BRENDA - Enzyme Database

Site-directed mutagenesis of histidine residues in the DELTA12 acyl-lipid desaturase of Synechocystis

Avelange-Macherel M.-H.; Macherel D.; Wada H.; Murata N.; FEBS Lett. 361, 111-114 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.6
expressed in Escherichia coli BL21(DE3) cells
Synechocystis sp.
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.6
H109R
inactive
Synechocystis sp.
1.14.19.6
H129R
inactive
Synechocystis sp.
1.14.19.6
H287R
inactive
Synechocystis sp.
1.14.19.6
H290R
inactive
Synechocystis sp.
1.14.19.6
H90R
inactive
Synechocystis sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.6
36000
-
x * 36000, SDS-PAGE
Synechocystis sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.6
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechocystis sp.
-
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.6
Synechocystis sp.
P20388
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.6
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733846
Synechocystis sp.
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.6
?
x * 36000, SDS-PAGE
Synechocystis sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.6
Ferredoxin
-
Synechocystis sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.6
expressed in Escherichia coli BL21(DE3) cells
Synechocystis sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.6
Ferredoxin
-
Synechocystis sp.
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.6
H109R
inactive
Synechocystis sp.
1.14.19.6
H129R
inactive
Synechocystis sp.
1.14.19.6
H287R
inactive
Synechocystis sp.
1.14.19.6
H290R
inactive
Synechocystis sp.
1.14.19.6
H90R
inactive
Synechocystis sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.19.6
36000
-
x * 36000, SDS-PAGE
Synechocystis sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.6
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechocystis sp.
-
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.6
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
733846
Synechocystis sp.
1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.6
?
x * 36000, SDS-PAGE
Synechocystis sp.