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Literature summary extracted from
- Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives (2015), Biotechnol. Lett., 37, 1655-1661.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.5.5 | expressed in Escherichia coli BL21(DE3) cells | Luminiphilus syltensis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.5 | 0.38 | - |
2-chloromandelonitrile | at pH 7.0 and 30°C | Luminiphilus syltensis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 43000 | - |
x * 43000, SDS-PAGE | Luminiphilus syltensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.5 | Luminiphilus syltensis | - |
- |
- |
| 3.5.5.5 | Luminiphilus syltensis NOR5-1B | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.5 | Ni-NTA column chromatography | Luminiphilus syltensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.5 | (2-bromophenyl)(hydroxy)acetonitrile + H2O | 74% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(2-bromophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (2-fluorophenyl)(hydroxy)acetonitrile + H2O | 439% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(2-fluorophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (3-bromophenyl)(hydroxy)acetonitrile + H2O | 706% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(3-bromophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (3-chlorophenyl)(hydroxy)acetonitrile + H2O | 810% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(3-chlorophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (3-fluorophenyl)(hydroxy)acetonitrile + H2O | 454% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(3-fluorophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (4-bromophenyl)(hydroxy)acetonitrile + H2O | 680% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(4-bromophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (4-chlorophenyl)(hydroxy)acetonitrile + H2O | 1083% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(4-chlorophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | (4-fluorophenyl)(hydroxy)acetonitrile + H2O | 891% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-(4-fluorophenyl)(hydroxy)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | 1,2-phenylenediacetonitrile + H2O | 1.87% activity compared to phenylacetonitrile | Luminiphilus syltensis | 1,2-phenylenediacetic acid + NH3 | - |
? | |
| 3.5.5.5 | 2-chloromandelonitrile + H2O | 33.75% activity compared to phenylacetonitrile | Luminiphilus syltensis | (R)-2-chloromandelic acid + NH3 | enantioselectivity ee 98.2% | ? | |
| 3.5.5.5 | 2-cyanopyridine + 2 H2O | 101% activity compared to phenylacetonitrile | Luminiphilus syltensis | pyridine 2-carboxylic acid + NH3 | - |
? | |
| 3.5.5.5 | 2-methylglutaronitrile + 4 H2O | 0.12% activity compared to phenylacetonitrile | Luminiphilus syltensis | 2-methylglutaric acid + 2 NH3 | - |
? | |
| 3.5.5.5 | 2-phenylbutyronitrile + H2O | 0.2% activity compared to phenylacetonitrile | Luminiphilus syltensis | 2-phenylbutyric acid + NH3 | - |
? | |
| 3.5.5.5 | 3-cyanopyridine + 2 H2O | 43.2% activity compared to phenylacetonitrile | Luminiphilus syltensis | pyridine 3-carboxylic acid + NH3 | - |
? | |
| 3.5.5.5 | 3-hydroxyglutaronitrile + 4 H2O | 0.12% activity compared to phenylacetonitrile | Luminiphilus syltensis | 3-hydroxyglutaric acid + 2 NH3 | - |
? | |
| 3.5.5.5 | 3-hydroxyphenylpropionitrile + H2O | 0.12% activity compared to phenylacetonitrile | Luminiphilus syltensis | 3-hydroxyphenylpropionic acid + NH3 | - |
? | |
| 3.5.5.5 | 3-hydroxypropionitrile + H2O | 0.07% activity compared to phenylacetonitrile | Luminiphilus syltensis | 3-hydroxypropionic acid + NH3 | - |
? | |
| 3.5.5.5 | 4-chlorobutyronitrile + H2O | 0.06% activity compared to phenylacetonitrile | Luminiphilus syltensis | 4-chlorobutyric acid + NH3 | - |
? | |
| 3.5.5.5 | 4-cyanopyridine + 2 H2O | 142% activity compared to phenylacetonitrile | Luminiphilus syltensis | pyridine 4-carboxylic acid + NH3 | - |
? | |
| 3.5.5.5 | acrylonitrile + H2O | 0.05% activity compared to phenylacetonitrile | Luminiphilus syltensis | acrylic acid + NH3 | - |
? | |
| 3.5.5.5 | acrylonitrile + H2O | 0.05% activity compared to phenylacetonitrile | Luminiphilus syltensis NOR5-1B | acrylic acid + NH3 | - |
? | |
| 3.5.5.5 | alpha-methylphenylacetonitrile + H2O | 0.14% activity compared to phenylacetonitrile | Luminiphilus syltensis | alpha-methylphenylacetic acid + NH3 | - |
? | |
| 3.5.5.5 | benzonitrile + 2 H2O | 21% activity compared to phenylacetonitrile | Luminiphilus syltensis | benzoic acid + NH3 | - |
? | |
| 3.5.5.5 | cinnamonitrile + H2O | 0.07% activity compared to phenylacetonitrile | Luminiphilus syltensis | cinnamic acid + NH3 | - |
? | |
| 3.5.5.5 | dodecanenitrile + H2O | 0.04% activity compared to phenylacetonitrile | Luminiphilus syltensis | dodecanoic acid + NH3 | - |
? | |
| 3.5.5.5 | fumaronitrile + H2O | 0.72% activity compared to phenylacetonitrile | Luminiphilus syltensis | fumaric acid + NH3 | - |
? | |
| 3.5.5.5 | fumaronitrile + H2O | 0.72% activity compared to phenylacetonitrile | Luminiphilus syltensis NOR5-1B | fumaric acid + NH3 | - |
? | |
| 3.5.5.5 | glycolonitrile + H2O | 0.08% activity compared to phenylacetonitrile | Luminiphilus syltensis | glycolic acid + NH3 | - |
? | |
| 3.5.5.5 | hydroxy(2-methoxyphenyl)acetonitrile + H2O | 178% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-hydroxy(2-methoxyphenyl)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | hydroxy(2-methylphenyl)acetonitrile + H2O | 194% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-hydroxy(2-methylphenyl)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | hydroxy(phenyl)acetonitrile + H2O | 404% activity compared to phenylacetonitrile | Luminiphilus syltensis | (2R)-hydroxy(phenyl)acetic acid + NH3 | - |
? | |
| 3.5.5.5 | iminodiacetonitrile + 2 H2O | 0.05% activity compared to phenylacetonitrile | Luminiphilus syltensis | iminodiacetic acid + 2 NH3 | - |
? | |
| 3.5.5.5 | iminodiacetonitrile + 2 H2O | 0.05% activity compared to phenylacetonitrile | Luminiphilus syltensis NOR5-1B | iminodiacetic acid + 2 NH3 | - |
? | |
| 3.5.5.5 | indole-3-acetonitrile + H2O | 35% activity compared to phenylacetonitrile | Luminiphilus syltensis | indole-3-acetic acid + NH3 | - |
? | |
| 3.5.5.5 | malononitrile + H2O | 3.7% activity compared to phenylacetonitrile | Luminiphilus syltensis | malonic acid + NH3 | - |
? | |
| 3.5.5.5 | malononitrile + H2O | 3.7% activity compared to phenylacetonitrile | Luminiphilus syltensis NOR5-1B | malonic acid + NH3 | - |
? | |
| 3.5.5.5 | mandelonitrile + 2 H2O | best substrate, 164% activity compared to phenylacetonitrile | Luminiphilus syltensis | mandelic acid + NH3 | - |
? | |
| 3.5.5.5 | mandelonitrile + 2 H2O | best substrate, 164% activity compared to phenylacetonitrile | Luminiphilus syltensis NOR5-1B | mandelic acid + NH3 | - |
? | |
| 3.5.5.5 | octanedinitrile + H2O | 0.14% activity compared to phenylacetonitrile | Luminiphilus syltensis | ? + NH3 | - |
? | |
| 3.5.5.5 | succinonitrile + H2O | 0.12% activity compared to phenylacetonitrile | Luminiphilus syltensis | succinic acid + NH3 | - |
? | |
| 3.5.5.5 | valeronitrile + H2O | 0.08% activity compared to phenylacetonitrile | Luminiphilus syltensis | valeric acid + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.5.5 | ? | x * 43000, SDS-PAGE | Luminiphilus syltensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.5.5 | arylacetonitrile-specific nitrilase | - |
Luminiphilus syltensis |
| 3.5.5.5 | GPnor51 | - |
Luminiphilus syltensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 40 | - |
- |
Luminiphilus syltensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 30 | 45 | more than half maximal activity at 30 and 45°C | Luminiphilus syltensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 50 | - |
the enzyme retains 50% of its initial activity after incubation at 50°C for 30 min | Luminiphilus syltensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.5 | 7 | - |
- |
Luminiphilus syltensis |
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