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Literature summary extracted from

  • Gharib, G.; Rashid, N.; Bashir, Q.; Gardner, Q.T.; Akhtar, M.; Imanaka, T.
    Pcal_1699, an extremely thermostable malate dehydrogenase from hyperthermophilic archaeon Pyrobaculum calidifontis (2016), Extremophiles, 20, 57-67.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 expression in Escherichia coli Pyrobaculum calidifontis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 guanidine hydrochloride in the presence of 4 M guanidine hydrochloride enzyme structure is unfolded with complete loss of enzyme activity Pyrobaculum calidifontis
1.1.1.37 additional information urea cannot induce complete unfolding and inactivation of Pcal_1699 even at a final concentration of 8 M. Not inhibitory: EDTA Triton X-100, TWeen-20, dithiothreitol, glycerol Pyrobaculum calidifontis
1.1.1.37 sodium dodecylsulfate 0.1%, 70% loss of activity, 1%, complete loss of activity Pyrobaculum calidifontis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.23
-
 oxaloacetate pH 10, 55°C Pyrobaculum calidifontis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.37 additional information enzyme is not dependent on any metal ions Pyrobaculum calidifontis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 33292
-
 4 * 33292, calculated, 4 * 35086, MALDI-TOF Pyrobaculum calidifontis
1.1.1.37 35086
-
 4 * 33292, calculated, 4 * 35086, MALDI-TOF Pyrobaculum calidifontis
1.1.1.37 140000
-
 gel filtration Pyrobaculum calidifontis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Pyrobaculum calidifontis A3MWU9
-
-
1.1.1.37 Pyrobaculum calidifontis JCM 11548 A3MWU9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 oxaloacetate + NADH + H+
-
 Pyrobaculum calidifontis (S)-malate + NAD+
-
 ?
1.1.1.37 oxaloacetate + NADH + H+
-
 Pyrobaculum calidifontis JCM 11548 (S)-malate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 tetramer 4 * 33292, calculated, 4 * 35086, MALDI-TOF Pyrobaculum calidifontis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 Pcal_1699
-
 Pyrobaculum calidifontis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 90
-
-
 Pyrobaculum calidifontis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 98
-
 more than 90% residual activity after 6 h in boiling water. No significant change in CD spectra up to 100°C Pyrobaculum calidifontis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.37 77
-
 oxaloacetate pH 10, 55°C Pyrobaculum calidifontis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 10
-
 carbonate-bicarbonate buffer Pyrobaculum calidifontis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 additional information no activity with NADPH Pyrobaculum calidifontis
1.1.1.37 NADH
-
 Pyrobaculum calidifontis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.37 Pyrobaculum calidifontis calculated
-
 6.9