Literature summary extracted from
Zhang, J.; Hwang, T.C.
The fifth transmembrane segment of cystic fibrosis transmembrane conductance regulator contributes to its anion permeation pathway (2015), Biochemistry, 54, 3839-3850.
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.6.1.6 |
A299C |
site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate |
Homo sapiens |
5.6.1.6 |
A326C |
site-directed mutagenesis, the substituted cysteine does not respond to neither internal nor external 2-sulfonatoethyl methanethiosulfonate and is inaccessible to channel-permeant thiol-specific reagent [Au(CN)2]- |
Homo sapiens |
5.6.1.6 |
F310C |
site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate |
Homo sapiens |
5.6.1.6 |
F311C |
site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate |
Homo sapiens |
5.6.1.6 |
L323C |
site-directed mutagenesis, the substituted cysteine does not respond to neither internal nor external 2-sulfonatoethyl methanethiosulfonate and is inaccessible to channel-permeant thiol-specific reagent [Au(CN)2]- |
Homo sapiens |
5.6.1.6 |
N306C |
site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate |
Homo sapiens |
5.6.1.6 |
R303C |
site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate |
Homo sapiens |
5.6.1.6 |
S307C |
site-directed mutagenesis, the engineered cysteine reacts with intracellular 2-sulfonatoethyl methanethiosulfonate |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.6.1.6 |
2-sulfonatoethyl methanethiosulfonate |
enzyme residues A299, R303, N306, S307, F310, and F311 are accessible to intracellular 2-sulfonatoethyl methanethiosulfonate in both the open and closed states |
Homo sapiens |
|
5.6.1.6 |
glibencalmide |
an open-channel blocker |
Homo sapiens |
|
5.6.1.6 |
[Au(CN)2]- |
a channel-permeant thiol-specific reagent |
Homo sapiens |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
5.6.1.6 |
membrane |
the enzyme possesses two transmembrane domains, each of which comprises six transmembrane segments. Function of several transmembrane segments (TMs), including TM1, TM3, TM6, TM9, TM11, and TM12, as pore-lining segments the enzyme. Screening of the entire transmembrane segment 5 defined by the original topology model and its cytoplasmic extension in a Cys-less background, the segment of TM5 between F311 and L323 is concealed from the pore by other TMs and/or lipid bilayers, the cytoplasmic portion of TM5 lines the pore |
Homo sapiens |
16020 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.6.1.6 |
Homo sapiens |
P13569 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.6.1.6 |
CFTR |
- |
Homo sapiens |
5.6.1.6 |
cystic fibrosis transmembrane conductance regulator |
- |
Homo sapiens |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
5.6.1.6 |
additional information |
enzyme residues A299, R303, N306, S307, F310, and F311 are accessible to intracellular 2-sulfonatoethyl methanethiosulfonate in both the open and closed states. The segment of transmembrane segment 5 between F311 and L323 is concealed from the pore by other TMs and/or lipid bilayers, the cytoplasmic portion of TM5 lines the pore |
Homo sapiens |