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Literature summary extracted from

  • Leungsakul T, L.T.; Keenan B.G, K.B.; Mori M.-A, M.M.; Morton M.D, M.M.; Stuart J.D, S.J.; Smets B.F, S.B.; Wood T.K, W.T.
    Oxidation of aminonitrotoluenes by 2,4-DNT dioxygenase of Burkholderia sp. strain DNT (2006), Biotechnol. Bioeng., 93, 231-237.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.24 expressed in Escherichia coli JVQ2 cells Burkholderia sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.24 I204Y mutation in alpha subunit DntAc, results in two- to fourfold faster oxidization of the aminonitrotoluenes Burkholderia sp.
1.14.12.24 I204Y the mutation results in 2-4fold faster oxidization of the aminonitrotoluenes compared to the wild type enzyme Burkholderia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + O2 Burkholderia sp.
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2,4-dinitrotoluene + NADH + O2 Burkholderia sp. DNT
-
 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.24 Burkholderia sp.
-
-
-
1.14.12.24 Burkholderia sp. Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-
1.14.12.24 Burkholderia sp. DNT
-
-
-
1.14.12.24 Burkholderia sp. DNT Q5BM20 and Q6QUY4 and Q45695 and Q6QUY3 Q5BM20 i.e flavoprotein reductase DntAa, Q6QUY4 i.e iron-sulfur ferredoxin DntAb, Q45695 i.e. large subunit DntAc, Q6QUY3 i.e. small subunit DntAd
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.24 2,4-dinitrotoluene + NADH + O2
-
 Burkholderia sp. 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2,4-dinitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 4-methyl-5-nitrocatechol + nitrite + NAD+
-
 ?
1.14.12.24 2-amino-3-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-amino-3-nitrobenzylalcohol + NAD+ + H2O
-
 ?
1.14.12.24 2-amino-3-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 2-amino-3-nitrobenzylalcohol + NAD+ + H2O
-
 ?
1.14.12.24 2-amino-3-nitrotoluene + NADH + O2
-
 Burkholderia sp. 2-amino-3-nitrobenzylalcohol + nitrite + NAD+ 27% yield ?
1.14.12.24 2-amino-3-nitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 2-amino-3-nitrobenzylalcohol + nitrite + NAD+ 27% yield ?
1.14.12.24 2-amino-5-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
-
 ?
1.14.12.24 2-amino-5-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
-
 ?
1.14.12.24 2-amino-5-nitrotoluene + NADH + O2
-
 Burkholderia sp. 2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+ 34.4% yield ?
1.14.12.24 2-amino-5-nitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+ 34.4% yield ?
1.14.12.24 2-amino-6-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
-
 ?
1.14.12.24 2-amino-6-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. DNT 2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
-
 ?
1.14.12.24 2-amino-6-nitrotoluene + NADH + O2
-
 Burkholderia sp. 2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+ 20% yield ?
1.14.12.24 2-amino-6-nitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+ 20% yield ?
1.14.12.24 3-amino-4-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 3-amino-4-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
-
 ?
1.14.12.24 3-amino-4-nitrotoluene + NADH + O2
-
 Burkholderia sp. 3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+ 9% yield ?
1.14.12.24 3-amino-4-nitrotoluene + NADH + O2
-
 Burkholderia sp. DNT 3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+ 9% yield ?
1.14.12.24 4-amino-2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. aminonitrocresol + NAD+ + H2O
-
 ?
1.14.12.24 4-amino-2-nitrotoluene + NADH + O2 13% yield Burkholderia sp. aminonitrocresol + nitrite + NAD+
-
 ?
1.14.12.24 4-amino-3-nitrotoluene + NADH + O2
-
 Burkholderia sp. 4-amino-3-nitrobenzylalcohol + aminonitrocresol + ?
-
 ?
1.14.12.24 4-amino-3-nitrotoluene + NADH + O2 19% yield Burkholderia sp. 4-amino-3-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
-
 ?
1.14.12.24 5-amino-2-nitrotoluene + NADH + H+ + O2
-
 Burkholderia sp. 2-amino-5-nitro-o-cresol + NAD+ + H2O
-
 ?
1.14.12.24 5-amino-2-nitrotoluene + NADH + O2
-
 Burkholderia sp. 2-amino-5-nitro-o-cresol + nitrite + NAD+ 24% yield ?
1.14.12.24 additional information 2-amino-4-nitrotoluene is not a substrate Burkholderia sp. ?
-
 ?
1.14.12.24 additional information no substrate: 2-amino-4-nitrotoluene Burkholderia sp. ?
-
 ?
1.14.12.24 additional information 2-amino-4-nitrotoluene is not a substrate Burkholderia sp. DNT ?
-
 ?
1.14.12.24 additional information no substrate: 2-amino-4-nitrotoluene Burkholderia sp. DNT ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.24 2,4-DNT dioxygenase
-
 Burkholderia sp.
1.14.12.24 DDO
-
 Burkholderia sp.