BRENDA - Enzyme Database

Nicotinamide N-methyltransferase in non-small cell lung cancer: promising results for targeted anti-cancer therapy

Sartini, D.; Morganti, S.; Guidi, E.; Rubini, C.; Zizzi, A.; Giuliante, R.; Pozzi, V.; Emanuelli, M.; Cell Biochem. Biophys. 67, 865-873 (2013)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.1.1.1
drug development
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
2.1.1.1
pharmacology
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.1.1.1
quantitative real-time PCR enzyme expression analysis in non-smalll cell lung cancer samples
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
A-549 cell
-
Homo sapiens
-
2.1.1.1
additional information
quantitative real-time PCR enzyme expression analysis
Homo sapiens
-
2.1.1.1
non-small cell lung cancer cell
the cells show enzyme overexpression compared to both tumor-adjacent and surrounding tissue
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
quantitative enzyme activity analysis in 25 non-smalll cell lung cancer samples
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733618
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.1.1.1
NNMT
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
8.6
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.1.1.1
drug development
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
2.1.1.1
pharmacology
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
quantitative real-time PCR enzyme expression analysis in non-smalll cell lung cancer samples
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
A-549 cell
-
Homo sapiens
-
2.1.1.1
additional information
quantitative real-time PCR enzyme expression analysis
Homo sapiens
-
2.1.1.1
non-small cell lung cancer cell
the cells show enzyme overexpression compared to both tumor-adjacent and surrounding tissue
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
quantitative enzyme activity analysis in 25 non-smalll cell lung cancer samples
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733618
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
8.6
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.1.1.1
malfunction
shRNA-mediated gene silencing of NNMT leads to a significntl inhibition of cell proliferation and colony formation ability, downregulation of the enzyme significantly reduces the tumorigenicity of A-549 cells
Homo sapiens
2.1.1.1
physiological function
role of nicotinamide N-methyltransferase in non-small cell lung cancer
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.1
malfunction
shRNA-mediated gene silencing of NNMT leads to a significntl inhibition of cell proliferation and colony formation ability, downregulation of the enzyme significantly reduces the tumorigenicity of A-549 cells
Homo sapiens
2.1.1.1
physiological function
role of nicotinamide N-methyltransferase in non-small cell lung cancer
Homo sapiens