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Literature summary extracted from

  • Samukange, V.; Yasukawa, K.; Inouye, K.
    Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7 (2014), Biosci. Biotechnol. Biochem., 78, 41-48.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.23 heparin porcine intestinal heparin, increases activity by decreasing the Km value, increases the enzyme's thermostability Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.23 recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.23 Cholesterol sulfate non-competitive inhibition, decreases the enzyme's thermostability, inhibition mechanism, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.23 0.019
-
 (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, with 0.05 mM heparin Homo sapiens
3.4.24.23 0.057
-
 (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 pH 7.5, 25°C, without heparin Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.23 Homo sapiens P09237
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.23 proteolytic modification the enzyme is matured by proteolytic cleavage, the mature MMP-7 comprises Tyr78-Lys250 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.23 recombinant solubilized and refolded enzyme from Escherichia coli strain BL21(DE3) inclusion bodies by ammonium sulfate fractionation and heparin affinity chromatography Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.23 recombinant enzyme, solubilization from Escherichia coli strain BL21(DE3) inclusion bodies by 6 M guanidine HCl, and refolded with 1 M L-arginine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.23 (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2 + H2O
-
 Homo sapiens (7-methoxycoumarin-4-yl)-acetyl-L-Pro-L-Leu-Gly + L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl]-L-Ala-L-Arg-NH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.23 Matrix metalloproteinase 7
-
 Homo sapiens
3.4.24.23 MMP-7
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.23 25
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.23 additional information
-
 in thermal incubation of the enzyme at 50-70°C, heparin increases the relative activity (the ratio of kcat/Km of MMP-7 with incubation to that without it), while cholesterol sulfate decreases the relative activity Homo sapiens
3.4.24.23 50 60 purified enzyme with or without heparin and cholesterol sulfate, 10 min, 10-40% activity remaining, overview Homo sapiens
3.4.24.23 70
-
 purified enzyme, inactivation within 10 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.23 7.5
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.23 0.011
-
 Cholesterol sulfate pH 7.5, 25°C Homo sapiens