BRENDA - Enzyme Database

Molecular characterization and mutational analysis of recombinant diadenosine 5',5''-P1,P4-tetraphosphate hydrolase from Plasmodium falciparum

Osman, W.; Endo, S.; Oh-Hashi, K.; Kitamura, Y.; Kitade, Y.; Biol. Pharm. Bull. 35, 1191-1196 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.17
expression in Escherichia coli
Plasmodium falciparum
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.17
P133A
5fold increase in Km value
Plasmodium falciparum
3.6.1.17
P133F
3fold decrease in kcat value
Plasmodium falciparum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0006
-
5',5''-diadenosine tetraphosphate
wild-type, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.0008
-
5',5''-diadenosine tetraphosphate
mutant P133F, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.003
-
5',5''-diadenosine tetraphosphate
mutant P133A, pH 7.5, 22°C
Plasmodium falciparum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Co2+
5 mM, 42% of the activity with Mg2+
Plasmodium falciparum
3.6.1.17
Mg2+
best activator, maximum activity at 5 mM
Plasmodium falciparum
3.6.1.17
Mn2+
5 mM, 11% of the activity with Mg2+
Plasmodium falciparum
3.6.1.17
additional information
no activation by Ca2+
Plasmodium falciparum
3.6.1.17
Zn2+
5 mM, 2% of the activity with Mg2+
Plasmodium falciparum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
17000
-
x * 17000, SDS-PAGE
Plasmodium falciparum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.17
Plasmodium falciparum
C0H4F3
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
5',5''-diadenosine pentaphosphate + H2O
-
733450
Plasmodium falciparum
ATP + ADP
42% of the activity with 5',5''-diadenosine tetraphosphate
-
-
?
3.6.1.17
5',5''-diadenosine tetraphosphate + H2O
-
733450
Plasmodium falciparum
ATP + AMP
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.6.1.17
?
x * 17000, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.17
60
-
-
Plasmodium falciparum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.6.1.17
70
-
10 min, 39% of initial activity
Plasmodium falciparum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.013
-
5',5''-diadenosine tetraphosphate
mutant P133F, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.042
-
5',5''-diadenosine tetraphosphate
wild-type, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.08
-
5',5''-diadenosine tetraphosphate
mutant P133A, pH 7.5, 22°C
Plasmodium falciparum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7.5
-
-
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.17
expression in Escherichia coli
Plasmodium falciparum
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.6.1.17
P133A
5fold increase in Km value
Plasmodium falciparum
3.6.1.17
P133F
3fold decrease in kcat value
Plasmodium falciparum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.0006
-
5',5''-diadenosine tetraphosphate
wild-type, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.0008
-
5',5''-diadenosine tetraphosphate
mutant P133F, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.003
-
5',5''-diadenosine tetraphosphate
mutant P133A, pH 7.5, 22°C
Plasmodium falciparum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.6.1.17
Co2+
5 mM, 42% of the activity with Mg2+
Plasmodium falciparum
3.6.1.17
Mg2+
best activator, maximum activity at 5 mM
Plasmodium falciparum
3.6.1.17
Mn2+
5 mM, 11% of the activity with Mg2+
Plasmodium falciparum
3.6.1.17
additional information
no activation by Ca2+
Plasmodium falciparum
3.6.1.17
Zn2+
5 mM, 2% of the activity with Mg2+
Plasmodium falciparum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.6.1.17
17000
-
x * 17000, SDS-PAGE
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.17
5',5''-diadenosine pentaphosphate + H2O
-
733450
Plasmodium falciparum
ATP + ADP
42% of the activity with 5',5''-diadenosine tetraphosphate
-
-
?
3.6.1.17
5',5''-diadenosine tetraphosphate + H2O
-
733450
Plasmodium falciparum
ATP + AMP
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.6.1.17
?
x * 17000, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.6.1.17
60
-
-
Plasmodium falciparum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.6.1.17
70
-
10 min, 39% of initial activity
Plasmodium falciparum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.6.1.17
0.013
-
5',5''-diadenosine tetraphosphate
mutant P133F, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.042
-
5',5''-diadenosine tetraphosphate
wild-type, pH 7.5, 22°C
Plasmodium falciparum
3.6.1.17
0.08
-
5',5''-diadenosine tetraphosphate
mutant P133A, pH 7.5, 22°C
Plasmodium falciparum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.17
7.5
-
-
Plasmodium falciparum