Any feedback?
Literature summary extracted from
- MALDI-TOF MS and CD spectral analysis for identification and structure prediction of a purified, novel, organic solvent stable, fibrinolytic metalloprotease from Bacillus cereus B80 (2015), BioMed Res. Int., 2015, 527015.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.28 | DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis | Bacillus cereus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.24.28 | additional information | - |
additional information | Michaelis-Menten kinetics | Bacillus cereus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.24.28 | additional information | metalloprotease | Bacillus cereus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 26000 | - |
x * 26000, SDS-PAGE | Bacillus cereus |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.4.24.28 | additional information | the enzyme exhibits strong fibrinolytic, collagenolytic, and gelatinolytic properties and stability in various organic solvents, overview | Bacillus cereus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.28 | Bacillus cereus | - |
- |
- |
| 3.4.24.28 | Bacillus cereus B80 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.28 | native enzyme 13.1fold by ethanol precipitation, gel filtration, and anion exchange chromatography | Bacillus cereus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 3556 | - |
purified enzyme, pH 7.5, 37°C | Bacillus cereus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.28 | Bovine serum albumin + H2O | - |
Bacillus cereus | ? | - |
? |  |
| 3.4.24.28 | Bovine serum albumin + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| 3.4.24.28 | casein + H2O | - |
Bacillus cereus | ? | - |
? | |
| 3.4.24.28 | casein + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| 3.4.24.28 | Collagen + H2O | - |
Bacillus cereus | ? | - |
? | |
| 3.4.24.28 | Collagen + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| 3.4.24.28 | fibrinogen + H2O | - |
Bacillus cereus | fibrin + ? | - |
? | |
| 3.4.24.28 | Gelatin + H2O | - |
Bacillus cereus | ? | - |
? | |
| 3.4.24.28 | Gelatin + H2O | - |
Bacillus cereus B80 | ? | - |
? | |
| 3.4.24.28 | additional information | the enzyme is able to hydrolyze various proteins with highest affinity towards casein followed by bovine serum albumin and gelatin | Bacillus cereus | ? | - |
? | |
| 3.4.24.28 | additional information | the enzyme is able to hydrolyze various proteins with highest affinity towards casein followed by bovine serum albumin and gelatin | Bacillus cereus B80 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.24.28 | ? | x * 26000, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 60 | - |
- |
Bacillus cereus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 30 | 70 | activity range | Bacillus cereus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 30 | - |
purified enzyme, 2 h, stable | Bacillus cereus |
| 3.4.24.28 | 40 | 50 | purified enzyme, 2 h, loss of 16-22% activity | Bacillus cereus |
| 3.4.24.28 | 60 | - |
purified enzyme, loss of 30% activity after 1 h, and loss of 56% activity after 2 h | Bacillus cereus |
| 3.4.24.28 | 70 | - |
purified enzyme, loss of 50% activity after 1 h | Bacillus cereus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 8 | - |
- |
Bacillus cereus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 6 | 10 | activity range | Bacillus cereus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.28 | 6 | 9 | purified enzyme, 60°C, loss of 20-25% activity after 10 min | Bacillus cereus |
| 3.4.24.28 | 10 | - |
purified enzyme, 60°C, loss of 40% activity after 10 min | Bacillus cereus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.24.28 | evolution | the enzyme exhibits 70-93% similarity with zinc metalloproteases from various strains Bacillus sp. specifically from Bacillus cereus group, 22-24% identity with bacillolysin from Bacillus cereus, mass spectrometry | Bacillus cereus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
