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Literature summary extracted from

  • Huxley, L.; Quirk, P.G.; Cotton, N.P.J.; White, S.A.; Jackson, J.B.
    The specificity of proton-translocating transhydrogenase for nicotinamide nucleotides (2011), Biochim. Biophys. Acta, 1807, 85-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.1 expressed in Escherichia coli C600 and BL21(DE3) cells Rhodospirillum rubrum

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.1 E155W the mutant shows wild type activity Rhodospirillum rubrum
7.1.1.1 E155W/R165A the mutations mutation do not significantly affect catalytic activity Rhodospirillum rubrum
7.1.1.1 R165A a higher concentration of the nucleotide is needed to achieve the half-maximal rate compared to with the wild type protein Rhodospirillum rubrum
7.1.1.1 W72F the mutant shows wild type activity Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.1 membrane
-
 Rhodospirillum rubrum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+/in Rhodospirillum rubrum
-
 NADP+ + NADH + H+/out
-
 r

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.1 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.1
-
 Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.1 NADPH + NAD+ + H+/in
-
 Rhodospirillum rubrum NADP+ + NADH + H+/out
-
 r
7.1.1.1 NADPH + oxidized acetyl pyridine adenine dinucleotide + H+/in
-
 Rhodospirillum rubrum NADP+ + reduced acetyl pyridine adenine dinucleotide + H+/out
-
 r

Synonyms

EC Number Synonyms Comment Organism
7.1.1.1 proton-translocating transhydrogenase
-
 Rhodospirillum rubrum