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Literature summary extracted from

  • Zhang, H.; Zhang, Z.; Yang, J.; He, Z.G.
    Functional characterization of DnaB helicase and its modulation by single-stranded DNA binding protein in Mycobacterium tuberculosis (2014), FEBS J., 281, 1256-1266.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.3 single-stranded binding protein MtbSSB, physical interaction between single-stranded binding protein of Mycobacterium tuberculosis, MtbSSB, and MtbDnaB. The helicase activity of MtbDnaB is stimulated by MtbSSB at low concentrations and inhibited at high concentrations. An MtbSSB mutant with decreased binding affinity for ssDNA can stimulate the helicase activity of MtbDnaB over a wider concentration range than wild-type MtbSSB suggesting that MtbSSB assists in the loading of MtbDnaB on the DNA replication fork Mycobacterium tuberculosis
5.6.2.3 ssDNA the ATPase activity of enzyme DnaB is stimulated in the presence of ssDNA Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.3 gene dnaB, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlus (DE3)-RIL Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.3 additional information construction of an intein-deleted form of DnaB Mycobacterium tuberculosis
5.6.2.3 R227C site-directed mutagenesis, mutation in the ATP binding site ARPGVGKS Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.3 single-stranded binding protein MtbSSB, physical interaction between single-stranded binding protein of Mycobacterium tuberculosis, MtbSSB, and MtbDnaB. The helicase activity of MtbDnaB is stimulated by MtbSSB at low concentrations and inhibited at high concentrations. ssDNA-stimulated ATPase activity of DnaB can be inhibited in the presence of SSB, possibly due to the inability of DnaB to bind to the SSB-bound ssDNA Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.3 ATP + H2O Mycobacterium tuberculosis
-
 ADP + phosphate
-
 ?
5.6.2.3 additional information Mycobacterium tuberculosis the enzyme DnaB exhibits strong 5' to 3' helicase and ATPase activities ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.3 Mycobacterium tuberculosis
-
 gene dnaB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.3 recombinant His-tagged wild-type and intein-deleted mutant enzymes fromn Escherichia coli strain BL21-CodonPlus (DE3)-RIL by nicke affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.3 ATP + H2O
-
 Mycobacterium tuberculosis ADP + phosphate
-
 ?
5.6.2.3 additional information the enzyme DnaB exhibits strong 5' to 3' helicase and ATPase activities Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.3 DnaB helicase
-
 Mycobacterium tuberculosis
5.6.2.3 MtbDnaB
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
5.6.2.3 additional information an MtbSSB mutant with decreased binding affinity for ssDNA can stimulate the helicase activity of MtbDnaB over a wider concentration range than wild-type MtbSSB suggesting that MtbSSB assists in the loading of MtbDnaB on the DNA replication fork Mycobacterium tuberculosis
5.6.2.3 physiological function helicase DnaB is important in the initiation and extension stages of DNA replication. DnaB proteins also exhibit ATPase activity, which provides the energy necessary for their helicase activity, the helicase activity of MtbDnaB has 5' to 3 polarity. The ATPase activity of DnaB is stimulated in the presence of ssDNA Mycobacterium tuberculosis