EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.31 | gene Egd4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme | Euglena gracilis |
1.14.19.31 | gene Old4, DNA and amino acid sequence determination and analysis, phylogenetic tree, partly codon-optimized version of the cDNA, sequence comparison, recombinant expression in Saccharomyces cerevisiae strain INVSc1 in endoplasmic reticulum membrane,fatty acid analysis, overview. Expression of GFP-tagged enzyme, GFP-Old4p does not form aggregates in yeast cells and localizes to the endoplasmic reticulum | Ostreococcus sp. 'lucimarinus' |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.31 | endoplasmic reticulum membrane | - |
Ostreococcus sp. 'lucimarinus' | 5789 | - |
1.14.19.31 | endoplasmic reticulum membrane | - |
Euglena gracilis | 5789 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.31 | additional information | Ostreococcus sp. 'lucimarinus' | the enzyme catalyzes the production of docosahexaenoic acid | ? | - |
? | |
1.14.19.31 | additional information | Ostreococcus sp. 'lucimarinus' CCE9901 | the enzyme catalyzes the production of docosahexaenoic acid | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.31 | Euglena gracilis | Q6WNG7 | gene Egd4 | - |
1.14.19.31 | Euglena gracilis 1224-5/3 | Q6WNG7 | gene Egd4 | - |
1.14.19.31 | Ostreococcus sp. 'lucimarinus' | A4RQJ7 | gene Old4 | - |
1.14.19.31 | Ostreococcus sp. 'lucimarinus' CCE9901 | A4RQJ7 | gene Old4 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.31 | additional information | the enzyme catalyzes the production of docosahexaenoic acid | Ostreococcus sp. 'lucimarinus' | ? | - |
? | |
1.14.19.31 | additional information | substrate specificity, overview. The recombinant enzyme from Euglena gracilis expressed in Saccharomyces cerevisiae strain INVSc1 shows strongest activity of 15% toward 16:3(n-3) and desaturated 22:5(n-3) and 22:4(n-6) with efficiencies of 12 and 4%, respectively. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates | Euglena gracilis | ? | - |
? | |
1.14.19.31 | additional information | substrate specificity, the enzyme prefers very long chain polyunsaturated fatty acids, overview. The preferred substrate of the recombinant enzyme from Ostreococcus lucimarinus expressed in Saccharomyces cerevisiae strain INVSc1 with a desaturation activity of about 10% is 22:5(n-3) followed by 22:4(n-6) with an efficiency of about 4%. 16:3(n-3) is desaturated very poorly with an efficiency of about 1%. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates | Ostreococcus sp. 'lucimarinus' | ? | - |
? | |
1.14.19.31 | additional information | substrate specificity, overview. The recombinant enzyme from Euglena gracilis expressed in Saccharomyces cerevisiae strain INVSc1 shows strongest activity of 15% toward 16:3(n-3) and desaturated 22:5(n-3) and 22:4(n-6) with efficiencies of 12 and 4%, respectively. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates | Euglena gracilis 1224-5/3 | ? | - |
? | |
1.14.19.31 | additional information | the enzyme catalyzes the production of docosahexaenoic acid | Ostreococcus sp. 'lucimarinus' CCE9901 | ? | - |
? | |
1.14.19.31 | additional information | substrate specificity, the enzyme prefers very long chain polyunsaturated fatty acids, overview. The preferred substrate of the recombinant enzyme from Ostreococcus lucimarinus expressed in Saccharomyces cerevisiae strain INVSc1 with a desaturation activity of about 10% is 22:5(n-3) followed by 22:4(n-6) with an efficiency of about 4%. 16:3(n-3) is desaturated very poorly with an efficiency of about 1%. The enzyme has no clear preference for desaturation of (n-3)- or (n-6)-substrates | Ostreococcus sp. 'lucimarinus' CCE9901 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.31 | DELTA4-desaturase | - |
Ostreococcus sp. 'lucimarinus' |
1.14.19.31 | DELTA4-desaturase | - |
Euglena gracilis |
1.14.19.31 | DELTA4-fatty acid desaturase | - |
Ostreococcus sp. 'lucimarinus' |
1.14.19.31 | DELTA4-fatty acid desaturase | - |
Euglena gracilis |
1.14.19.31 | Egd4p | - |
Euglena gracilis |
1.14.19.31 | Old4p | - |
Ostreococcus sp. 'lucimarinus' |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.31 | cytochrome b5 | the enzyme exhibits a cytochrome b5 domain fused to its amino terminus and three histidine boxes that are typically found in front-end desaturases | Ostreococcus sp. 'lucimarinus' | |
1.14.19.31 | cytochrome b5 | the enzyme exhibits a cytochrome b5 domain fused to its amino terminus and three histidine boxes that are typically found in front-end desaturases | Euglena gracilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.31 | evolution | the enzyme belongs to the front-end desaturases | Ostreococcus sp. 'lucimarinus' |
1.14.19.31 | additional information | distribution of Egd4p desaturation products into different yeast lipid classes may indicate lipid-dependency | Euglena gracilis |
1.14.19.31 | additional information | distribution of Old4p desaturation products into different yeast lipid classes may indicate lipid-dependency | Ostreococcus sp. 'lucimarinus' |