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Literature summary extracted from

  • Zhang, Q.; Xu, H.; Zhao, J.; Zeng, R.
    Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library (2014), Biotechnol. Lett., 36, 617-625.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 gene PGA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, cloning and recombinant expression of His-tagged enzyme in Escherichia coli strain M15 uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.11 Ca2+ inhibits 15.1% at 1 mM uncultured bacterium
3.5.1.11 Cu2+ inhibits 61.7% at 1 mM uncultured bacterium
3.5.1.11 dimethylformamide inhibits 10.5% at 10% and 33.2% at 40% uncultured bacterium
3.5.1.11 EDTA inhibits 80.4% at 10 mM uncultured bacterium
3.5.1.11 Fe3+ inhibits 96.0% at 1 mM uncultured bacterium
3.5.1.11 Mn2+ inhibits 66.2% at 1 mM uncultured bacterium
3.5.1.11 SDS inhibits 56.0% at 2% uncultured bacterium
3.5.1.11 Sr2+ inhibits 95.4% at 1 mM uncultured bacterium
3.5.1.11 Urea inhibits 95.7% at 8 M, no inhibition at 4 M uncultured bacterium
3.5.1.11 Zn2+ inhibits 12.5% at 1 mM uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.11 0.0046
-
 penicillin G pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0083
-
 6-nitro-3-(phenylacetamido)-benzoic acid pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0156
-
 cephalothin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0164
-
 cephaloridine pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0255
-
 cephalexin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0393
-
 Penicillin V pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0519
-
 carbenicillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.0944
-
 ampicillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 0.105
-
 amoxycillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.11 K+ activates 87.8% at 1 mM uncultured bacterium
3.5.1.11 Mg2+ activates 46.0% at 1 mM uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 26000
-
 1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
3.5.1.11 63000
-
 1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium
3.5.1.11 88000
-
 recombinant His-tagged enzyme, gel filtration uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin G + H2O uncultured bacterium
-
 phenylacetate + 6-aminopenicillanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 uncultured bacterium D6R1Q1 PGA gene from a metagenomic library of an Antarctic sample
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11 recombinant His-tagged enzyme from Escherichia coli strain M15 by affinity chromatography and gel filtration uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
 uncultured bacterium 3-amino-6-nitrobenzoic acid + phenylacetate
-
 ?
3.5.1.11 amoxycillin + H2O
-
 uncultured bacterium D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid
-
 ?
3.5.1.11 ampicillin + H2O
-
 uncultured bacterium 6-aminopenicillanic acid + (R)-2-phenylglycine
-
 ?
3.5.1.11 carbenicillin + H2O
-
 uncultured bacterium phenylpropanedioate + 6-aminopenicillate
-
 ?
3.5.1.11 cephalexin + H2O
-
 uncultured bacterium 7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine
-
 ?
3.5.1.11 cephaloridine + H2O
-
 uncultured bacterium thiophen-2-ylacetic acid + (6R,7R)-7-amino-8-oxo-3-(pyridinium-1-yl-methyl)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.5.1.11 cephalothin + H2O
-
 uncultured bacterium thiophen-2-ylacetic acid + (6R,7R)-3-acetoxymethyl-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.5.1.11 penicillin G + H2O
-
 uncultured bacterium phenylacetate + 6-aminopenicillanate
-
 ?
3.5.1.11 penicillin V + H2O
-
 uncultured bacterium phenoxyacetate + 6-aminopenicillinate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.11 heterodimer 1 * 26000, alpha-subunit, + 1 * 63000, beta-subunit, recombinant His-tagged enzyme, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 ACPGA001 PGA
-
 uncultured bacterium
3.5.1.11 penicillin G acylase
-
 uncultured bacterium
3.5.1.11 PGA
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 60
-
 recombinant His-tagged enzyme, at pH 8.0 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.11 25 75 activity range uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.11 40
-
 purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 90% activity remaining uncultured bacterium
3.5.1.11 50
-
 purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 50% activity remaining uncultured bacterium
3.5.1.11 55
-
 purified recombinant His-tagged enzyme, pH 7.4, 70 min, over 40% activity remaining, t1/2 is 60 min uncultured bacterium
3.5.1.11 60
-
 purified recombinant His-tagged enzyme, pH 7.4, 15 min, inactivation uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.11 34.2
-
 cephalexin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 48.4
-
 Penicillin V pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 49.5
-
 ampicillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 56.2
-
 amoxycillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 58.3
-
 6-nitro-3-(phenylacetamido)-benzoic acid pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 63.7
-
 carbenicillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 64.2
-
 penicillin G pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 66.9
-
 cephaloridine pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 78.3
-
 cephalothin pH 7.4, 37°C, recombinant enzyme uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 8
-
 recombinant His-tagged enzyme, at 60°C uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.11 4 10 activity range uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.11 6 9 purified recombinant His-tagged enzyme, 30°C, 30 min, over 80% activity within this range uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.11 524.4
-
 ampicillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 534.2
-
 amoxycillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 1227
-
 carbenicillin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 1232
-
 Penicillin V pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 1341
-
 cephalexin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 4079
-
 cephaloridine pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 5019
-
 cephalothin pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 7024
-
 6-nitro-3-(phenylacetamido)-benzoic acid pH 7.4, 37°C, recombinant enzyme uncultured bacterium
3.5.1.11 13960
-
 penicillin G pH 7.4, 37°C, recombinant enzyme uncultured bacterium