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Literature summary extracted from

  • Ahmann, K.; Heilmann, M.; Feussner, I.
    Identification of a DELTA4-desaturase from the microalga Ostreococcus lucimarinus (2011), Eur. J. Lipid Sci. Technol., 113, 832-840.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.31 gene Egd4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme Euglena gracilis
1.14.19.31 gene Old4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme, GFP-Old4p does not form aggregates in yeast cells and localizes to the endoplasmic reticulum Ostreococcus sp. 'lucimarinus'

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.31 endoplasmic reticulum membrane
-
Ostreococcus sp. 'lucimarinus' 5789
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1.14.19.31 endoplasmic reticulum membrane
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Euglena gracilis 5789
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.31 additional information Ostreococcus sp. 'lucimarinus' the enzyme catalyzes the production of docosahexaenoic acid ?
-
?
1.14.19.31 additional information Ostreococcus sp. 'lucimarinus' CCE9901 the enzyme catalyzes the production of docosahexaenoic acid ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.31 Euglena gracilis Q6WNG7 gene Egd4
-
1.14.19.31 Euglena gracilis 1224-5/3 Q6WNG7 gene Egd4
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1.14.19.31 Ostreococcus sp. 'lucimarinus' A4RQJ7 gene Old4
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1.14.19.31 Ostreococcus sp. 'lucimarinus' CCE9901 A4RQJ7 gene Old4
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.31 additional information the enzyme catalyzes the production of docosahexaenoic acid Ostreococcus sp. 'lucimarinus' ?
-
?
1.14.19.31 additional information substrate specificity, overview. The recombinant enzyme from Euglena gracilis expressed in Saccharomyces cerevisiae strain INVSc1 shows strongest activity of 15% toward 16:3(n-3) and desaturated 22:5(n-3) and 22:4(n-6) with efficiencies of 12 and 4%, respectively. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates Euglena gracilis ?
-
?
1.14.19.31 additional information substrate specificity, the enzyme prefers very long chain polyunsaturated fatty acids, overview. The preferred substrate of the recombinant enzyme from Ostreococcus lucimarinus expressed in Saccharomyces cerevisiae strain INVSc1 with a desaturation activity of about 10% is 22:5(n-3) followed by 22:4(n-6) with an efficiency of about 4%. 16:3(n-3) is desaturated very poorly with an efficiency of about 1%. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates Ostreococcus sp. 'lucimarinus' ?
-
?
1.14.19.31 additional information substrate specificity, overview. The recombinant enzyme from Euglena gracilis expressed in Saccharomyces cerevisiae strain INVSc1 shows strongest activity of 15% toward 16:3(n-3) and desaturated 22:5(n-3) and 22:4(n-6) with efficiencies of 12 and 4%, respectively. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates Euglena gracilis 1224-5/3 ?
-
?
1.14.19.31 additional information the enzyme catalyzes the production of docosahexaenoic acid Ostreococcus sp. 'lucimarinus' CCE9901 ?
-
?
1.14.19.31 additional information substrate specificity, the enzyme prefers very long chain polyunsaturated fatty acids, overview. The preferred substrate of the recombinant enzyme from Ostreococcus lucimarinus expressed in Saccharomyces cerevisiae strain INVSc1 with a desaturation activity of about 10% is 22:5(n-3) followed by 22:4(n-6) with an efficiency of about 4%. 16:3(n-3) is desaturated very poorly with an efficiency of about 1%. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates Ostreococcus sp. 'lucimarinus' CCE9901 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.31 DELTA4-desaturase
-
Ostreococcus sp. 'lucimarinus'
1.14.19.31 DELTA4-desaturase
-
Euglena gracilis
1.14.19.31 DELTA4-fatty acid desaturase
-
Ostreococcus sp. 'lucimarinus'
1.14.19.31 DELTA4-fatty acid desaturase
-
Euglena gracilis
1.14.19.31 Egd4p
-
Euglena gracilis
1.14.19.31 Old4p
-
Ostreococcus sp. 'lucimarinus'

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.31 cytochrome b5 the enzyme exhibits a cytochrome b5 domain fused to its amino terminus and three histidine boxes that are typically found in front-end desaturases Ostreococcus sp. 'lucimarinus'
1.14.19.31 cytochrome b5 the enzyme exhibits a cytochrome b5 domain fused to its amino terminus and three histidine boxes that are typically found in front-end desaturases Euglena gracilis

General Information

EC Number General Information Comment Organism
1.14.19.31 evolution the enzyme belongs to the front-end desaturases Ostreococcus sp. 'lucimarinus'
1.14.19.31 additional information distribution of Egd4p desaturation products into different yeast lipid classes may indicate lipid-dependency Euglena gracilis
1.14.19.31 additional information distribution of Old4p desaturation products into different yeast lipid classes may indicate lipid-dependency Ostreococcus sp. 'lucimarinus'