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Literature summary extracted from

  • Yu, T.; Wang, Y.T.; Chen, P.; Li, Y.H.; Chen, Y.X.; Zeng, H.; Yu, A.M.; Huang, M.; Bi, H.C.
    Effects of nicotinamide N-methyltransferase on PANC-1 cells proliferation, metastatic potential and survival under metabolic stress (2015), Cell. Physiol. Biochem., 35, 710-721.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.1 diagnostics the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target Homo sapiens
2.1.1.1 medicine the enzyme may serve as a potential biomarker for tumor diagnosis and a therapeutic target Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.1 recombinant enzyme overexpression in PANC-1 cells, quantitative real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.1 additional information transfection of pancreatic cancer cell line PANC-1 with NNMT expression plasmid or small interfering RNA of NNMT to overexpress or knockdown intracellular NNMT expression, respectively. Enzyme silencing markedly reduces cell proliferation, whereas enzyme overexpression promotes cell growth moderately. Enzyme knockdown by specific siRNA Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.1 S-adenosyl-L-methionine + nicotinamide Homo sapiens
-
 S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.1 PANC-1 cell
-
 Homo sapiens
-
2.1.1.1 pancreatic cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.1 NNMT
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.1 S-adenosyl-L-methionine
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.1.1 malfunction enzyme NNMT plays an important role in PANC-1 cell proliferation, metastatic potential and survival under metabolic stress. Enzyme silencing markedly reduces cell proliferation, whereas enzyme overexpression promotes cell growth moderately. Knockdown of NNMT also significantly suppresses the migration and invasion capacities of pancreatic cancer PANC-1 cells. Enzyme NNMT upregulation enhances cell migration and invasion capacities. In addition, NNMT knockdown cells are much less resistant to glucose deprivation and rapamycin as well as glycolytic inhibitor 2-deoxyglucose whereas NNMT-overexpressing cells show opposite effects although the effects are not so striking Homo sapiens
2.1.1.1 physiological function effect of enzyme NNMT expression on cell survival under metabolic stress, the enzyme promotes cell survival and growth and increases cell migra­tion and invasion ca­pacities , NNMT-expressing cells demonstrate enhanced resistance to rapamycin, phenotype, overview Homo sapiens