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Literature summary extracted from

  • Tanaka, K.; Okabayashi, K.; Asashima, M.; Perrimon, N.; Kadowaki, T.
    The evolutionarily conserved porcupine family is involved in the processing of the Wnt family (2000), Eur. J. Biochem., 267, 4300-4311.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.250 gene MG61, DNA and amino acid sequence determination and analysis, sequence comparisons Homo sapiens
2.3.1.250 gene mom-1, DNA and amino acid sequence determination and analysis, sequence comparisons Caenorhabditis elegans
2.3.1.250 gene Porc, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in HEK-293 cells with or without co-expression of Wg Drosophila melanogaster
2.3.1.250 gene porc, expression of the enzyme tagged with three HA epitopes at amino acid 28. Capped porc, porc3HA28, DELTA34, DELTA66, and DELTA110 RNA are prepared by in vitro transcription and RNA is injected into Drosophila embryos Drosophila melanogaster
2.3.1.250 gene Xporc, DNA and amino acid sequence determination and analysis, sequence comparisons Xenopus laevis
2.3.1.250 single gene Mporc, variants by alternative splicing, DNA and amino acid sequence determination and analysis, sequence comparisons, co-expression in COS-1 cells of HA-tagged Mporc and either Myc-tagged Wnt or partial laminin beta chain, recombinant expression in HEK-293 cells with or without co-expression of Drosophila melanogaster Wnt variant Wg Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.250 additional information generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview Drosophila melanogaster
2.3.1.250 additional information rescue of Drosophila melanogaster porc embryos by Mporc RNA injection Mus musculus
2.3.1.250 additional information rescue of Drosophila porc embryos by Mus musculus Mporc RNA injection Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.250 endoplasmic reticulum membrane a multispanning transmembrane protein Drosophila melanogaster 5789
-
2.3.1.250 endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Homo sapiens 5789
-
2.3.1.250 endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Mus musculus 5789
-
2.3.1.250 endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Xenopus laevis 5789
-
2.3.1.250 endoplasmic reticulum membrane the enzyme has multiple transmembrane domains Caenorhabditis elegans 5789
-
2.3.1.250 endoplasmic reticulum membrane the enzyme has multiple transmembrane domains, Drosophila Porc is localized at the endoplasmic reticulum when expressed in Drosophila cells as an epitope-tagged protein Drosophila melanogaster 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine Drosophila melanogaster
-
 CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine Drosophila melanogaster
-
 CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine Drosophila melanogaster the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Homo sapiens
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Mus musculus
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Drosophila melanogaster
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Xenopus laevis
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine Caenorhabditis elegans
-
 CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 additional information Drosophila melanogaster enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.250 Caenorhabditis elegans Q22329 gene mom-1; gene mom-1
-
2.3.1.250 Drosophila melanogaster
-
 gene porcupine, i.e. porc
-
2.3.1.250 Drosophila melanogaster Q9VWV9 gene Porc or CG6205
-
2.3.1.250 Homo sapiens Q9H237 gene porc
-
2.3.1.250 Mus musculus Q9JJJ7 single gene and alternative splicing resulting in several variants
-
2.3.1.250 Xenopus laevis Q9I935 gene Xporc
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.250 cell culture
-
 Drosophila melanogaster
-
2.3.1.250 embryo
-
 Drosophila melanogaster
-
2.3.1.250 embryo
-
 Mus musculus
-
2.3.1.250 fibroblast
-
 Mus musculus
-
2.3.1.250 HEK-293 cell
-
 Homo sapiens
-
2.3.1.250 additional information Mporc mRNA is differentially expressed during embryogenesis and in various adult tissues, demonstrating that the alternative splicing is regulated to synthesize the specific types of Mporc, pattern of expression of Mporc at fetal and adult stages of development, overview Mus musculus
-
2.3.1.250 oocyte
-
 Xenopus laevis
-
2.3.1.250 S2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine
-
 Drosophila melanogaster CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine Wnt substrate from Drosophila melanogaster Mus musculus CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
-
 Drosophila melanogaster CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
-
 Drosophila melanogaster CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg Drosophila melanogaster CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg. The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg Drosophila melanogaster CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Homo sapiens CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Mus musculus CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Drosophila melanogaster CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Xenopus laevis CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 (9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
-
 Caenorhabditis elegans CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
 ?
2.3.1.250 additional information enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity Drosophila melanogaster ?
-
 ?
2.3.1.250 additional information recombinant c-Myc-tagged substrate Wg mutants T51A, S105A, S110A, and T416A Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.250 CG6205
-
 Drosophila melanogaster
2.3.1.250 MG61
-
 Homo sapiens
2.3.1.250 Mom-1
-
 Caenorhabditis elegans
2.3.1.250 Mporc
-
 Mus musculus
2.3.1.250 PORC
-
 Drosophila melanogaster
2.3.1.250 PORC
-
 Homo sapiens
2.3.1.250 PORCN
-
 Homo sapiens
2.3.1.250 PORCN
-
 Caenorhabditis elegans
2.3.1.250 PPN
-
 Homo sapiens
2.3.1.250 Xporc
-
 Xenopus laevis

General Information

EC Number General Information Comment Organism
2.3.1.250 evolution Drosophila Porc has an extra hydrophilic N-terminal sequence, which is not found in other Porc enzymes Drosophila melanogaster
2.3.1.250 additional information all Mporc types can substitute for Drosophila Porc, as they are able to rescue the phenotypes of Drosophila porc embryos, the enzyme modifies the processing of Drosophila Wg protein expressed in mammalian cells Mus musculus
2.3.1.250 additional information all Mus musculus Mporc types can substitute for Drosophila Porc, as they are able to rescue the phenotypes of Drosophila porc embryos Drosophila melanogaster
2.3.1.250 additional information model for the N-glycosylation of Wnt family, overview Drosophila melanogaster
2.3.1.250 physiological function ectopic expression of porc stimulates the N-glycosylation of both endogenously and exogenously expressed protein Wingless (Wg). The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg, which is highly conserved in the Wnt family and stimulates the N-glycosylation at surrounding sites. The enzyme is also necessary for the processing of Drosophila Wnt-3/5 in both embryos and cultured cells. The enzyme binds the N-terminal specific domain of the Wnt family via its C-terminnus and stimulates its posttranslational N-glycosylation by anchoring them at the endoplasmic reticulum membrane possibly through acylation, overview Drosophila melanogaster
2.3.1.250 physiological function Mporc binds Wnt proteins and modifies their processing, the enzyme may function as a chaperone-like molecule for Wnt, overview Mus musculus
2.3.1.250 physiological function the enzyme is necessary for processing Wingless (Wg), a Drosophila Wnt (Wnt) family member. The enzyme modifies the processing of substrate Wg expressed in mammalian cells Drosophila melanogaster