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Literature summary extracted from

  • Swartz, D.J.; Mok, L.; Botta, S.K.; Singh, A.; Altenberg, G.A.; Urbatsch, I.L.
    Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance (2014), Biosci. Rep., 34, e00116.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.2 expressed in Saccharomyces cerevisiae JPY201 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.2 cyclosporine A
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.6.2.2 120000
-
 x * 120000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.6.2.2 ATP + H2O + xenobiotic/in Homo sapiens
-
 ADP + phosphate + xenobiotic/out
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.2 Ni-NTA resin column chromatography and Superose 6B column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6.2.2 3.5
-
 with verapamil and ATP as substrates, at pH 7.4 and 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.2 ATP + H2O + daunorubicin/in
-
 Homo sapiens ADP + phosphate + daunorubicin/out
-
 ?
7.6.2.2 ATP + H2O + doxorubicin/in
-
 Homo sapiens ADP + phosphate + doxorubicin/out
-
 ?
7.6.2.2 ATP + H2O + verapamil/in
-
 Homo sapiens ADP + phosphate + verapamil/out
-
 ?
7.6.2.2 ATP + H2O + xenobiotic/in
-
 Homo sapiens ADP + phosphate + xenobiotic/out
-
 ?

Subunits

EC Number Subunits Comment Organism
7.6.2.2 ? x * 120000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
7.6.2.2 ABCB1
-
 Homo sapiens
7.6.2.2 MDR1
-
 Homo sapiens
7.6.2.2 P-glycoprotein
-
 Homo sapiens
7.6.2.2 Pgp
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.6.2.2 0.002
-
 cyclosporine A pH and temperature not specified in the publication Homo sapiens