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Literature summary extracted from

  • Petronikolou, N.; Nair, S.K.
    Biochemical studies of mycobacterial fatty acid methyltransferase: A catalyst for the enzymatic production of biodiesel (2015), Chem. Biol., 22, 1480-1490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.15
-
 Mycobacterium marinum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.15 the cocrystal structure of with S-adenosyl-L-homocysteine and octanoate shows that the substrate-binding site is largely localized within the alpha-helical domain that caps the Rossmann-like fold domain. Aliphatic and aromatic residues encircle the acyl chain of the fatty acid substrate, including Trp155, Met214, Phe222, Tyr224, Val256, Phe311, and Leu316. In the cocrystal structure with 3-hydroxydecanoate, only the S enantiomer is bound in the active site Mycobacterium marinum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.15 Q154A 17fold increaseof the KM with octanoate as a substrate, and 5fold increase of the KM for 3-hydroxydecanoate Mycobacterium marinum
2.1.1.15 Q31A complete loss of activtiy Mycobacterium marinum
2.1.1.15 W155F near 1000fold decrease in catalytic efficiency Mycobacterium marinum
2.1.1.15 Y24F 30fold decrease in catalytic efficiency Mycobacterium marinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.15 0.0082
-
 Decanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.0255
-
 S-adenosyl-L-methionine wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.201
-
 3-hydroxydecanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.308
-
 octanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 1.03
-
 3-hydroxydecanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 2.039
-
 3-hydroxyoctanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 5.24
-
 octanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.15 Mycobacterium marinum B2HHT4
-
-
2.1.1.15 Mycobacterium marinum ATCC BAA-535 B2HHT4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.15 3-hydroxydecanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 3-hydroxydecanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum ATCC BAA-535 methyl 3-hydroxydecanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 3-hydroxyoctanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 3-hydroxyoctanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum ATCC BAA-535 methyl 3-hydroxyoctanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 decanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl decanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 decanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum ATCC BAA-535 methyl decanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 octanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum methyl octanoate + S-adenosyl-L-homocysteine
-
 ?
2.1.1.15 octanoic acid + S-adenosyl-L-methionine
-
 Mycobacterium marinum ATCC BAA-535 methyl octanoate + S-adenosyl-L-homocysteine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.15 FAMT
-
 Mycobacterium marinum
2.1.1.15 MMAR_3356
-
 Mycobacterium marinum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.15 0.042
-
 3-hydroxydecanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.147
-
 octanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.244
-
 Decanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.461
-
 octanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.488
-
 3-hydroxyoctanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.598
-
 S-adenosyl-L-methionine wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.599
-
 3-hydroxydecanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.15 0.0014
-
 octanoic acid mutant W155F, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.0031
-
 3-hydroxydecanoic acid mutant W155F, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.028
-
 octanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.041
-
 3-hydroxydecanoic acid mutant Q31A, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.239
-
 3-hydroxyoctanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 0.824
-
 S-adenosyl-L-methionine mutant Y24F, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 1.46
-
 octanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 2.98
-
 3-hydroxydecanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 23.45
-
 S-adenosyl-L-methionine wild-type, pH 7.8, 37°C Mycobacterium marinum
2.1.1.15 29.76
-
 Decanoic acid wild-type, pH 7.8, 37°C Mycobacterium marinum