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Literature summary extracted from

  • Rajaei, S.; Noghabi, K.A.; Sadeghizadeh, M.; Zahiri, H.S.
    Characterization of a pH and detergent-tolerant, cold-adapted type I pullulanase from Exiguobacterium sp. SH3 (2015), Extremophiles, 19, 1145-1155.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.41 2-mercaptoethanol 1% v/v, 149% of initial activity Exiguobacterium sp.
3.2.1.41 dithiothreitol 0.2% w/v, 147% of initial activity Exiguobacterium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expression in Escherichia coli and Bacillus subtilis Exiguobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Cu2+ 2 mM, 79% residual activity Exiguobacterium sp.
3.2.1.41 additional information enzyme retains 99, 89, and 54% of its activity at 10% concentration of Triton-X100, Tween 20, and SDS, respectively. The enzyme retains 80.4 and 93.7% activity in the presence of commercial detergents Rika (7.5% v/v) and Fadisheh (2.5% w/v), respectively Exiguobacterium sp.
3.2.1.41 Zn2+ 2 mM, 48% residual activity Exiguobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 additional information
-
 pullulan Km value is 2.8 mg/ml, pH 8.5, 45°C Exiguobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Co2+ 2 mM, 113% of initial activity Exiguobacterium sp.
3.2.1.41 Mn2+ 2 mM, 143% of initial activity Exiguobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 110000
-
 x * 110000, SDS-PAGE Exiguobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Exiguobacterium sp. A0A0B5JT51
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 additional information negligible activity on wheat flour and maltodextrin, no activity on amylose Exiguobacterium sp. ?
-
 ?
3.2.1.41 potato starch + H2O 14% of the activity with pullulan Exiguobacterium sp. ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Exiguobacterium sp. ?
-
 ?
3.2.1.41 rice flour + H2O 15% of the activity with pullulan Exiguobacterium sp. ?
-
 ?
3.2.1.41 soluble starch + H2O 17% of the activity with pullulan Exiguobacterium sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 110000, SDS-PAGE Exiguobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 45
-
-
 Exiguobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 52
-
 melting temperature Exiguobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 37
-
 pullulan pH 8.5, 45°C Exiguobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 8.5
-
-
 Exiguobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 5 11 more than 60% of maximum activity Exiguobacterium sp.