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Effects of nicotinamide N-methyltransferase on PANC-1 cells proliferation, metastatic potential and survival under metabolic stress

Yu, T.; Wang, Y.T.; Chen, P.; Li, Y.H.; Chen, Y.X.; Zeng, H.; Yu, A.M.; Huang, M.; Bi, H.C.; Cell. Physiol. Biochem. 35, 710-721 (2015)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.1.1.1
diagnostics
the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target
Homo sapiens
2.1.1.1
medicine
the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.1
recombinant enzyme overexpression in PANC-1 cells, quantitative real-time PCR enzyme expression analysis
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.1
additional information
transfection of pancreatic cancer cell line PANC-1 with NNMT expression plasmid or small interfering RNA of NNMT to overexpress or knockdown intracellular NNMT expression, respectively. Enzyme silencing markedly reduces cell proliferation, whereas enzyme overexpression promotes cell growth moderately. Enzyme knockdown by specific siRNA
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
PANC-1 cell
-
Homo sapiens
-
2.1.1.1
pancreatic cancer cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733636
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.1.1.1
diagnostics
the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target
Homo sapiens
2.1.1.1
medicine
the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
recombinant enzyme overexpression in PANC-1 cells, quantitative real-time PCR enzyme expression analysis
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.1
additional information
transfection of pancreatic cancer cell line PANC-1 with NNMT expression plasmid or small interfering RNA of NNMT to overexpress or knockdown intracellular NNMT expression, respectively. Enzyme silencing markedly reduces cell proliferation, whereas enzyme overexpression promotes cell growth moderately. Enzyme knockdown by specific siRNA
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
PANC-1 cell
-
Homo sapiens
-
2.1.1.1
pancreatic cancer cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733636
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.1.1.1
malfunction
enzyme NNMT plays an important role in PANC-1 cell proliferation, metastatic potential and survival under metabolic stress. Enzyme silencing markedly reduces cell proliferation, whereas enzyme overexpression promotes cell growth moderately. Knockdown of NNMT also significantly suppresses the migration and invasion capacities of pancreatic cancer PANC-1 cells. Enzyme NNMT upregulation enhances cell migration and invasion capacities. In addition, NNMT knockdown cells are much less resistant to glucose deprivation and rapamycin as well as glycolytic inhibitor 2-deoxyglucose whereas NNMT-overexpressing cells show opposite effects although the effects are not so striking
Homo sapiens
2.1.1.1
physiological function
effect of enzyme NNMT expression on cell survival under metabolic stress, the enzyme promotes cell survival and growth and increases cell migra­tion and invasion ca­pacities , NNMT-expressing cells demonstrate enhanced resistance to rapamycin, phenotype, overview
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.1
malfunction
enzyme NNMT plays an important role in PANC-1 cell proliferation, metastatic potential and survival under metabolic stress. Enzyme silencing markedly reduces cell proliferation, whereas enzyme overexpression promotes cell growth moderately. Knockdown of NNMT also significantly suppresses the migration and invasion capacities of pancreatic cancer PANC-1 cells. Enzyme NNMT upregulation enhances cell migration and invasion capacities. In addition, NNMT knockdown cells are much less resistant to glucose deprivation and rapamycin as well as glycolytic inhibitor 2-deoxyglucose whereas NNMT-overexpressing cells show opposite effects although the effects are not so striking
Homo sapiens
2.1.1.1
physiological function
effect of enzyme NNMT expression on cell survival under metabolic stress, the enzyme promotes cell survival and growth and increases cell migra­tion and invasion ca­pacities , NNMT-expressing cells demonstrate enhanced resistance to rapamycin, phenotype, overview
Homo sapiens