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Literature summary extracted from

  • Peng, W.F.; Huang, C.Y.
    Allantoinase and dihydroorotase binding and inhibition by flavonols and the substrates of cyclic amidohydrolases (2014), Biochimie, 101, 113-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.3 recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae
3.5.2.5 recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.3 galangin
-
 Klebsiella pneumoniae
3.5.2.3 kaempferol
-
 Klebsiella pneumoniae
3.5.2.3 additional information binding and inhibition of allantoinase dihydroorotase by flavonols and the substrates of other cyclic amidohydrolases, dissociation constants, docking analysis using three-dimensional structure model, PDB ID 3JZE, overview. Hydantoin and allantoin bind to dihydroorotase, but do not affect its activity, no inhibition by phthalimide Klebsiella pneumoniae
3.5.2.3 myricetin
-
 Klebsiella pneumoniae
3.5.2.3 quercetin
-
 Klebsiella pneumoniae
3.5.2.5 dihydroorotate competitive inhibition Salmonella enterica
3.5.2.5 galangin
-
 Salmonella enterica
3.5.2.5 hydantoin competitive inhibition Salmonella enterica
3.5.2.5 kaempferol
-
 Salmonella enterica
3.5.2.5 additional information binding and inhibition of allantoinase by flavonols and the substrates of other cyclic amidohydrolases, dissociation constants, docking analysis using three-dimensional structure model, PDB ID 3HM7, overview Salmonella enterica
3.5.2.5 myricetin
-
 Salmonella enterica
3.5.2.5 quercetin
-
 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.3 additional information
-
 additional information Michaelis-Menten kinetics Klebsiella pneumoniae
3.5.2.5 additional information
-
 additional information Michaelis-Menten kinetics Salmonella enterica
3.5.2.5 85.9
-
 (S)-allantoin pH 8.0, 25°C, recombinant enzyme Salmonella enterica
3.5.2.5 86
-
 (S)-allantoin pH 8.0, 25°C, recombinant enzyme, in presence of phthalimide Salmonella enterica
3.5.2.5 108.3
-
 (S)-allantoin pH 8.0, 25°C, recombinant enzyme, in presence of hydantoin Salmonella enterica
3.5.2.5 129.6
-
 (S)-allantoin pH 8.0, 25°C, recombinant enzyme, in presence of dihydroorotate Salmonella enterica
3.5.2.5 231.6
-
 (S)-allantoin pH 8.0, 25°C, recombinant enzyme, in presence of kaempferol Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.3 additional information binuclear metal center within the active site Klebsiella pneumoniae
3.5.2.5 additional information binuclear metal center within the active site Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.3 Klebsiella pneumoniae
-
-
-
3.5.2.5 Salmonella enterica
-
 serovar typhimurium LT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.3 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Klebsiella pneumoniae
3.5.2.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Salmonella enterica

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.2.3 (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate catalytic mechanism, overview Klebsiella pneumoniae
a
3.5.2.5 (S)-allantoin + H2O = allantoate catalytic mechanism, overview Salmonella enterica
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.3 (S)-dihydroorotate + H2O absolute substrate specificity Klebsiella pneumoniae N-carbamoyl-L-aspartate
-
 ?
3.5.2.3 additional information allantoin, hydantoin, and phthalimide are not hydrolyzed by dihydroorotase Klebsiella pneumoniae ?
-
 ?
3.5.2.5 (S)-allantoin + H2O absolute substrate specificity Salmonella enterica allantoate
-
 ?
3.5.2.5 additional information dihydroorotate, hydantoin, and phthalimide are not hydrolyzed by allantoinase Salmonella enterica ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.3 25
-
 assay at Klebsiella pneumoniae
3.5.2.5 25
-
 assay at Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.3 8
-
 assay at Klebsiella pneumoniae
3.5.2.5 8
-
 assay at Salmonella enterica

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.2.3 0.031
-
 pH 8.0, 25°C, recombinant enzyme Klebsiella pneumoniae kaempferol
3.5.2.3 0.04
-
 pH 8.0, 25°C, recombinant enzyme Klebsiella pneumoniae myricetin
3.5.2.5 0.035
-
 pH 8.0, 25°C, recombinant enzyme Salmonella enterica kaempferol
3.5.2.5 18.6
-
 pH 8.0, 25°C, recombinant enzyme Salmonella enterica dihydroorotate
3.5.2.5 230
-
 pH 8.0, 25°C, recombinant enzyme Salmonella enterica hydantoin

General Information

EC Number General Information Comment Organism
3.5.2.3 additional information the enzyme contains four histidine, one aspartate, and one post-carboxylated lysine residue, which are required for metal binding and catalytic activity Klebsiella pneumoniae