Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nandanwar, H.S.; Vohra, R.M.; Hoondal, G.S.
    Trimeric l-N-carbamoylase from newly isolated Brevibacillus reuszeri HSN1: a potential biocatalyst for production of l-?-amino acids (2013), Biotechnol. Appl. Biochem., 60, 219-230.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.87 synthesis the enzyme shows promise as a potential biocatalyst for L-alpha-amino acid production Brevibacillus reuszeri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.87 1,10-phenanthroline 54% inhibition at 2 mM Brevibacillus reuszeri
3.5.1.87 4-hydroxymercuribenzoate complete inhibition at 1 mM Brevibacillus reuszeri
3.5.1.87 EDTA 15% inhibition at 5 mM, 27% at 10 mM Brevibacillus reuszeri
3.5.1.87 Mercuric chloride complete inhibition at 1 mM Brevibacillus reuszeri
3.5.1.87 additional information no inhibition by PMSF and DTT Brevibacillus reuszeri
3.5.1.87 N-bromosuccinimide complete inhibition at 1 mM Brevibacillus reuszeri
3.5.1.87 Zn2+ weak inhibition at 1 mM Brevibacillus reuszeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.87 13.5
-
 N-carbamoyl-L-methionine pH 7.5, 40°C Brevibacillus reuszeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.87 Co2+ activates at 0.08 mM Brevibacillus reuszeri
3.5.1.87 Mn2+ enzyme activity is enhanced by 73% at 0.8 mM Mn2+ Brevibacillus reuszeri
3.5.1.87 additional information no significant effect by Fe2+, Ca2+, and Ni2+ at low concentrations, overview Brevibacillus reuszeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.87 44300
-
 3 * 44300, SDS-PAGE Brevibacillus reuszeri
3.5.1.87 132000
-
 gel filtration Brevibacillus reuszeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.87 an N-carbamoyl-L-2-amino acid + H2O Brevibacillus reuszeri
-
 an L-2-amino acid + NH3 + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.87 Brevibacillus reuszeri
-
-
-
3.5.1.87 Brevibacillus reuszeri HSN1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.87 native enzyme 60fold to homogeneity by heat treatment at 70°C for 2 min, anion exchange chromatography of the supernatant, hydrophobic interaction chromatography, ultrafiltration, and gel filtration Brevibacillus reuszeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.87 145
-
 purified enzyme, pH 7.5, 40°C Brevibacillus reuszeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.87 an N-carbamoyl-L-2-amino acid + H2O
-
 Brevibacillus reuszeri an L-2-amino acid + NH3 + CO2
-
 ?
3.5.1.87 additional information the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate Brevibacillus reuszeri ?
-
 ?
3.5.1.87 additional information the enzyme shows stereospecificity and a broad substrate specificity. No or poor activitz with N-carbamoyl-alpha-alanine, N-carbamoyl-glycine, N-carbamoyl-beta-alanine, and N-carbamoyl-alpha-aminoisobutyrate Brevibacillus reuszeri HSN1 ?
-
 ?
3.5.1.87 N-carbamoyl-D,L-serine + H2O
-
 Brevibacillus reuszeri L-serine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O preferred substrate Brevibacillus reuszeri L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O preferred substrate Brevibacillus reuszeri HSN1 L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-phenylalanine + H2O
-
 Brevibacillus reuszeri L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-phenylalanine + H2O
-
 Brevibacillus reuszeri HSN1 L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-phenylglycine + H2O
-
 Brevibacillus reuszeri L-phenylglycine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tryptophan + H2O
-
 Brevibacillus reuszeri L-tryptophan + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tryptophan + H2O
-
 Brevibacillus reuszeri HSN1 L-tryptophan + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-valine + H2O
-
 Brevibacillus reuszeri L-valine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-valine + H2O
-
 Brevibacillus reuszeri HSN1 L-valine + CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.87 homotrimer 3 * 44300, SDS-PAGE Brevibacillus reuszeri

Synonyms

EC Number Synonyms Comment Organism
3.5.1.87 L-N-carbamoylase
-
 Brevibacillus reuszeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.87 50
-
-
 Brevibacillus reuszeri

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.87 20 75 activity range, profile overview Brevibacillus reuszeri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.87 50
-
 purified enzyme, 60 min, about 86% activity remaining, half-life is 180 min Brevibacillus reuszeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.87 8.5
-
-
 Brevibacillus reuszeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.87 5.5 9 activity range, profile overview Brevibacillus reuszeri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.87 Brevibacillus reuszeri isoelectric focusing
-
 5.45