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Literature summary extracted from

  • Gannavaram, S.; Sirin, S.; Sherman, W.; Gadda, G.
    Mechanistic and computational studies of the reductive half-reaction of tyrosine to phenylalanine active site variants of D-arginine dehydrogenase (2014), Biochemistry, 53, 6574-6583.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.99.6
-
 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.4.99.6 Y249F steady-state kinetic parameters similar to those of the wild-type enzyme. Rate constants for flavin reduction (kred) with D-leucine are 3fold smaller than the wild-type value with similar pKa values for an unprotonated group of about 10.0. Cleavage of the substrate NH and CH bonds in the enzyme variant occurs in synchronous fashion, which can be reconciled with a hydride transfer mechanism Pseudomonas aeruginosa
1.4.99.6 Y53F steady-state kinetic parameters similar to those of the wild-type enzyme. Rate constants for flavin reduction (kred) with D-leucineare 3fold smaller than the wild-type value with similar pKa values for an unprotonated group of about 10.0. Cleavage of the substrate NH and CH bonds in the enzyme variant occurs in synchronous fashion, which can be reconciled with a hydride transfer mechanism Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.99.6 0.06
-
 D-arginine mutant Y249F, pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 0.36
-
 D-arginine mutant Y53F, pH 8.7, 25°C Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.6 Pseudomonas aeruginosa Q9HXE3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.6 D-arginine + phenazine methosulfate
-
 Pseudomonas aeruginosa 5-guanidino-2-oxopentanoate + NH3 + reduced phenazine methosulfate overall reaction ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.99.6 204
-
 D-arginine mutant Y249F, pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 420
-
 D-arginine mutant Y53F, pH 8.7, 25°C Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.99.6 1200
-
 D-arginine mutant Y53F, pH 8.7, 25°C Pseudomonas aeruginosa
1.4.99.6 3400
-
 D-arginine mutant Y249F, pH 8.7, 25°C Pseudomonas aeruginosa