BRENDA - Enzyme Database show

Nicotinamide N-methyltransferase in non-small cell lung cancer: promising results for targeted anti-cancer therapy

Sartini, D.; Morganti, S.; Guidi, E.; Rubini, C.; Zizzi, A.; Giuliante, R.; Pozzi, V.; Emanuelli, M.; Cell Biochem. Biophys. 67, 865-873 (2013)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.1.1.1
drug development
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
2.1.1.1
pharmacology
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.1
quantitative real-time PCR enzyme expression analysis in non-smalll cell lung cancer samples
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
A-549 cell
-
Homo sapiens
-
2.1.1.1
additional information
quantitative real-time PCR enzyme expression analysis
Homo sapiens
-
2.1.1.1
non-small cell lung cancer cell
the cells show enzyme overexpression compared to both tumor-adjacent and surrounding tissue
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
quantitative enzyme activity analysis in 25 non-smalll cell lung cancer samples
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733618
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
8.6
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.1.1.1
drug development
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
2.1.1.1
pharmacology
the enzyme might be a good molecular target for lung cancer therapy
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.1
quantitative real-time PCR enzyme expression analysis in non-smalll cell lung cancer samples
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.1.1.1
S-adenosyl-L-methionine
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
Homo sapiens
-
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.1.1
A-549 cell
-
Homo sapiens
-
2.1.1.1
additional information
quantitative real-time PCR enzyme expression analysis
Homo sapiens
-
2.1.1.1
non-small cell lung cancer cell
the cells show enzyme overexpression compared to both tumor-adjacent and surrounding tissue
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.1.1.1
additional information
-
quantitative enzyme activity analysis in 25 non-smalll cell lung cancer samples
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.1
S-adenosyl-L-methionine + nicotinamide
-
733618
Homo sapiens
S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.1.1.1
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.1.1
8.6
-
assay at
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
2.1.1.1
malfunction
shRNA-mediated gene silencing of NNMT leads to a significntl inhibition of cell proliferation and colony formation ability, downregulation of the enzyme significantly reduces the tumorigenicity of A-549 cells
Homo sapiens
2.1.1.1
physiological function
role of nicotinamide N-methyltransferase in non-small cell lung cancer
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.1.1.1
malfunction
shRNA-mediated gene silencing of NNMT leads to a significntl inhibition of cell proliferation and colony formation ability, downregulation of the enzyme significantly reduces the tumorigenicity of A-549 cells
Homo sapiens
2.1.1.1
physiological function
role of nicotinamide N-methyltransferase in non-small cell lung cancer
Homo sapiens