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Literature summary extracted from

  • Osman, W.; Endo, S.; Oh-Hashi, K.; Kitamura, Y.; Kitade, Y.
    Molecular characterization and mutational analysis of recombinant diadenosine 5',5''-P1,P4-tetraphosphate hydrolase from Plasmodium falciparum (2012), Biol. Pharm. Bull., 35, 1191-1196.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.17 expression in Escherichia coli Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.17 P133A 5fold increase in Km value Plasmodium falciparum
3.6.1.17 P133F 3fold decrease in kcat value Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.17 0.0006
-
 5',5''-diadenosine tetraphosphate wild-type, pH 7.5, 22°C Plasmodium falciparum
3.6.1.17 0.0008
-
 5',5''-diadenosine tetraphosphate mutant P133F, pH 7.5, 22°C Plasmodium falciparum
3.6.1.17 0.003
-
 5',5''-diadenosine tetraphosphate mutant P133A, pH 7.5, 22°C Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.17 Co2+ 5 mM, 42% of the activity with Mg2+ Plasmodium falciparum
3.6.1.17 Mg2+ best activator, maximum activity at 5 mM Plasmodium falciparum
3.6.1.17 Mn2+ 5 mM, 11% of the activity with Mg2+ Plasmodium falciparum
3.6.1.17 additional information no activation by Ca2+ Plasmodium falciparum
3.6.1.17 Zn2+ 5 mM, 2% of the activity with Mg2+ Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.17 17000
-
 x * 17000, SDS-PAGE Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.17 Plasmodium falciparum C0H4F3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.17 5',5''-diadenosine pentaphosphate + H2O
-
 Plasmodium falciparum ATP + ADP 42% of the activity with 5',5''-diadenosine tetraphosphate ?
3.6.1.17 5',5''-diadenosine tetraphosphate + H2O
-
 Plasmodium falciparum ATP + AMP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.17 ? x * 17000, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
3.6.1.17 PFE1035c
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.17 60
-
-
 Plasmodium falciparum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.17 70
-
 10 min, 39% of initial activity Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.17 0.013
-
 5',5''-diadenosine tetraphosphate mutant P133F, pH 7.5, 22°C Plasmodium falciparum
3.6.1.17 0.042
-
 5',5''-diadenosine tetraphosphate wild-type, pH 7.5, 22°C Plasmodium falciparum
3.6.1.17 0.08
-
 5',5''-diadenosine tetraphosphate mutant P133A, pH 7.5, 22°C Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.17 7.5
-
-
 Plasmodium falciparum