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Literature summary extracted from

  • Schweiger, G.; Dutscho R.; Buckel, W.
    Purification of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. An iron-sulfur protein (1987), Eur. J. Biochem., 169, 441-448.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.167 Activator protein the enzyme must be activated by an activator protein. Once activated, it can catalyse many turnovers Acidaminococcus fermentans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.167 [4Fe-4S]2+ cluster
-
 Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.167 42000
-
 1 * 55000, 1 * 42000, SDS-PAGE Acidaminococcus fermentans
4.2.1.167 42000
-
 2 * 55000 (alpha), + 2 * 42000 (beta), SDS-PAGE Acidaminococcus fermentans
4.2.1.167 55000
-
 1 * 55000, 1 * 42000, SDS-PAGE Acidaminococcus fermentans
4.2.1.167 55000
-
 2 * 55000 (alpha), + 2 * 42000 (beta), SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.167 (R)-2-hydroxyglutaryl-CoA Acidaminococcus fermentans the enzyme is involved in the fermentation of L-glutamate (E)-glutaconyl-CoA + H2O
-
 ?
4.2.1.167 (R)-2-hydroxyglutaryl-CoA Acidaminococcus fermentans DSM 20731 the enzyme is involved in the fermentation of L-glutamate (E)-glutaconyl-CoA + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.167 Acidaminococcus fermentans P11569 and P11570
-
-
4.2.1.167 Acidaminococcus fermentans P11569 and P11570 P11569: alpha-subunit, P11570: beta-subunit
-
4.2.1.167 Acidaminococcus fermentans DSM 20731 P11569 and P11570
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.167
-
 Acidaminococcus fermentans
4.2.1.167 chromatography on blue-Sepharose Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.167 0.0558
-
 pH 7.0, 37°C Acidaminococcus fermentans

Storage Stability

EC Number Storage Stability Organism
4.2.1.167 4°C, stable for several months Acidaminococcus fermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
 ?
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
 r
4.2.1.167 (R)-2-hydroxyglutaryl-CoA the enzyme is involved in the fermentation of L-glutamate Acidaminococcus fermentans (E)-glutaconyl-CoA + H2O
-
 ?
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
 ?
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
 r
4.2.1.167 (R)-2-hydroxyglutaryl-CoA the enzyme is involved in the fermentation of L-glutamate Acidaminococcus fermentans DSM 20731 (E)-glutaconyl-CoA + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.167 heterodimer 1 * 55000, 1 * 42000, SDS-PAGE Acidaminococcus fermentans
4.2.1.167 tetramer 2 * 55000 (alpha), + 2 * 42000 (beta), SDS-PAGE Acidaminococcus fermentans

Synonyms

EC Number Synonyms Comment Organism
4.2.1.167 hgdAB
-
 Acidaminococcus fermentans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.167 37
-
 assay at Acidaminococcus fermentans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.167 7
-
 assay at Acidaminococcus fermentans

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.167 4Fe-4S-center the enzyme contains [4F-4S] clusters Acidaminococcus fermentans

General Information

EC Number General Information Comment Organism
4.2.1.167 metabolism the enzyme is involved in the fermentation of L-glutamate Acidaminococcus fermentans