl)tetraphosphate + H2O
-
Myxococcus xanthus 2 ADP in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP ?
3.6.1.41 P1,P4-bis(adenosyl)tetraphosphate + H2O
-
Myxococcus xanthus DK 1622 2 ADP
-
?
3.6.1.41 P1,P4-bis(adenosyl)tetraphosphate + H2O
-
Myxococcus xanthus DK 1622 2 ADP in the presence of Mn2+, PrpA hydrolyzes Ap4A mainly into AMP and ATP, reaction of EC 3.6.1.17, whereas in the presence of Co2+, PrpA hydrolyzes Ap4A into two molecules of ADP ?
3.6.1.41 P1,P5-bis(adenosyl)pentaphosphate + H2O
-
Myxococcus xanthus ADP + ATP
-
?
3.6.1.41 P1,P5-bis(adenosyl)pentaphosphate + H2O
-
Myxococcus xanthus DK 1622 ADP + ATP
-
?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.17 MXAN_1509
-
Myxococcus xanthus
3.6.1.17 PrpA
-
Myxococcus xanthus
3.6.1.41 APAH
-
Myxococcus xanthus
3.6.1.41 MXAN_1509
-
Myxococcus xanthus
3.6.1.41 MXAN_7163
-
Myxococcus xanthus
3.6.1.41 PrpA
-
Myxococcus xanthus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.17 0.035
-
P1,P5-bis(5'-adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.17 0.055
-
P1,P4-bis(5'-adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.035
-
P1,P5-bis(adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.055
-
P1,P4-bis(adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 7.12
-
P1,P4-bis(adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Co2+ Myxococcus xanthus
3.6.1.41 24
-
P1,P5-bis(adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Co2+ Myxococcus xanthus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.17 0.18
-
P1,P5-bis(5'-adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.17 0.4
-
P1,P4-bis(5'-adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.18
-
P1,P5-bis(adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 0.4
-
P1,P4-bis(adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Mn2+ Myxococcus xanthus
3.6.1.41 8.28
-
P1,P4-bis(adenosyl)tetraphosphate pH 8.0, 50°C, presence of 1 mM Co2+ Myxococcus xanthus
3.6.1.41 48.57
-
P1,P5-bis(adenosyl)pentaphosphate pH 8.0, 50°C, presence of 1 mM Co2+ Myxococcus xanthus