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Literature summary extracted from

  • Wang, L.; Zhou, Y.J.; Ji, D.; Lin, X.; Liu, Y.; Zhang, Y.; Liu, W.; Zhao, Z.K.
    Identification of UshA as a major enzyme for NAD degradation in Escherichia coli (2014), Enzyme Microb. Technol., 58-59, 75-79.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.22 0.0053
-
 NAD+ pH 7.5, 30°C Escherichia coli
3.6.1.22 0.007
-
 NADH pH 7.5, 30°C Escherichia coli
3.6.1.22 0.0111
-
 NMN pH 7.5, 30°C Escherichia coli
3.6.1.45 0.0012
-
 AMP pH 7.5, 30°C Escherichia coli
3.6.1.45 0.0053
-
 NAD+ pH 7.5, 30°C Escherichia coli
3.6.1.45 0.007
-
 NADH pH 7.5, 30°C Escherichia coli
3.6.1.45 0.0111
-
 NMN pH 7.5, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.22 Escherichia coli B1XCS0
-
-
3.6.1.22 Escherichia coli DH10B B1XCS0
-
-
3.6.1.45 Escherichia coli B1XCS0
-
-
3.6.1.45 Escherichia coli DH10B B1XCS0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.22 NAD+ + H2O
-
 Escherichia coli AMP + NMN
-
 ?
3.6.1.22 NADH + H2O
-
 Escherichia coli NMNH + AMP
-
 ?
3.6.1.22 NADH + H2O
-
 Escherichia coli DH10B NMNH + AMP
-
 ?
3.6.1.22 NMN + H2O
-
 Escherichia coli nicotinamide riboside + phosphate
-
 ?
3.6.1.45 AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.6.1.45 AMP + H2O
-
 Escherichia coli DH10B adenosine + phosphate
-
 ?
3.6.1.45 NAD+ + H2O
-
 Escherichia coli AMP + NMN
-
 ?
3.6.1.45 NAD+ + H2O
-
 Escherichia coli DH10B AMP + NMN
-
 ?
3.6.1.45 NADH + H2O
-
 Escherichia coli NMNH + AMP
-
 ?
3.6.1.45 NADH + H2O
-
 Escherichia coli DH10B NMNH + AMP
-
 ?
3.6.1.45 NMN + H2O
-
 Escherichia coli nicotinamide riboside + phosphate
-
 ?
3.6.1.45 NMN + H2O
-
 Escherichia coli DH10B nicotinamide riboside + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.22 UshA
-
 Escherichia coli
3.6.1.45 UshA
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.22 10
-
 NADH pH 7.5, 30°C Escherichia coli
3.6.1.22 19.3
-
 NAD+ pH 7.5, 30°C Escherichia coli
3.6.1.22 1492
-
 NMN pH 7.5, 30°C Escherichia coli
3.6.1.45 10
-
 NADH pH 7.5, 30°C Escherichia coli
3.6.1.45 19.3
-
 NAD+ pH 7.5, 30°C Escherichia coli
3.6.1.45 405
-
 AMP pH 7.5, 30°C Escherichia coli
3.6.1.45 1492
-
 NMN pH 7.5, 30°C Escherichia coli

General Information

EC Number General Information Comment Organism
3.6.1.22 physiological function deletion of the UshA gene leads to faster cell growth and improves extracellular NAD stability by 3fold under conditions similar to whole-cell biocatalysis Escherichia coli
3.6.1.45 physiological function deletion of the UshA gene leads to faster cell growth and improves extracellular NAD stability by 3fold under conditions similar to whole-cell biocatalysis Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.22 1400
-
 NADH pH 7.5, 30°C Escherichia coli
3.6.1.22 3700
-
 NAD+ pH 7.5, 30°C Escherichia coli
3.6.1.22 135000
-
 NMN pH 7.5, 30°C Escherichia coli
3.6.1.45 1400
-
 NADH pH 7.5, 30°C Escherichia coli
3.6.1.45 3700
-
 NAD+ pH 7.5, 30°C Escherichia coli
3.6.1.45 135000
-
 NMN pH 7.5, 30°C Escherichia coli
3.6.1.45 334000
-
 AMP pH 7.5, 30°C Escherichia coli