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Literature summary extracted from

  • Dreyton, C.J.; Knuckley, B.; Jones, J.E.; Lewallen, D.M.; Thompson, P.R.
    Mechanistic studies of protein arginine deiminase 2: evidence for a substrate-assisted mechanism (2014), Biochemistry, 53, 4426-4433.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.15 expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.15 0.24
-
 Nalpha-benzoyl-L-arginine methyl ester at pH 7.6 and 37°C Homo sapiens
3.5.3.15 0.27
-
 Nalpha-benzoyl-L-arginine ethyl ester at pH 7.6 and 37°C Homo sapiens
3.5.3.15 0.48
-
 Nalpha-benzoyl-L-arginine amide at pH 7.6 and 37°C Homo sapiens
3.5.3.15 1.6
-
 Nalpha-benzoyl-L-arginine at pH 7.6 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.15 Ca2+ dependent on, the concentration of calcium that is required for half-maximal activity is 0.2 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.15 protein L-arginine + H2O Homo sapiens
-
 protein L-citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Homo sapiens Q9Y2J8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.15 Ni-NTA-agarose resin, column chromatography, and gel filtration Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.5.3.15 -80°C, in 20 mM Tris-HCl, pH 7.6, 1 mM EDTA, 500 mM NaCl, 0.5M tris(2-carboxyethyl) phosphine, and 10% (v/v) glycerol, several months, the enzyme remains stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 additional information nearly no activity with free L-arginine, L-agmatine, histone H3, and histone H4 Homo sapiens ?
-
 ?
3.5.3.15 Nalpha-benzoyl-L-arginine + H2O
-
 Homo sapiens Nalpha-benzoyl-L-citrulline + NH3
-
 ?
3.5.3.15 Nalpha-benzoyl-L-arginine amide + H2O
-
 Homo sapiens Nalpha-benzoyl-L-citrulline amide + NH3
-
 ?
3.5.3.15 Nalpha-benzoyl-L-arginine ethyl ester + H2O 100% activity Homo sapiens Nalpha-benzoyl-L-citrulline ethyl ester + NH3
-
 ?
3.5.3.15 Nalpha-benzoyl-L-arginine methyl ester + H2O
-
 Homo sapiens Nalpha-benzoyl-L-citrulline methyl ester + NH3
-
 ?
3.5.3.15 protein L-arginine + H2O
-
 Homo sapiens protein L-citrulline + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.15 PAD2
-
 Homo sapiens
3.5.3.15 protein arginine deiminase 2
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.15 0.32
-
 Nalpha-benzoyl-L-arginine amide at pH 7.6 and 37°C Homo sapiens
3.5.3.15 0.43
-
 Nalpha-benzoyl-L-arginine methyl ester at pH 7.6 and 37°C Homo sapiens
3.5.3.15 0.63
-
 Nalpha-benzoyl-L-arginine at pH 7.6 and 37°C Homo sapiens
3.5.3.15 3.2
-
 Nalpha-benzoyl-L-arginine ethyl ester at pH 7.6 and 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.15 7.6
-
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.15 0.39
-
 Nalpha-benzoyl-L-arginine at pH 7.6 and 37°C Homo sapiens
3.5.3.15 0.68
-
 Nalpha-benzoyl-L-arginine amide at pH 7.6 and 37°C Homo sapiens
3.5.3.15 1.8
-
 Nalpha-benzoyl-L-arginine methyl ester at pH 7.6 and 37°C Homo sapiens
3.5.3.15 11.7
-
 Nalpha-benzoyl-L-arginine ethyl ester at pH 7.6 and 37°C Homo sapiens